Photosynthesis in Antarctic sea-ice diatoms
A systematic approach of investigations was applied to obtain precise measurements of photosynthesis under natural conditions in the field from which questions were derived for further analysis in the laboratory. In situ measurements with the tracer 14C through the entire thickness of a young sea ic...
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Universität Bremen
2003
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Online Access: | https://media.suub.uni-bremen.de/handle/elib/1909 https://nbn-resolving.org/urn:nbn:de:gbv:46-diss000005663 |
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ftsubbremen:oai:media.suub.uni-bremen.de:Publications/elib/1909 2023-05-15T13:41:01+02:00 Photosynthesis in Antarctic sea-ice diatoms Photosynthese antarktischer Meereisdiatomeen Mock, Thomas Smetacek, Victor Kirst, Gunter Otto 2003-05-09 application/pdf https://media.suub.uni-bremen.de/handle/elib/1909 https://nbn-resolving.org/urn:nbn:de:gbv:46-diss000005663 eng eng Universität Bremen FB2 Biologie/Chemie https://media.suub.uni-bremen.de/handle/elib/1909 urn:nbn:de:gbv:46-diss000005663 info:eu-repo/semantics/openAccess photosynthesis diatom sea ice Fragilariopsis cylindrus biooptics cold adaptation gene expression EST-library 32 ddc:32 Dissertation doctoralThesis 2003 ftsubbremen 2022-11-09T07:09:41Z A systematic approach of investigations was applied to obtain precise measurements of photosynthesis under natural conditions in the field from which questions were derived for further analysis in the laboratory. In situ measurements with the tracer 14C through the entire thickness of a young sea ice floe revealed that algae are able to actively assimilate dissolved inorganic carbon under extreme conditions after inclusion into newly formed sea ice. These measurements were conducted with ice slices suspended in a new incubator. New sensors (oxygen micro-optodes) were introduced into sea ice research which withstand freezing without damage. A new laboratory sea ice microcosm was developed to simulate natural conditions of sea ice and to cultivating a sea-ice diatom (Fragilariopsis cylindrus) directly within brine channels. However, how changes of environmental conditions influence photosynthesis and how these diatoms are generally adapted to their habitat still remained unresolved. Consequently, the first molecular data base of an obligate psychrophilic diatom (Fragilariopis cylindrus) was conducted using an EST (expressed sequence tag) approach under freezing conditions. More than half (59%) of these sequences could not be identified by GenBank comparison, indicating the existence of many unknown genes. A subset of identified genes and additional genes responsible for photosynthesis, respiration and cold adaptation were cloned and arranged on a macro-array to investigate gene expression under freezing conditions. These molecular measurements were paralleled by biophysical and biochemical investigations. Experimental results revealed that acclimation of diatom photosynthesis under freezing temperatures of sea water and different resource limitations (light, nutrients) requires chaperons and repair mechanisms in order to sustain chloroplast membranes and proteins which are responsible for energy generation and carbon dioxide fixation. Doctoral or Postdoctoral Thesis Antarc* Antarctic Antarktis* Sea ice Media SuUB Bremen (Staats- und Universitätsbibliothek Bremen) Antarctic |
institution |
Open Polar |
collection |
Media SuUB Bremen (Staats- und Universitätsbibliothek Bremen) |
op_collection_id |
ftsubbremen |
language |
English |
topic |
photosynthesis diatom sea ice Fragilariopsis cylindrus biooptics cold adaptation gene expression EST-library 32 ddc:32 |
spellingShingle |
photosynthesis diatom sea ice Fragilariopsis cylindrus biooptics cold adaptation gene expression EST-library 32 ddc:32 Mock, Thomas Photosynthesis in Antarctic sea-ice diatoms |
topic_facet |
photosynthesis diatom sea ice Fragilariopsis cylindrus biooptics cold adaptation gene expression EST-library 32 ddc:32 |
description |
A systematic approach of investigations was applied to obtain precise measurements of photosynthesis under natural conditions in the field from which questions were derived for further analysis in the laboratory. In situ measurements with the tracer 14C through the entire thickness of a young sea ice floe revealed that algae are able to actively assimilate dissolved inorganic carbon under extreme conditions after inclusion into newly formed sea ice. These measurements were conducted with ice slices suspended in a new incubator. New sensors (oxygen micro-optodes) were introduced into sea ice research which withstand freezing without damage. A new laboratory sea ice microcosm was developed to simulate natural conditions of sea ice and to cultivating a sea-ice diatom (Fragilariopsis cylindrus) directly within brine channels. However, how changes of environmental conditions influence photosynthesis and how these diatoms are generally adapted to their habitat still remained unresolved. Consequently, the first molecular data base of an obligate psychrophilic diatom (Fragilariopis cylindrus) was conducted using an EST (expressed sequence tag) approach under freezing conditions. More than half (59%) of these sequences could not be identified by GenBank comparison, indicating the existence of many unknown genes. A subset of identified genes and additional genes responsible for photosynthesis, respiration and cold adaptation were cloned and arranged on a macro-array to investigate gene expression under freezing conditions. These molecular measurements were paralleled by biophysical and biochemical investigations. Experimental results revealed that acclimation of diatom photosynthesis under freezing temperatures of sea water and different resource limitations (light, nutrients) requires chaperons and repair mechanisms in order to sustain chloroplast membranes and proteins which are responsible for energy generation and carbon dioxide fixation. |
author2 |
Smetacek, Victor Kirst, Gunter Otto |
format |
Doctoral or Postdoctoral Thesis |
author |
Mock, Thomas |
author_facet |
Mock, Thomas |
author_sort |
Mock, Thomas |
title |
Photosynthesis in Antarctic sea-ice diatoms |
title_short |
Photosynthesis in Antarctic sea-ice diatoms |
title_full |
Photosynthesis in Antarctic sea-ice diatoms |
title_fullStr |
Photosynthesis in Antarctic sea-ice diatoms |
title_full_unstemmed |
Photosynthesis in Antarctic sea-ice diatoms |
title_sort |
photosynthesis in antarctic sea-ice diatoms |
publisher |
Universität Bremen |
publishDate |
2003 |
url |
https://media.suub.uni-bremen.de/handle/elib/1909 https://nbn-resolving.org/urn:nbn:de:gbv:46-diss000005663 |
geographic |
Antarctic |
geographic_facet |
Antarctic |
genre |
Antarc* Antarctic Antarktis* Sea ice |
genre_facet |
Antarc* Antarctic Antarktis* Sea ice |
op_relation |
https://media.suub.uni-bremen.de/handle/elib/1909 urn:nbn:de:gbv:46-diss000005663 |
op_rights |
info:eu-repo/semantics/openAccess |
_version_ |
1766144593019011072 |