Lipases/Acyltransferases for Lipid Modification in Aqueous Media
Lipases/acyltransferases are a special group of lipases with highly enhanced affinity for other nucleophiles than water compared to classical lipases. In the presence of a suitable acceptor, they catalyze acyltransfer reactions (such as alcoholysis, aminolysis, or perhydrolysis) much faster than hyd...
Main Authors: | , , |
---|---|
Other Authors: | , |
Format: | Book Part |
Language: | English |
Published: |
HAL CCSD
2018
|
Subjects: | |
Online Access: | https://hal.science/hal-01918043 https://doi.org/10.1016/B978-0-12-813167-1.00003-7 |
id |
ftsupagro:oai:HAL:hal-01918043v1 |
---|---|
record_format |
openpolar |
spelling |
ftsupagro:oai:HAL:hal-01918043v1 2023-07-30T03:58:00+02:00 Lipases/Acyltransferases for Lipid Modification in Aqueous Media Subileau, Maeva Jan, Anne Hélène Dubreucq, Eric Ingénierie des Agro-polymères et Technologies Émergentes (UMR IATE) Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA)-Université Montpellier 2 - Sciences et Techniques (UM2)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Université de Montpellier (UM)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro) 2018 https://hal.science/hal-01918043 https://doi.org/10.1016/B978-0-12-813167-1.00003-7 en eng HAL CCSD Academic Press info:eu-repo/semantics/altIdentifier/doi/10.1016/B978-0-12-813167-1.00003-7 ISBN: 9780128131671 hal-01918043 https://hal.science/hal-01918043 doi:10.1016/B978-0-12-813167-1.00003-7 PRODINRA: 451069 Lipid Modification by Enzymes and Engineered Microbes https://hal.science/hal-01918043 Lipid Modification by Enzymes and Engineered Microbes, Academic Press, 2018, 9780128131671. ⟨10.1016/B978-0-12-813167-1.00003-7⟩ Acyltransfer Alcoholysis Biocatalysis CAL-A from Moeziomyces antarcticus (Candida antarctica) CpLIP2 from Candida parapsilosis Structure/function Transesterification aqueous medium acétyltransférase hydrolase milieu aqueux lipase [SDV.IDA]Life Sciences [q-bio]/Food engineering info:eu-repo/semantics/bookPart Book sections 2018 ftsupagro https://doi.org/10.1016/B978-0-12-813167-1.00003-7 2023-07-08T09:08:40Z Lipases/acyltransferases are a special group of lipases with highly enhanced affinity for other nucleophiles than water compared to classical lipases. In the presence of a suitable acceptor, they catalyze acyltransfer reactions (such as alcoholysis, aminolysis, or perhydrolysis) much faster than hydrolysis even in aqueous media with a high thermodynamic activity of water (aW). This allows kinetically controlled reactions where the donor ester is fully converted into the ester product before any significant release of free fatty acids. This chapter will discuss the kinetic aspects of the competitive reactions catalyzed by lipases/acyltransferases, propose a model, and a procedure to quantify their acyltransferase character, present some applications, and give an overview of currently known lipases/acyltransferases and of the structural studies and protein engineering strategies that have aimed at further improving their catalytic properties or even at turning lipases into acyltransferases. Book Part Antarc* Antarctica antarcticus Unknown 45 68 |
institution |
Open Polar |
collection |
Unknown |
op_collection_id |
ftsupagro |
language |
English |
topic |
Acyltransfer Alcoholysis Biocatalysis CAL-A from Moeziomyces antarcticus (Candida antarctica) CpLIP2 from Candida parapsilosis Structure/function Transesterification aqueous medium acétyltransférase hydrolase milieu aqueux lipase [SDV.IDA]Life Sciences [q-bio]/Food engineering |
spellingShingle |
Acyltransfer Alcoholysis Biocatalysis CAL-A from Moeziomyces antarcticus (Candida antarctica) CpLIP2 from Candida parapsilosis Structure/function Transesterification aqueous medium acétyltransférase hydrolase milieu aqueux lipase [SDV.IDA]Life Sciences [q-bio]/Food engineering Subileau, Maeva Jan, Anne Hélène Dubreucq, Eric Lipases/Acyltransferases for Lipid Modification in Aqueous Media |
topic_facet |
Acyltransfer Alcoholysis Biocatalysis CAL-A from Moeziomyces antarcticus (Candida antarctica) CpLIP2 from Candida parapsilosis Structure/function Transesterification aqueous medium acétyltransférase hydrolase milieu aqueux lipase [SDV.IDA]Life Sciences [q-bio]/Food engineering |
description |
Lipases/acyltransferases are a special group of lipases with highly enhanced affinity for other nucleophiles than water compared to classical lipases. In the presence of a suitable acceptor, they catalyze acyltransfer reactions (such as alcoholysis, aminolysis, or perhydrolysis) much faster than hydrolysis even in aqueous media with a high thermodynamic activity of water (aW). This allows kinetically controlled reactions where the donor ester is fully converted into the ester product before any significant release of free fatty acids. This chapter will discuss the kinetic aspects of the competitive reactions catalyzed by lipases/acyltransferases, propose a model, and a procedure to quantify their acyltransferase character, present some applications, and give an overview of currently known lipases/acyltransferases and of the structural studies and protein engineering strategies that have aimed at further improving their catalytic properties or even at turning lipases into acyltransferases. |
author2 |
Ingénierie des Agro-polymères et Technologies Émergentes (UMR IATE) Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA)-Université Montpellier 2 - Sciences et Techniques (UM2)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Université de Montpellier (UM)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro) |
format |
Book Part |
author |
Subileau, Maeva Jan, Anne Hélène Dubreucq, Eric |
author_facet |
Subileau, Maeva Jan, Anne Hélène Dubreucq, Eric |
author_sort |
Subileau, Maeva |
title |
Lipases/Acyltransferases for Lipid Modification in Aqueous Media |
title_short |
Lipases/Acyltransferases for Lipid Modification in Aqueous Media |
title_full |
Lipases/Acyltransferases for Lipid Modification in Aqueous Media |
title_fullStr |
Lipases/Acyltransferases for Lipid Modification in Aqueous Media |
title_full_unstemmed |
Lipases/Acyltransferases for Lipid Modification in Aqueous Media |
title_sort |
lipases/acyltransferases for lipid modification in aqueous media |
publisher |
HAL CCSD |
publishDate |
2018 |
url |
https://hal.science/hal-01918043 https://doi.org/10.1016/B978-0-12-813167-1.00003-7 |
genre |
Antarc* Antarctica antarcticus |
genre_facet |
Antarc* Antarctica antarcticus |
op_source |
Lipid Modification by Enzymes and Engineered Microbes https://hal.science/hal-01918043 Lipid Modification by Enzymes and Engineered Microbes, Academic Press, 2018, 9780128131671. ⟨10.1016/B978-0-12-813167-1.00003-7⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1016/B978-0-12-813167-1.00003-7 ISBN: 9780128131671 hal-01918043 https://hal.science/hal-01918043 doi:10.1016/B978-0-12-813167-1.00003-7 PRODINRA: 451069 |
op_doi |
https://doi.org/10.1016/B978-0-12-813167-1.00003-7 |
container_start_page |
45 |
op_container_end_page |
68 |
_version_ |
1772820741303042048 |