Major changes in the composition of a Southern Ocean bacterial community in response to diatom-derived dissolved organic matter
International audience In the Southern Ocean, natural iron fertilization in the wake of islands leads to annually occurring spring phytoplankton blooms associated with enhanced heterotrophic activity through the release of labile dissolved organic matter (DOM). The aim of this study was to investiga...
Main Authors: | , , , , , |
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Other Authors: | , , , , , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
HAL CCSD
2018
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Subjects: | |
Online Access: | https://hal.science/hal-01734675 https://hal.science/hal-01734675/document https://hal.science/hal-01734675/file/fiy034.pdf https://doi.org/10.1093/femsec/fiy034 |
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ftsupagro:oai:HAL:hal-01734675v1 |
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record_format |
openpolar |
institution |
Open Polar |
collection |
Portail HAL Institut Agro Montpellier |
op_collection_id |
ftsupagro |
language |
English |
topic |
Dissolved organic matter Southern Ocean Bacterial community composition 16S rRNA gene sequencing Continuous cultures [SDV.BID]Life Sciences [q-bio]/Biodiversity [SDU.OCEAN]Sciences of the Universe [physics]/Ocean Atmosphere [MATH.MATH-DS]Mathematics [math]/Dynamical Systems [math.DS] [INFO.INFO-MO]Computer Science [cs]/Modeling and Simulation |
spellingShingle |
Dissolved organic matter Southern Ocean Bacterial community composition 16S rRNA gene sequencing Continuous cultures [SDV.BID]Life Sciences [q-bio]/Biodiversity [SDU.OCEAN]Sciences of the Universe [physics]/Ocean Atmosphere [MATH.MATH-DS]Mathematics [math]/Dynamical Systems [math.DS] [INFO.INFO-MO]Computer Science [cs]/Modeling and Simulation Landa, Marine Blain, Stéphane Harmand, Jérôme Monchy, Sébastien Rapaport, Alain Obernosterer, Ingrid Major changes in the composition of a Southern Ocean bacterial community in response to diatom-derived dissolved organic matter |
topic_facet |
Dissolved organic matter Southern Ocean Bacterial community composition 16S rRNA gene sequencing Continuous cultures [SDV.BID]Life Sciences [q-bio]/Biodiversity [SDU.OCEAN]Sciences of the Universe [physics]/Ocean Atmosphere [MATH.MATH-DS]Mathematics [math]/Dynamical Systems [math.DS] [INFO.INFO-MO]Computer Science [cs]/Modeling and Simulation |
description |
International audience In the Southern Ocean, natural iron fertilization in the wake of islands leads to annually occurring spring phytoplankton blooms associated with enhanced heterotrophic activity through the release of labile dissolved organic matter (DOM). The aim of this study was to investigate experimentally how diatom-derived DOM affects the composition of Southern Ocean winter water bacterial communities and to identify the most responsive taxa. A bacterial community collected in the naturally iron-fertilized region off Kerguelen Island (KEOPS2 October-November 2011) was grown onboard in continuous cultures, on winter water alone or amended with diatom-derived DOM supplied at identical DOC concentrations. 454 sequencing of 16S amplicons revealed that the two DOM sources sustained strikingly different bacterial communities, with higher relative abundances of Sulfitobacter, Colwellia and Methylophaga OTUs and lower relative abundances of Polaribacter, Marinobacter, NAC11–7 and SAR11 OTUs in diatom-DOM compared to winter water conditions. Using a modeling approach, we obtained growth rates for phylogenetically diverse taxa varying between 0.12 and 0.49 d−1 under carbon-limited conditions. Our results identify diatom-DOM as a key factor shaping Southern Ocean winter water bacterial communities and suggest a role for niche partitioning and microbial interactions in organic matter utilization. |
author2 |
Laboratoire d'Océanographie Microbienne (LOMIC) Observatoire océanologique de Banyuls (OOB) Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) University of California Santa Cruz (UC Santa Cruz) University of California (UC) Laboratoire de Biotechnologie de l'Environnement Narbonne (LBE) Institut National de la Recherche Agronomique (INRA)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro) Laboratoire d’Océanologie et de Géosciences (LOG) - UMR 8187 (LOG) Institut national des sciences de l'Univers (INSU - CNRS)-Université du Littoral Côte d'Opale (ULCO)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD France-Nord ) Mathématiques, Informatique et STatistique pour l'Environnement et l'Agronomie (MISTEA) Centre National de la Recherche Scientifique (CNRS)-Institut National des Sciences de l'Univers (INSU)-Les Enveloppes Fluides et l'Environnement (LEFE)-Cycles Biogeochimiques, Environnement et Ressources (CYBER) French Ministry of Science ANR-10-BLAN-0614,KEOPS 2,Kerguelen : Comparaison plateau Ocean2(2010) |
format |
Article in Journal/Newspaper |
author |
Landa, Marine Blain, Stéphane Harmand, Jérôme Monchy, Sébastien Rapaport, Alain Obernosterer, Ingrid |
author_facet |
Landa, Marine Blain, Stéphane Harmand, Jérôme Monchy, Sébastien Rapaport, Alain Obernosterer, Ingrid |
author_sort |
Landa, Marine |
title |
Major changes in the composition of a Southern Ocean bacterial community in response to diatom-derived dissolved organic matter |
title_short |
Major changes in the composition of a Southern Ocean bacterial community in response to diatom-derived dissolved organic matter |
title_full |
Major changes in the composition of a Southern Ocean bacterial community in response to diatom-derived dissolved organic matter |
title_fullStr |
Major changes in the composition of a Southern Ocean bacterial community in response to diatom-derived dissolved organic matter |
title_full_unstemmed |
Major changes in the composition of a Southern Ocean bacterial community in response to diatom-derived dissolved organic matter |
title_sort |
major changes in the composition of a southern ocean bacterial community in response to diatom-derived dissolved organic matter |
publisher |
HAL CCSD |
publishDate |
2018 |
url |
https://hal.science/hal-01734675 https://hal.science/hal-01734675/document https://hal.science/hal-01734675/file/fiy034.pdf https://doi.org/10.1093/femsec/fiy034 |
long_lat |
ENVELOPE(69.500,69.500,-49.250,-49.250) |
geographic |
Kerguelen Kerguelen Island Southern Ocean |
geographic_facet |
Kerguelen Kerguelen Island Southern Ocean |
genre |
Southern Ocean |
genre_facet |
Southern Ocean |
op_source |
ISSN: 0168-6496 EISSN: 1574-6941 FEMS Microbiology Ecology https://hal.science/hal-01734675 FEMS Microbiology Ecology, 2018, 94 (4), pp.1-46. ⟨10.1093/femsec/fiy034⟩ https://academic.oup.com/femsec/advance-article-abstract/doi/10.1093/femsec/fiy034/4935155?redirectedFrom=fulltext |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1093/femsec/fiy034 info:eu-repo/semantics/altIdentifier/pmid/29547927 hal-01734675 https://hal.science/hal-01734675 https://hal.science/hal-01734675/document https://hal.science/hal-01734675/file/fiy034.pdf doi:10.1093/femsec/fiy034 PRODINRA: 424356 PUBMED: 29547927 WOS: 000429481200014 |
op_rights |
info:eu-repo/semantics/OpenAccess |
op_doi |
https://doi.org/10.1093/femsec/fiy03410.1093/femsec/fiy034/4935155 |
_version_ |
1802650277606588416 |
spelling |
ftsupagro:oai:HAL:hal-01734675v1 2024-06-23T07:56:54+00:00 Major changes in the composition of a Southern Ocean bacterial community in response to diatom-derived dissolved organic matter Landa, Marine Blain, Stéphane Harmand, Jérôme Monchy, Sébastien Rapaport, Alain Obernosterer, Ingrid Laboratoire d'Océanographie Microbienne (LOMIC) Observatoire océanologique de Banyuls (OOB) Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) University of California Santa Cruz (UC Santa Cruz) University of California (UC) Laboratoire de Biotechnologie de l'Environnement Narbonne (LBE) Institut National de la Recherche Agronomique (INRA)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro) Laboratoire d’Océanologie et de Géosciences (LOG) - UMR 8187 (LOG) Institut national des sciences de l'Univers (INSU - CNRS)-Université du Littoral Côte d'Opale (ULCO)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD France-Nord ) Mathématiques, Informatique et STatistique pour l'Environnement et l'Agronomie (MISTEA) Centre National de la Recherche Scientifique (CNRS)-Institut National des Sciences de l'Univers (INSU)-Les Enveloppes Fluides et l'Environnement (LEFE)-Cycles Biogeochimiques, Environnement et Ressources (CYBER) French Ministry of Science ANR-10-BLAN-0614,KEOPS 2,Kerguelen : Comparaison plateau Ocean2(2010) 2018-03-14 https://hal.science/hal-01734675 https://hal.science/hal-01734675/document https://hal.science/hal-01734675/file/fiy034.pdf https://doi.org/10.1093/femsec/fiy034 en eng HAL CCSD Wiley-Blackwell info:eu-repo/semantics/altIdentifier/doi/10.1093/femsec/fiy034 info:eu-repo/semantics/altIdentifier/pmid/29547927 hal-01734675 https://hal.science/hal-01734675 https://hal.science/hal-01734675/document https://hal.science/hal-01734675/file/fiy034.pdf doi:10.1093/femsec/fiy034 PRODINRA: 424356 PUBMED: 29547927 WOS: 000429481200014 info:eu-repo/semantics/OpenAccess ISSN: 0168-6496 EISSN: 1574-6941 FEMS Microbiology Ecology https://hal.science/hal-01734675 FEMS Microbiology Ecology, 2018, 94 (4), pp.1-46. ⟨10.1093/femsec/fiy034⟩ https://academic.oup.com/femsec/advance-article-abstract/doi/10.1093/femsec/fiy034/4935155?redirectedFrom=fulltext Dissolved organic matter Southern Ocean Bacterial community composition 16S rRNA gene sequencing Continuous cultures [SDV.BID]Life Sciences [q-bio]/Biodiversity [SDU.OCEAN]Sciences of the Universe [physics]/Ocean Atmosphere [MATH.MATH-DS]Mathematics [math]/Dynamical Systems [math.DS] [INFO.INFO-MO]Computer Science [cs]/Modeling and Simulation info:eu-repo/semantics/article Journal articles 2018 ftsupagro https://doi.org/10.1093/femsec/fiy03410.1093/femsec/fiy034/4935155 2024-06-05T23:36:09Z International audience In the Southern Ocean, natural iron fertilization in the wake of islands leads to annually occurring spring phytoplankton blooms associated with enhanced heterotrophic activity through the release of labile dissolved organic matter (DOM). The aim of this study was to investigate experimentally how diatom-derived DOM affects the composition of Southern Ocean winter water bacterial communities and to identify the most responsive taxa. A bacterial community collected in the naturally iron-fertilized region off Kerguelen Island (KEOPS2 October-November 2011) was grown onboard in continuous cultures, on winter water alone or amended with diatom-derived DOM supplied at identical DOC concentrations. 454 sequencing of 16S amplicons revealed that the two DOM sources sustained strikingly different bacterial communities, with higher relative abundances of Sulfitobacter, Colwellia and Methylophaga OTUs and lower relative abundances of Polaribacter, Marinobacter, NAC11–7 and SAR11 OTUs in diatom-DOM compared to winter water conditions. Using a modeling approach, we obtained growth rates for phylogenetically diverse taxa varying between 0.12 and 0.49 d−1 under carbon-limited conditions. Our results identify diatom-DOM as a key factor shaping Southern Ocean winter water bacterial communities and suggest a role for niche partitioning and microbial interactions in organic matter utilization. Article in Journal/Newspaper Southern Ocean Portail HAL Institut Agro Montpellier Kerguelen Kerguelen Island ENVELOPE(69.500,69.500,-49.250,-49.250) Southern Ocean |