Blue Blood on Ice : Cephalopod haemocyanin function and evolution in a latitudinal cline
The Southern Ocean hosts a rich and diverse fauna that required specialist adaptations to colonize and persist at temperatures close to freezing. While much has been revealed about key adaptations in Antarctic fishes little is known about evolutionary strategies of other Antarctic ectotherms, partic...
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Universität Bremen
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ftsubbremen:oai:media.suub.uni-bremen.de:Publications/elib/823 2023-05-15T14:04:15+02:00 Blue Blood on Ice : Cephalopod haemocyanin function and evolution in a latitudinal cline Blaues Blut unterm Eis : Funktion und Evolution von Cephalopod Haemocyanin entlang latitudinaler Gradienten. Oellermann, Michael Pörtner, Hans Otto Lieb, Bernhard 2015-02-04 application/pdf https://media.suub.uni-bremen.de/handle/elib/823 https://nbn-resolving.org/urn:nbn:de:gbv:46-00104376-17 eng eng Universität Bremen FB2 Biologie/Chemie https://media.suub.uni-bremen.de/handle/elib/823 urn:nbn:de:gbv:46-00104376-17 info:eu-repo/semantics/openAccess Oxygen equilibrium curve haemocyanin hemocyanin cephalopod oxygen affinity diffusion chamber octopod cold tolerance positive selection 590 590 Animals (Zoology) ddc:590 Dissertation doctoralThesis 2015 ftsubbremen 2022-11-09T07:09:27Z The Southern Ocean hosts a rich and diverse fauna that required specialist adaptations to colonize and persist at temperatures close to freezing. While much has been revealed about key adaptations in Antarctic fishes little is known about evolutionary strategies of other Antarctic ectotherms, particularly the abundant benthic incirrate octopods. Their oxygen demand is largely fuelled by the blue oxygen transporter haemocyanin that however, due to its increasing functional failure towards colder temperatures, poses a prime target for cold-adaptive adjustment. While haemocyanin structure has been well understood it remains unclear which molecular features and evolutionary trajectories explain functional properties of octopod haemocyanin. This thesis thus aimed to unravel cold-adaptive features of octopod haemocyanin and the underlying molecular features that evolved to sustain oxygen supply at sub-zero temperatures. Haemocyanin function is best assessed by oxygen binding experiments, which however was challenged due to the minute haemolymph volumes non-model organisms like Antarctic octopods, yield. I thus upgraded an oxygen diffusion chamber with a broad range fibre optic spectrophotometer and a micro-pH optode and tested the setup for Octopus vulgaris, the Antarctic eelpout and a Baikal amphipod. This technical advancement enabled simultaneous recordings of pH and oxygen dependent pigment absorbance in only 15 microliter of sample. Results were highly reproducible and accurate and provided detailed insight to the complex and dynamic spectral features of three diverse blood-types. To identify cold-adaptive functional traits of blood oxygen transport this study compared haemocyanin oxygen binding properties, oxygen carrying capacities and haemolymph protein and ion composition between the Antarctic octopod Pareledone charcoti, the temperate Octopus pallidus and the subtropical Eledone moschata. Compared to octopods from warmer climates, Pareledone charcoti showed incomplete but significantly reduced oxygen ... Doctoral or Postdoctoral Thesis Antarc* Antarctic Southern Ocean Media SuUB Bremen (Staats- und Universitätsbibliothek Bremen) Antarctic Southern Ocean The Antarctic |
institution |
Open Polar |
collection |
Media SuUB Bremen (Staats- und Universitätsbibliothek Bremen) |
op_collection_id |
ftsubbremen |
language |
English |
topic |
Oxygen equilibrium curve haemocyanin hemocyanin cephalopod oxygen affinity diffusion chamber octopod cold tolerance positive selection 590 590 Animals (Zoology) ddc:590 |
spellingShingle |
Oxygen equilibrium curve haemocyanin hemocyanin cephalopod oxygen affinity diffusion chamber octopod cold tolerance positive selection 590 590 Animals (Zoology) ddc:590 Oellermann, Michael Blue Blood on Ice : Cephalopod haemocyanin function and evolution in a latitudinal cline |
topic_facet |
Oxygen equilibrium curve haemocyanin hemocyanin cephalopod oxygen affinity diffusion chamber octopod cold tolerance positive selection 590 590 Animals (Zoology) ddc:590 |
description |
The Southern Ocean hosts a rich and diverse fauna that required specialist adaptations to colonize and persist at temperatures close to freezing. While much has been revealed about key adaptations in Antarctic fishes little is known about evolutionary strategies of other Antarctic ectotherms, particularly the abundant benthic incirrate octopods. Their oxygen demand is largely fuelled by the blue oxygen transporter haemocyanin that however, due to its increasing functional failure towards colder temperatures, poses a prime target for cold-adaptive adjustment. While haemocyanin structure has been well understood it remains unclear which molecular features and evolutionary trajectories explain functional properties of octopod haemocyanin. This thesis thus aimed to unravel cold-adaptive features of octopod haemocyanin and the underlying molecular features that evolved to sustain oxygen supply at sub-zero temperatures. Haemocyanin function is best assessed by oxygen binding experiments, which however was challenged due to the minute haemolymph volumes non-model organisms like Antarctic octopods, yield. I thus upgraded an oxygen diffusion chamber with a broad range fibre optic spectrophotometer and a micro-pH optode and tested the setup for Octopus vulgaris, the Antarctic eelpout and a Baikal amphipod. This technical advancement enabled simultaneous recordings of pH and oxygen dependent pigment absorbance in only 15 microliter of sample. Results were highly reproducible and accurate and provided detailed insight to the complex and dynamic spectral features of three diverse blood-types. To identify cold-adaptive functional traits of blood oxygen transport this study compared haemocyanin oxygen binding properties, oxygen carrying capacities and haemolymph protein and ion composition between the Antarctic octopod Pareledone charcoti, the temperate Octopus pallidus and the subtropical Eledone moschata. Compared to octopods from warmer climates, Pareledone charcoti showed incomplete but significantly reduced oxygen ... |
author2 |
Pörtner, Hans Otto Lieb, Bernhard |
format |
Doctoral or Postdoctoral Thesis |
author |
Oellermann, Michael |
author_facet |
Oellermann, Michael |
author_sort |
Oellermann, Michael |
title |
Blue Blood on Ice : Cephalopod haemocyanin function and evolution in a latitudinal cline |
title_short |
Blue Blood on Ice : Cephalopod haemocyanin function and evolution in a latitudinal cline |
title_full |
Blue Blood on Ice : Cephalopod haemocyanin function and evolution in a latitudinal cline |
title_fullStr |
Blue Blood on Ice : Cephalopod haemocyanin function and evolution in a latitudinal cline |
title_full_unstemmed |
Blue Blood on Ice : Cephalopod haemocyanin function and evolution in a latitudinal cline |
title_sort |
blue blood on ice : cephalopod haemocyanin function and evolution in a latitudinal cline |
publisher |
Universität Bremen |
publishDate |
2015 |
url |
https://media.suub.uni-bremen.de/handle/elib/823 https://nbn-resolving.org/urn:nbn:de:gbv:46-00104376-17 |
geographic |
Antarctic Southern Ocean The Antarctic |
geographic_facet |
Antarctic Southern Ocean The Antarctic |
genre |
Antarc* Antarctic Southern Ocean |
genre_facet |
Antarc* Antarctic Southern Ocean |
op_relation |
https://media.suub.uni-bremen.de/handle/elib/823 urn:nbn:de:gbv:46-00104376-17 |
op_rights |
info:eu-repo/semantics/openAccess |
_version_ |
1766275280507240448 |