Microbial Diversity and Biochemical Potential Encoded by Thermal Spring Metagenomes Derived from the Kamchatka Peninsula
Volcanic regions contain a variety of environments suitable for extremophiles. This study was focused on assessing and exploiting the prokaryotic diversity of two microbial communities derived from different Kamchatkian thermal springs by metagenomic approaches. Samples were taken from a thermoacido...
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ftsubgoettingen:oai:publications.goettingen-research-online.de:2/31245 2023-09-05T13:20:46+02:00 Microbial Diversity and Biochemical Potential Encoded by Thermal Spring Metagenomes Derived from the Kamchatka Peninsula Wemheuer, Bernd Taube, Robert Akyol, Pinar Wemheuer, Franziska Daniel, Rolf 2013 https://resolver.sub.uni-goettingen.de/purl?gro-2/31245 https://resolver.sub.uni-goettingen.de/purl?gs-1/9968 https://doi.org/10.1155/2013/136714 unknown 1472-3646 https://resolver.sub.uni-goettingen.de/purl?gro-2/31245 doi:10.1155/2013/136714 000315875000001 https://resolver.sub.uni-goettingen.de/purl?gs-1/9968 journal_article yes published_version 2013 ftsubgoettingen https://doi.org/10.1155/2013/136714 2023-08-20T22:13:08Z Volcanic regions contain a variety of environments suitable for extremophiles. This study was focused on assessing and exploiting the prokaryotic diversity of two microbial communities derived from different Kamchatkian thermal springs by metagenomic approaches. Samples were taken from a thermoacidophilic spring near the Mutnovsky Volcano and from a thermophilic spring in the Uzon Caldera. Environmental DNA for metagenomic analysis was isolated from collected sediment samples by direct cell lysis. The prokaryotic community composition was examined by analysis of archaeal and bacterial 16S rRNA genes. A total number of 1235 16S rRNA gene sequences were obtained and used for taxonomic classification. Most abundant in the samples were members of Thaumarchaeota, Thermotogae, and Proteobacteria. The Mutnovsky hot spring was dominated by the Terrestrial Hot Spring Group, Kosmotoga, and Acidithiobacillus. The Uzon Caldera was dominated by uncultured members of the Miscellaneous Crenarchaeotic Group and Enterobacteriaceae. The remaining 16S rRNA gene sequences belonged to the Aquificae, Dictyoglomi, Euryarchaeota, Korarchaeota, Thermodesulfobacteria, Firmicutes, and some potential new phyla. In addition, the recovered DNA was used for generation of metagenomic libraries, which were subsequently mined for genes encoding lipolytic and proteolytic enzymes. Three novel genes conferring lipolytic and one gene conferring proteolytic activity were identified. Bundesministerium fur Bildung und Forschung (BMBF) Article in Journal/Newspaper Kamchatka Kamchatka Peninsula Georg-August-Universität Göttingen: GoeScholar Kamchatka Peninsula ENVELOPE(160.000,160.000,56.000,56.000) Archaea 2013 1 13 |
institution |
Open Polar |
collection |
Georg-August-Universität Göttingen: GoeScholar |
op_collection_id |
ftsubgoettingen |
language |
unknown |
description |
Volcanic regions contain a variety of environments suitable for extremophiles. This study was focused on assessing and exploiting the prokaryotic diversity of two microbial communities derived from different Kamchatkian thermal springs by metagenomic approaches. Samples were taken from a thermoacidophilic spring near the Mutnovsky Volcano and from a thermophilic spring in the Uzon Caldera. Environmental DNA for metagenomic analysis was isolated from collected sediment samples by direct cell lysis. The prokaryotic community composition was examined by analysis of archaeal and bacterial 16S rRNA genes. A total number of 1235 16S rRNA gene sequences were obtained and used for taxonomic classification. Most abundant in the samples were members of Thaumarchaeota, Thermotogae, and Proteobacteria. The Mutnovsky hot spring was dominated by the Terrestrial Hot Spring Group, Kosmotoga, and Acidithiobacillus. The Uzon Caldera was dominated by uncultured members of the Miscellaneous Crenarchaeotic Group and Enterobacteriaceae. The remaining 16S rRNA gene sequences belonged to the Aquificae, Dictyoglomi, Euryarchaeota, Korarchaeota, Thermodesulfobacteria, Firmicutes, and some potential new phyla. In addition, the recovered DNA was used for generation of metagenomic libraries, which were subsequently mined for genes encoding lipolytic and proteolytic enzymes. Three novel genes conferring lipolytic and one gene conferring proteolytic activity were identified. Bundesministerium fur Bildung und Forschung (BMBF) |
format |
Article in Journal/Newspaper |
author |
Wemheuer, Bernd Taube, Robert Akyol, Pinar Wemheuer, Franziska Daniel, Rolf |
spellingShingle |
Wemheuer, Bernd Taube, Robert Akyol, Pinar Wemheuer, Franziska Daniel, Rolf Microbial Diversity and Biochemical Potential Encoded by Thermal Spring Metagenomes Derived from the Kamchatka Peninsula |
author_facet |
Wemheuer, Bernd Taube, Robert Akyol, Pinar Wemheuer, Franziska Daniel, Rolf |
author_sort |
Wemheuer, Bernd |
title |
Microbial Diversity and Biochemical Potential Encoded by Thermal Spring Metagenomes Derived from the Kamchatka Peninsula |
title_short |
Microbial Diversity and Biochemical Potential Encoded by Thermal Spring Metagenomes Derived from the Kamchatka Peninsula |
title_full |
Microbial Diversity and Biochemical Potential Encoded by Thermal Spring Metagenomes Derived from the Kamchatka Peninsula |
title_fullStr |
Microbial Diversity and Biochemical Potential Encoded by Thermal Spring Metagenomes Derived from the Kamchatka Peninsula |
title_full_unstemmed |
Microbial Diversity and Biochemical Potential Encoded by Thermal Spring Metagenomes Derived from the Kamchatka Peninsula |
title_sort |
microbial diversity and biochemical potential encoded by thermal spring metagenomes derived from the kamchatka peninsula |
publishDate |
2013 |
url |
https://resolver.sub.uni-goettingen.de/purl?gro-2/31245 https://resolver.sub.uni-goettingen.de/purl?gs-1/9968 https://doi.org/10.1155/2013/136714 |
long_lat |
ENVELOPE(160.000,160.000,56.000,56.000) |
geographic |
Kamchatka Peninsula |
geographic_facet |
Kamchatka Peninsula |
genre |
Kamchatka Kamchatka Peninsula |
genre_facet |
Kamchatka Kamchatka Peninsula |
op_relation |
1472-3646 https://resolver.sub.uni-goettingen.de/purl?gro-2/31245 doi:10.1155/2013/136714 000315875000001 https://resolver.sub.uni-goettingen.de/purl?gs-1/9968 |
op_doi |
https://doi.org/10.1155/2013/136714 |
container_title |
Archaea |
container_volume |
2013 |
container_start_page |
1 |
op_container_end_page |
13 |
_version_ |
1776201399740137472 |