Stadien-abhängiger Nachweis von CD14+- und CD16+-Zellen im humanen Herz- und Milzgewebe nach Myokardinfarkt: Eine post-mortem-Analyse
Monocytes play an important role in cardiovascular wound healing processes. Human monocytes can be classified into two subsets based on the expression of CD14 and CD16. Here, the temporal and spatial distribution of CD14⁺ and CD16⁺ cells after myocardial infarction (MI) in human heart and spleen tis...
Main Author: | |
---|---|
Other Authors: | , , |
Format: | Doctoral or Postdoctoral Thesis |
Language: | German |
Published: |
2014
|
Subjects: | |
Online Access: | http://hdl.handle.net/11858/00-1735-0000-0022-5F05-E https://doi.org/10.53846/goediss-4581 https://nbn-resolving.org/urn:nbn:de:gbv:7-11858/00-1735-0000-0022-5F05-E-5 |
id |
ftsubgoettdiss:oai:ediss.uni-goettingen.de:11858/00-1735-0000-0022-5F05-E |
---|---|
record_format |
openpolar |
spelling |
ftsubgoettdiss:oai:ediss.uni-goettingen.de:11858/00-1735-0000-0022-5F05-E 2023-09-05T13:22:54+02:00 Stadien-abhängiger Nachweis von CD14+- und CD16+-Zellen im humanen Herz- und Milzgewebe nach Myokardinfarkt: Eine post-mortem-Analyse Stage-dependent detection of CD14+ and CD16+ immune cells in human heart tissue after myocardial infarction: A post-mortem analysis Schlegel, Magdalena Schäfer, Katrin Prof. Dr. Schön, Margarete Prof. Dr. Dressel, Ralf Prof. Dr. 2014-07-02 http://hdl.handle.net/11858/00-1735-0000-0022-5F05-E https://doi.org/10.53846/goediss-4581 https://nbn-resolving.org/urn:nbn:de:gbv:7-11858/00-1735-0000-0022-5F05-E-5 deu ger http://hdl.handle.net/11858/00-1735-0000-0022-5F05-E http://dx.doi.org/10.53846/goediss-4581 urn:nbn:de:gbv:7-11858/00-1735-0000-0022-5F05-E-5 789515695 http://creativecommons.org/licenses/by-nc-nd/3.0/ 610 Monocytes CD14 CD16 Myocardial infarction Osteopontin KLF4 Kardiologie (PPN619875755) doctoralThesis 2014 ftsubgoettdiss https://doi.org/10.53846/goediss-4581 2023-08-18T08:30:44Z Monocytes play an important role in cardiovascular wound healing processes. Human monocytes can be classified into two subsets based on the expression of CD14 and CD16. Here, the temporal and spatial distribution of CD14⁺ and CD16⁺ cells after myocardial infarction (MI) in human heart and spleen tissue was examined and correlated with markers of cardiac repair. Heart samples obtained at autopsy were histologically classified into acute (AMI; n = 11), subacute (SAMI; n = 10) and old (OMI; n = 16) MI, or control myocardium (CONTR; n = 8). Histochemical analyses of heart tissue revealed an increase of CD14⁺ and CD16⁺ cells in subjects with SAMI. Regarding a MI-associated release of monocytes from spleen reservoirs, a non-significant reduction of splenic CD14⁺ and CD16⁺ cells was detected in subjects with AMI with an increased number of CD14⁺, CD16⁺ and Ki67⁺ cells in SAMI. The hypoxia indicator carbonic anhydrase IX was predominantly expressed in AMI (p < 0.01 vs. OMI and CONTR) and SAMI (p < 0.05 vs. OMI and CONTR). The number of capillaries was increased in AMI (p<0,05 vs. OMI), showing that hypoxia is a strong trigger off angiogenesis. Marked fibrosis could be observed in OMI (p<0,001 vs. CONTR). The adhesion molecule CD56 was also strongly expressed in OMI (p < 0.01 vs. CONTR) and found to correlate with fibrosis (p < 0.001). The monocyte chemoattractrant osteopontin was more highly expressed in hearts of SAMI patients (p < 0.01 vs. CONTR). KLF4⁺ cells were also increased in SAMI (p<0,001 vs. OMI), which could indicate a switch between the inflammatory and reparative phase of MI by influencing the polarization of monocytes. In conclusion, disease stage-specific alterations in CD14⁺ and CD16⁺ cells in human heart may contribute to cardiac repair processes following MI. 2014-07-30 Doctoral or Postdoctoral Thesis sami Georg-August-Universität Göttingen: eDiss |
institution |
Open Polar |
collection |
Georg-August-Universität Göttingen: eDiss |
op_collection_id |
ftsubgoettdiss |
language |
German |
topic |
610 Monocytes CD14 CD16 Myocardial infarction Osteopontin KLF4 Kardiologie (PPN619875755) |
spellingShingle |
610 Monocytes CD14 CD16 Myocardial infarction Osteopontin KLF4 Kardiologie (PPN619875755) Schlegel, Magdalena Stadien-abhängiger Nachweis von CD14+- und CD16+-Zellen im humanen Herz- und Milzgewebe nach Myokardinfarkt: Eine post-mortem-Analyse |
topic_facet |
610 Monocytes CD14 CD16 Myocardial infarction Osteopontin KLF4 Kardiologie (PPN619875755) |
description |
Monocytes play an important role in cardiovascular wound healing processes. Human monocytes can be classified into two subsets based on the expression of CD14 and CD16. Here, the temporal and spatial distribution of CD14⁺ and CD16⁺ cells after myocardial infarction (MI) in human heart and spleen tissue was examined and correlated with markers of cardiac repair. Heart samples obtained at autopsy were histologically classified into acute (AMI; n = 11), subacute (SAMI; n = 10) and old (OMI; n = 16) MI, or control myocardium (CONTR; n = 8). Histochemical analyses of heart tissue revealed an increase of CD14⁺ and CD16⁺ cells in subjects with SAMI. Regarding a MI-associated release of monocytes from spleen reservoirs, a non-significant reduction of splenic CD14⁺ and CD16⁺ cells was detected in subjects with AMI with an increased number of CD14⁺, CD16⁺ and Ki67⁺ cells in SAMI. The hypoxia indicator carbonic anhydrase IX was predominantly expressed in AMI (p < 0.01 vs. OMI and CONTR) and SAMI (p < 0.05 vs. OMI and CONTR). The number of capillaries was increased in AMI (p<0,05 vs. OMI), showing that hypoxia is a strong trigger off angiogenesis. Marked fibrosis could be observed in OMI (p<0,001 vs. CONTR). The adhesion molecule CD56 was also strongly expressed in OMI (p < 0.01 vs. CONTR) and found to correlate with fibrosis (p < 0.001). The monocyte chemoattractrant osteopontin was more highly expressed in hearts of SAMI patients (p < 0.01 vs. CONTR). KLF4⁺ cells were also increased in SAMI (p<0,001 vs. OMI), which could indicate a switch between the inflammatory and reparative phase of MI by influencing the polarization of monocytes. In conclusion, disease stage-specific alterations in CD14⁺ and CD16⁺ cells in human heart may contribute to cardiac repair processes following MI. 2014-07-30 |
author2 |
Schäfer, Katrin Prof. Dr. Schön, Margarete Prof. Dr. Dressel, Ralf Prof. Dr. |
format |
Doctoral or Postdoctoral Thesis |
author |
Schlegel, Magdalena |
author_facet |
Schlegel, Magdalena |
author_sort |
Schlegel, Magdalena |
title |
Stadien-abhängiger Nachweis von CD14+- und CD16+-Zellen im humanen Herz- und Milzgewebe nach Myokardinfarkt: Eine post-mortem-Analyse |
title_short |
Stadien-abhängiger Nachweis von CD14+- und CD16+-Zellen im humanen Herz- und Milzgewebe nach Myokardinfarkt: Eine post-mortem-Analyse |
title_full |
Stadien-abhängiger Nachweis von CD14+- und CD16+-Zellen im humanen Herz- und Milzgewebe nach Myokardinfarkt: Eine post-mortem-Analyse |
title_fullStr |
Stadien-abhängiger Nachweis von CD14+- und CD16+-Zellen im humanen Herz- und Milzgewebe nach Myokardinfarkt: Eine post-mortem-Analyse |
title_full_unstemmed |
Stadien-abhängiger Nachweis von CD14+- und CD16+-Zellen im humanen Herz- und Milzgewebe nach Myokardinfarkt: Eine post-mortem-Analyse |
title_sort |
stadien-abhängiger nachweis von cd14+- und cd16+-zellen im humanen herz- und milzgewebe nach myokardinfarkt: eine post-mortem-analyse |
publishDate |
2014 |
url |
http://hdl.handle.net/11858/00-1735-0000-0022-5F05-E https://doi.org/10.53846/goediss-4581 https://nbn-resolving.org/urn:nbn:de:gbv:7-11858/00-1735-0000-0022-5F05-E-5 |
genre |
sami |
genre_facet |
sami |
op_relation |
http://hdl.handle.net/11858/00-1735-0000-0022-5F05-E http://dx.doi.org/10.53846/goediss-4581 urn:nbn:de:gbv:7-11858/00-1735-0000-0022-5F05-E-5 789515695 |
op_rights |
http://creativecommons.org/licenses/by-nc-nd/3.0/ |
op_doi |
https://doi.org/10.53846/goediss-4581 |
_version_ |
1776203458728165376 |