Freshwater Chlorobia Exhibit Metabolic Specialization among Cosmopolitan and Endemic Populations
Photosynthetic bacteria from the class Chlorobia (formerly phylum Chlorobi) sustain carbon fixation in anoxic water columns. They harvest light at extremely low intensities and use various inorganic electron donors to fix carbon dioxide into biomass. Until now, most information on the functional eco...
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Stockholms universitet, Institutionen för ekologi, miljö och botanik
2021
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ftstockholmuniv:oai:DiVA.org:su-193247 2023-05-15T18:28:30+02:00 Freshwater Chlorobia Exhibit Metabolic Specialization among Cosmopolitan and Endemic Populations Garcia, Sarahi L. Mehrshad, Maliheh Buck, Moritz Tsuji, Jackson Neufeld, Josh McMahon, Katherine Bertilsson, Stefan Greening, Chris Peura, Sari 2021 application/pdf http://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-193247 https://doi.org/10.1128/mSystems.01196-20 eng eng Stockholms universitet, Institutionen för ekologi, miljö och botanik Uppsala University, Sweden mSystems, 2021, 6:3, orcid:0000-0002-8622-0308 http://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-193247 doi:10.1128/mSystems.01196-20 ISI:000709811800011 info:eu-repo/semantics/openAccess Chlorobia freshwater photosynthetic bacteria planktonic Ecology Ekologi Article in journal info:eu-repo/semantics/article text 2021 ftstockholmuniv https://doi.org/10.1128/mSystems.01196-20 2023-02-23T21:42:36Z Photosynthetic bacteria from the class Chlorobia (formerly phylum Chlorobi) sustain carbon fixation in anoxic water columns. They harvest light at extremely low intensities and use various inorganic electron donors to fix carbon dioxide into biomass. Until now, most information on the functional ecology and local adaptations of Chlorobia members came from isolates and merely 26 sequenced genomes that may not adequately represent natural populations. To address these limitations, we analyzed global metagenomes to profile planktonic Chlorobia cells from the oxyclines of 42 freshwater bodies, spanning subarctic to tropical regions and encompassing all four seasons. We assembled and compiled over 500 genomes, including metagenome-assembled genomes (MAGs), single-amplified genomes (SAGs), and reference genomes from cultures, clustering them into 71 metagenomic operational taxonomic units (mOTUs or “species”). Of the 71 mOTUs, 57 were classified within the genus Chlorobium, and these mOTUs represented up to ∼60% of the microbial communities in the sampled anoxic waters. Several Chlorobium-associated mOTUs were globally distributed, whereas others were endemic to individual lakes. Although most clades encoded the ability to oxidize hydrogen, many lacked genes for the oxidation of specific sulfur and iron substrates. Surprisingly, one globally distributed Scandinavian clade encoded the ability to oxidize hydrogen, sulfur, and iron, suggesting that metabolic versatility facilitated such widespread colonization. Overall, these findings provide new insight into the biogeography of the Chlorobia and the metabolic traits that facilitate niche specialization within lake ecosystems. Article in Journal/Newspaper Subarctic Stockholm University: Publications (DiVA) mSystems 6 3 |
institution |
Open Polar |
collection |
Stockholm University: Publications (DiVA) |
op_collection_id |
ftstockholmuniv |
language |
English |
topic |
Chlorobia freshwater photosynthetic bacteria planktonic Ecology Ekologi |
spellingShingle |
Chlorobia freshwater photosynthetic bacteria planktonic Ecology Ekologi Garcia, Sarahi L. Mehrshad, Maliheh Buck, Moritz Tsuji, Jackson Neufeld, Josh McMahon, Katherine Bertilsson, Stefan Greening, Chris Peura, Sari Freshwater Chlorobia Exhibit Metabolic Specialization among Cosmopolitan and Endemic Populations |
topic_facet |
Chlorobia freshwater photosynthetic bacteria planktonic Ecology Ekologi |
description |
Photosynthetic bacteria from the class Chlorobia (formerly phylum Chlorobi) sustain carbon fixation in anoxic water columns. They harvest light at extremely low intensities and use various inorganic electron donors to fix carbon dioxide into biomass. Until now, most information on the functional ecology and local adaptations of Chlorobia members came from isolates and merely 26 sequenced genomes that may not adequately represent natural populations. To address these limitations, we analyzed global metagenomes to profile planktonic Chlorobia cells from the oxyclines of 42 freshwater bodies, spanning subarctic to tropical regions and encompassing all four seasons. We assembled and compiled over 500 genomes, including metagenome-assembled genomes (MAGs), single-amplified genomes (SAGs), and reference genomes from cultures, clustering them into 71 metagenomic operational taxonomic units (mOTUs or “species”). Of the 71 mOTUs, 57 were classified within the genus Chlorobium, and these mOTUs represented up to ∼60% of the microbial communities in the sampled anoxic waters. Several Chlorobium-associated mOTUs were globally distributed, whereas others were endemic to individual lakes. Although most clades encoded the ability to oxidize hydrogen, many lacked genes for the oxidation of specific sulfur and iron substrates. Surprisingly, one globally distributed Scandinavian clade encoded the ability to oxidize hydrogen, sulfur, and iron, suggesting that metabolic versatility facilitated such widespread colonization. Overall, these findings provide new insight into the biogeography of the Chlorobia and the metabolic traits that facilitate niche specialization within lake ecosystems. |
format |
Article in Journal/Newspaper |
author |
Garcia, Sarahi L. Mehrshad, Maliheh Buck, Moritz Tsuji, Jackson Neufeld, Josh McMahon, Katherine Bertilsson, Stefan Greening, Chris Peura, Sari |
author_facet |
Garcia, Sarahi L. Mehrshad, Maliheh Buck, Moritz Tsuji, Jackson Neufeld, Josh McMahon, Katherine Bertilsson, Stefan Greening, Chris Peura, Sari |
author_sort |
Garcia, Sarahi L. |
title |
Freshwater Chlorobia Exhibit Metabolic Specialization among Cosmopolitan and Endemic Populations |
title_short |
Freshwater Chlorobia Exhibit Metabolic Specialization among Cosmopolitan and Endemic Populations |
title_full |
Freshwater Chlorobia Exhibit Metabolic Specialization among Cosmopolitan and Endemic Populations |
title_fullStr |
Freshwater Chlorobia Exhibit Metabolic Specialization among Cosmopolitan and Endemic Populations |
title_full_unstemmed |
Freshwater Chlorobia Exhibit Metabolic Specialization among Cosmopolitan and Endemic Populations |
title_sort |
freshwater chlorobia exhibit metabolic specialization among cosmopolitan and endemic populations |
publisher |
Stockholms universitet, Institutionen för ekologi, miljö och botanik |
publishDate |
2021 |
url |
http://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-193247 https://doi.org/10.1128/mSystems.01196-20 |
genre |
Subarctic |
genre_facet |
Subarctic |
op_relation |
mSystems, 2021, 6:3, orcid:0000-0002-8622-0308 http://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-193247 doi:10.1128/mSystems.01196-20 ISI:000709811800011 |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1128/mSystems.01196-20 |
container_title |
mSystems |
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6 |
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3 |
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1766211015873134592 |