Differential expression of gill Na + ,K + -ATPase alpha- and beta-subunits, Na + ,K + ,2Cl - cotransporter and CFTR anion channel in juvenile anadromous and landlocked Atlantic salmon Salmo salar
Udgivelsesdato: August This study examines changes in gill Na(+),K(+)-ATPase (NKA) alpha- and beta-subunit isoforms, Na(+),K(+),2Cl(-) cotransporter (NKCC) and cystic fibrosis transmembrane conductance regulator (CFTR I and II) in anadromous and landlocked strains of Atlantic salmon during parr-smol...
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2007
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Online Access: | https://portal.findresearcher.sdu.dk/da/publications/09f71550-d96e-11dc-860c-000ea68e967b https://doi.org/10.1242/jeb.002873 |
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ftsydanskunivpub:oai:sdu.dk:publications/09f71550-d96e-11dc-860c-000ea68e967b 2024-09-15T17:56:23+00:00 Differential expression of gill Na + ,K + -ATPase alpha- and beta-subunits, Na + ,K + ,2Cl - cotransporter and CFTR anion channel in juvenile anadromous and landlocked Atlantic salmon Salmo salar Nilsen, Tom O. Ebbesson, Lars O. E. Madsen, Steffen S. McCormick, Stephen D. Andersson, Eva Björnsson, Björn Th. Prunet, Patrick Stefansson, Sigurd O. 2007-08-01 https://portal.findresearcher.sdu.dk/da/publications/09f71550-d96e-11dc-860c-000ea68e967b https://doi.org/10.1242/jeb.002873 eng eng https://portal.findresearcher.sdu.dk/da/publications/09f71550-d96e-11dc-860c-000ea68e967b info:eu-repo/semantics/restrictedAccess Nilsen , T O , Ebbesson , L O E , Madsen , S S , McCormick , S D , Andersson , E , Björnsson , B T , Prunet , P & Stefansson , S O 2007 , ' Differential expression of gill Na + ,K + -ATPase alpha- and beta-subunits, Na + ,K + ,2Cl - cotransporter and CFTR anion channel in juvenile anadromous and landlocked Atlantic salmon Salmo salar ' , Journal of Experimental Biology , vol. 210 , no. Pt 16 , pp. 2885-96 . https://doi.org/10.1242/jeb.002873 Cystic Fibrosis Transmembrane Conductance Regulator Gene Expression Regulation Gills Protein Isoforms Protein Subunits RNA Messenger Salmo salar Sodium-Potassium-Chloride Symporters Sodium-Potassium-Exchanging ATPase Water-Electrolyte Balance article 2007 ftsydanskunivpub https://doi.org/10.1242/jeb.002873 2024-07-22T23:48:45Z Udgivelsesdato: August This study examines changes in gill Na(+),K(+)-ATPase (NKA) alpha- and beta-subunit isoforms, Na(+),K(+),2Cl(-) cotransporter (NKCC) and cystic fibrosis transmembrane conductance regulator (CFTR I and II) in anadromous and landlocked strains of Atlantic salmon during parr-smolt transformation, and after seawater (SW) transfer in May/June. Gill NKA activity increased from February through April, May and June among both strains in freshwater (FW), with peak enzyme activity in the landlocked salmon being 50% below that of the anadromous fish in May and June. Gill NKA-alpha1b, -alpha3, -beta(1) and NKCC mRNA levels in anadromous salmon increased transiently, reaching peak levels in smolts in April/May, whereas no similar smolt-related upregulation of these transcripts occurred in juvenile landlocked salmon. Gill NKA-alpha1a mRNA decreased significantly in anadromous salmon from February through June, whereas alpha1a levels in landlocked salmon, after an initial decrease in April, remained significantly higher than those of the anadromous smolts in May and June. Following SW transfer, gill NKA-alpha1b and NKCC mRNA increased in both strains, whereas NKA-alpha1a decreased. Both strains exhibited a transient increase in gill NKA alpha-protein abundance, with peak levels in May. Gill alpha-protein abundance was lower in SW than corresponding FW values in June. Gill NKCC protein abundance increased transiently in anadromous fish, with peak levels in May, whereas a slight increase was observed in landlocked salmon in May, increasing to peak levels in June. Gill CFTR I mRNA levels increased significantly from February to April in both strains, followed by a slight, though not significant increase in May and June. CFTR I mRNA levels were significantly lower in landlocked than anadromous salmon in April/June. Gill CFTR II mRNA levels did not change significantly in either strain. Our findings demonstrates that differential expression of gill NKA-alpha1a, -alpha1b and -alpha3 isoforms may be important ... Article in Journal/Newspaper Atlantic salmon Salmo salar University of Southern Denmark Research Portal Journal of Experimental Biology 210 16 2885 2896 |
institution |
Open Polar |
collection |
University of Southern Denmark Research Portal |
op_collection_id |
ftsydanskunivpub |
language |
English |
topic |
Cystic Fibrosis Transmembrane Conductance Regulator Gene Expression Regulation Gills Protein Isoforms Protein Subunits RNA Messenger Salmo salar Sodium-Potassium-Chloride Symporters Sodium-Potassium-Exchanging ATPase Water-Electrolyte Balance |
spellingShingle |
Cystic Fibrosis Transmembrane Conductance Regulator Gene Expression Regulation Gills Protein Isoforms Protein Subunits RNA Messenger Salmo salar Sodium-Potassium-Chloride Symporters Sodium-Potassium-Exchanging ATPase Water-Electrolyte Balance Nilsen, Tom O. Ebbesson, Lars O. E. Madsen, Steffen S. McCormick, Stephen D. Andersson, Eva Björnsson, Björn Th. Prunet, Patrick Stefansson, Sigurd O. Differential expression of gill Na + ,K + -ATPase alpha- and beta-subunits, Na + ,K + ,2Cl - cotransporter and CFTR anion channel in juvenile anadromous and landlocked Atlantic salmon Salmo salar |
topic_facet |
Cystic Fibrosis Transmembrane Conductance Regulator Gene Expression Regulation Gills Protein Isoforms Protein Subunits RNA Messenger Salmo salar Sodium-Potassium-Chloride Symporters Sodium-Potassium-Exchanging ATPase Water-Electrolyte Balance |
description |
Udgivelsesdato: August This study examines changes in gill Na(+),K(+)-ATPase (NKA) alpha- and beta-subunit isoforms, Na(+),K(+),2Cl(-) cotransporter (NKCC) and cystic fibrosis transmembrane conductance regulator (CFTR I and II) in anadromous and landlocked strains of Atlantic salmon during parr-smolt transformation, and after seawater (SW) transfer in May/June. Gill NKA activity increased from February through April, May and June among both strains in freshwater (FW), with peak enzyme activity in the landlocked salmon being 50% below that of the anadromous fish in May and June. Gill NKA-alpha1b, -alpha3, -beta(1) and NKCC mRNA levels in anadromous salmon increased transiently, reaching peak levels in smolts in April/May, whereas no similar smolt-related upregulation of these transcripts occurred in juvenile landlocked salmon. Gill NKA-alpha1a mRNA decreased significantly in anadromous salmon from February through June, whereas alpha1a levels in landlocked salmon, after an initial decrease in April, remained significantly higher than those of the anadromous smolts in May and June. Following SW transfer, gill NKA-alpha1b and NKCC mRNA increased in both strains, whereas NKA-alpha1a decreased. Both strains exhibited a transient increase in gill NKA alpha-protein abundance, with peak levels in May. Gill alpha-protein abundance was lower in SW than corresponding FW values in June. Gill NKCC protein abundance increased transiently in anadromous fish, with peak levels in May, whereas a slight increase was observed in landlocked salmon in May, increasing to peak levels in June. Gill CFTR I mRNA levels increased significantly from February to April in both strains, followed by a slight, though not significant increase in May and June. CFTR I mRNA levels were significantly lower in landlocked than anadromous salmon in April/June. Gill CFTR II mRNA levels did not change significantly in either strain. Our findings demonstrates that differential expression of gill NKA-alpha1a, -alpha1b and -alpha3 isoforms may be important ... |
format |
Article in Journal/Newspaper |
author |
Nilsen, Tom O. Ebbesson, Lars O. E. Madsen, Steffen S. McCormick, Stephen D. Andersson, Eva Björnsson, Björn Th. Prunet, Patrick Stefansson, Sigurd O. |
author_facet |
Nilsen, Tom O. Ebbesson, Lars O. E. Madsen, Steffen S. McCormick, Stephen D. Andersson, Eva Björnsson, Björn Th. Prunet, Patrick Stefansson, Sigurd O. |
author_sort |
Nilsen, Tom O. |
title |
Differential expression of gill Na + ,K + -ATPase alpha- and beta-subunits, Na + ,K + ,2Cl - cotransporter and CFTR anion channel in juvenile anadromous and landlocked Atlantic salmon Salmo salar |
title_short |
Differential expression of gill Na + ,K + -ATPase alpha- and beta-subunits, Na + ,K + ,2Cl - cotransporter and CFTR anion channel in juvenile anadromous and landlocked Atlantic salmon Salmo salar |
title_full |
Differential expression of gill Na + ,K + -ATPase alpha- and beta-subunits, Na + ,K + ,2Cl - cotransporter and CFTR anion channel in juvenile anadromous and landlocked Atlantic salmon Salmo salar |
title_fullStr |
Differential expression of gill Na + ,K + -ATPase alpha- and beta-subunits, Na + ,K + ,2Cl - cotransporter and CFTR anion channel in juvenile anadromous and landlocked Atlantic salmon Salmo salar |
title_full_unstemmed |
Differential expression of gill Na + ,K + -ATPase alpha- and beta-subunits, Na + ,K + ,2Cl - cotransporter and CFTR anion channel in juvenile anadromous and landlocked Atlantic salmon Salmo salar |
title_sort |
differential expression of gill na + ,k + -atpase alpha- and beta-subunits, na + ,k + ,2cl - cotransporter and cftr anion channel in juvenile anadromous and landlocked atlantic salmon salmo salar |
publishDate |
2007 |
url |
https://portal.findresearcher.sdu.dk/da/publications/09f71550-d96e-11dc-860c-000ea68e967b https://doi.org/10.1242/jeb.002873 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
Nilsen , T O , Ebbesson , L O E , Madsen , S S , McCormick , S D , Andersson , E , Björnsson , B T , Prunet , P & Stefansson , S O 2007 , ' Differential expression of gill Na + ,K + -ATPase alpha- and beta-subunits, Na + ,K + ,2Cl - cotransporter and CFTR anion channel in juvenile anadromous and landlocked Atlantic salmon Salmo salar ' , Journal of Experimental Biology , vol. 210 , no. Pt 16 , pp. 2885-96 . https://doi.org/10.1242/jeb.002873 |
op_relation |
https://portal.findresearcher.sdu.dk/da/publications/09f71550-d96e-11dc-860c-000ea68e967b |
op_rights |
info:eu-repo/semantics/restrictedAccess |
op_doi |
https://doi.org/10.1242/jeb.002873 |
container_title |
Journal of Experimental Biology |
container_volume |
210 |
container_issue |
16 |
container_start_page |
2885 |
op_container_end_page |
2896 |
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1810432586722312192 |