Down in the pond: Isolation and characterization of a new Serratia marcescens strain (LVF3) from the surface water near frog’s lettuce (Groenlandia densa)
Serratia marcescens is a species that belongs to the family of Yersiniaceae . This family comprises taxa representing opportunistic human- and phytopathogens but also plant growth-promoting rhizobacteria (PGPR). This study describes a novel Gram-negative strain (LVF3 R ) of the species Serratia marc...
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ftsubgoettingen:oai:publications.goettingen-research-online.de:2/94424 2023-09-05T13:19:59+02:00 Down in the pond: Isolation and characterization of a new Serratia marcescens strain (LVF3) from the surface water near frog’s lettuce (Groenlandia densa) Friedrich, Ines Bodenberger, Bernhard Neubauer, Hannes Hertel, Robert Daniel, Rolf 2021 https://resolver.sub.uni-goettingen.de/purl?gro-2/94424 https://doi.org/10.1371/journal.pone.0259673 en eng 1932-6203 https://resolver.sub.uni-goettingen.de/purl?gro-2/94424 doi:10.1371/journal.pone.0259673 CC BY 4.0 journal_article original_ja yes published_version 2021 ftsubgoettingen https://doi.org/10.1371/journal.pone.0259673 2023-08-20T22:16:04Z Serratia marcescens is a species that belongs to the family of Yersiniaceae . This family comprises taxa representing opportunistic human- and phytopathogens but also plant growth-promoting rhizobacteria (PGPR). This study describes a novel Gram-negative strain (LVF3 R ) of the species Serratia marcescens . The strain was characterized genomically, morphologically, and physiologically. In addition, the potential of the isolate to act as a host strain to assess the diversity of Serratia associated phages in environmental samples was explored. Average nucleotide identity analysis revealed that LVF3 R belongs to the species Serratia marcescens . In silico analysis and ProphageSeq data resulted in the identification of one prophage, which is capable of viral particle formation. Electron microscopy showed cells of a rod-shaped, flagellated morphotype. The cells revealed a length and width of 1–1.6 μm and 0.8 μm, respectively. LVF3 R showed optimal growth at 30 C and in the presence of up to 2% (w/v) NaCl. It exhibited resistances to ampicillin, erythromycin, oxacillin, oxytetracycline, rifampicin, tetracycline, and vancomycin. Genome data indicate that strain S . marcescens LVF3 R is a potential PGPR strain. It harbors genes coding for indole acetic acid ( IAA) biosynthesis, siderophore production, plant polymer degradation enzymes, acetoin synthesis, flagellar proteins, type IV secretion system, chemotaxis, phosphorous solubilization, and biofilm formation. Open-Access-Publikationsfonds 2021 Article in Journal/Newspaper Groenlandia Georg-August-Universität Göttingen: GoeScholar PLOS ONE 16 11 e0259673 |
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Open Polar |
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Georg-August-Universität Göttingen: GoeScholar |
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ftsubgoettingen |
language |
English |
description |
Serratia marcescens is a species that belongs to the family of Yersiniaceae . This family comprises taxa representing opportunistic human- and phytopathogens but also plant growth-promoting rhizobacteria (PGPR). This study describes a novel Gram-negative strain (LVF3 R ) of the species Serratia marcescens . The strain was characterized genomically, morphologically, and physiologically. In addition, the potential of the isolate to act as a host strain to assess the diversity of Serratia associated phages in environmental samples was explored. Average nucleotide identity analysis revealed that LVF3 R belongs to the species Serratia marcescens . In silico analysis and ProphageSeq data resulted in the identification of one prophage, which is capable of viral particle formation. Electron microscopy showed cells of a rod-shaped, flagellated morphotype. The cells revealed a length and width of 1–1.6 μm and 0.8 μm, respectively. LVF3 R showed optimal growth at 30 C and in the presence of up to 2% (w/v) NaCl. It exhibited resistances to ampicillin, erythromycin, oxacillin, oxytetracycline, rifampicin, tetracycline, and vancomycin. Genome data indicate that strain S . marcescens LVF3 R is a potential PGPR strain. It harbors genes coding for indole acetic acid ( IAA) biosynthesis, siderophore production, plant polymer degradation enzymes, acetoin synthesis, flagellar proteins, type IV secretion system, chemotaxis, phosphorous solubilization, and biofilm formation. Open-Access-Publikationsfonds 2021 |
format |
Article in Journal/Newspaper |
author |
Friedrich, Ines Bodenberger, Bernhard Neubauer, Hannes Hertel, Robert Daniel, Rolf |
spellingShingle |
Friedrich, Ines Bodenberger, Bernhard Neubauer, Hannes Hertel, Robert Daniel, Rolf Down in the pond: Isolation and characterization of a new Serratia marcescens strain (LVF3) from the surface water near frog’s lettuce (Groenlandia densa) |
author_facet |
Friedrich, Ines Bodenberger, Bernhard Neubauer, Hannes Hertel, Robert Daniel, Rolf |
author_sort |
Friedrich, Ines |
title |
Down in the pond: Isolation and characterization of a new Serratia marcescens strain (LVF3) from the surface water near frog’s lettuce (Groenlandia densa) |
title_short |
Down in the pond: Isolation and characterization of a new Serratia marcescens strain (LVF3) from the surface water near frog’s lettuce (Groenlandia densa) |
title_full |
Down in the pond: Isolation and characterization of a new Serratia marcescens strain (LVF3) from the surface water near frog’s lettuce (Groenlandia densa) |
title_fullStr |
Down in the pond: Isolation and characterization of a new Serratia marcescens strain (LVF3) from the surface water near frog’s lettuce (Groenlandia densa) |
title_full_unstemmed |
Down in the pond: Isolation and characterization of a new Serratia marcescens strain (LVF3) from the surface water near frog’s lettuce (Groenlandia densa) |
title_sort |
down in the pond: isolation and characterization of a new serratia marcescens strain (lvf3) from the surface water near frog’s lettuce (groenlandia densa) |
publishDate |
2021 |
url |
https://resolver.sub.uni-goettingen.de/purl?gro-2/94424 https://doi.org/10.1371/journal.pone.0259673 |
genre |
Groenlandia |
genre_facet |
Groenlandia |
op_relation |
1932-6203 https://resolver.sub.uni-goettingen.de/purl?gro-2/94424 doi:10.1371/journal.pone.0259673 |
op_rights |
CC BY 4.0 |
op_doi |
https://doi.org/10.1371/journal.pone.0259673 |
container_title |
PLOS ONE |
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16 |
container_issue |
11 |
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e0259673 |
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1776200737222557696 |