Distribution of EP4 receptor in different Atlantic salmon (Salmo salar L.) tissues.
Prostaglandin E2 (PGE2) is an important lipid mediator that plays diverse functions in mammals. Four receptor subtypes of PGE2, designated EP1-4, have been identified to mediate its signaling pathways. Extensive studies of PGE2 and its receptors have been carried out in mammals, but little is known...
Published in: | Developmental & Comparative Immunology |
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Online Access: | http://resolver.sub.uni-goettingen.de/purl?gs-1/11232 https://doi.org/10.1016/j.dci.2014.09.013 |
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ftsubgoettingen:oai:goescholar:1/11232 2023-05-15T15:28:27+02:00 Distribution of EP4 receptor in different Atlantic salmon (Salmo salar L.) tissues. Gamil, A. A. A. Guo, T.-C. König, M. Evensen, O. 2015 http://resolver.sub.uni-goettingen.de/purl?gs-1/11232 https://doi.org/10.1016/j.dci.2014.09.013 eng eng 1879-0089 Gamil, A A A; Guo, T-C; König, M; Evensen, O (2015): Distribution of EP4 receptor in different Atlantic salmon (Salmo salar L.) tissues. - Developmental and comparative immunology, Vol. 48, Nr. 1, p. 143-50 http://resolver.sub.uni-goettingen.de/purl?gs-1/11232 doi:10.1016/j.dci.2014.09.013 25307202 openAccess http://creativecommons.org/licenses/by-nc-nd/3.0/ CC-BY-NC-ND Atlantic salmon EP4 Tissue distribution mRNA Immunohistochemistry Immune organs journalArticle publishedVersion 2015 ftsubgoettingen https://doi.org/10.1016/j.dci.2014.09.013 2022-11-02T09:28:25Z Prostaglandin E2 (PGE2) is an important lipid mediator that plays diverse functions in mammals. Four receptor subtypes of PGE2, designated EP1-4, have been identified to mediate its signaling pathways. Extensive studies of PGE2 and its receptors have been carried out in mammals, but little is known in fish, including Atlantic salmon. In the current study, the distribution of Atlantic salmon EP4 receptor in different tissues was investigated using RT- and real-time PCR. A custom made antibody was used to investigate the distribution of this receptor in different tissues. Quantitative analysis by real-time PCR revealed that the expression was more abundant in the spleen followed by head kidney, skin and fin while it was least expressed in heart, muscles and brain. The staining intensity obtained by immunohistochemistry correlated with the RT-PCR results. EP4 expression was strongly associated with the immune cells in different tissues. To our knowledge, this is the first study to describe the distribution of EP4 receptor in Atlantic salmon tissues. Our findings suggest that EP4 may play a role in mediating immune responses as observed in mammals. peerReviewed Article in Journal/Newspaper Atlantic salmon Salmo salar Georg-August-Universität Göttingen: GoeScholar Developmental & Comparative Immunology 48 1 143 150 |
institution |
Open Polar |
collection |
Georg-August-Universität Göttingen: GoeScholar |
op_collection_id |
ftsubgoettingen |
language |
English |
topic |
Atlantic salmon EP4 Tissue distribution mRNA Immunohistochemistry Immune organs |
spellingShingle |
Atlantic salmon EP4 Tissue distribution mRNA Immunohistochemistry Immune organs Gamil, A. A. A. Guo, T.-C. König, M. Evensen, O. Distribution of EP4 receptor in different Atlantic salmon (Salmo salar L.) tissues. |
topic_facet |
Atlantic salmon EP4 Tissue distribution mRNA Immunohistochemistry Immune organs |
description |
Prostaglandin E2 (PGE2) is an important lipid mediator that plays diverse functions in mammals. Four receptor subtypes of PGE2, designated EP1-4, have been identified to mediate its signaling pathways. Extensive studies of PGE2 and its receptors have been carried out in mammals, but little is known in fish, including Atlantic salmon. In the current study, the distribution of Atlantic salmon EP4 receptor in different tissues was investigated using RT- and real-time PCR. A custom made antibody was used to investigate the distribution of this receptor in different tissues. Quantitative analysis by real-time PCR revealed that the expression was more abundant in the spleen followed by head kidney, skin and fin while it was least expressed in heart, muscles and brain. The staining intensity obtained by immunohistochemistry correlated with the RT-PCR results. EP4 expression was strongly associated with the immune cells in different tissues. To our knowledge, this is the first study to describe the distribution of EP4 receptor in Atlantic salmon tissues. Our findings suggest that EP4 may play a role in mediating immune responses as observed in mammals. peerReviewed |
format |
Article in Journal/Newspaper |
author |
Gamil, A. A. A. Guo, T.-C. König, M. Evensen, O. |
author_facet |
Gamil, A. A. A. Guo, T.-C. König, M. Evensen, O. |
author_sort |
Gamil, A. A. A. |
title |
Distribution of EP4 receptor in different Atlantic salmon (Salmo salar L.) tissues. |
title_short |
Distribution of EP4 receptor in different Atlantic salmon (Salmo salar L.) tissues. |
title_full |
Distribution of EP4 receptor in different Atlantic salmon (Salmo salar L.) tissues. |
title_fullStr |
Distribution of EP4 receptor in different Atlantic salmon (Salmo salar L.) tissues. |
title_full_unstemmed |
Distribution of EP4 receptor in different Atlantic salmon (Salmo salar L.) tissues. |
title_sort |
distribution of ep4 receptor in different atlantic salmon (salmo salar l.) tissues. |
publishDate |
2015 |
url |
http://resolver.sub.uni-goettingen.de/purl?gs-1/11232 https://doi.org/10.1016/j.dci.2014.09.013 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_relation |
1879-0089 Gamil, A A A; Guo, T-C; König, M; Evensen, O (2015): Distribution of EP4 receptor in different Atlantic salmon (Salmo salar L.) tissues. - Developmental and comparative immunology, Vol. 48, Nr. 1, p. 143-50 http://resolver.sub.uni-goettingen.de/purl?gs-1/11232 doi:10.1016/j.dci.2014.09.013 25307202 |
op_rights |
openAccess http://creativecommons.org/licenses/by-nc-nd/3.0/ |
op_rightsnorm |
CC-BY-NC-ND |
op_doi |
https://doi.org/10.1016/j.dci.2014.09.013 |
container_title |
Developmental & Comparative Immunology |
container_volume |
48 |
container_issue |
1 |
container_start_page |
143 |
op_container_end_page |
150 |
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1766358813586227200 |