Myo-inositol phosphate synthase expression in the European eel (Anguilla anguilla) and Nile tilapia (Oreochromis niloticus) : effect of seawater acclimation
The work was funded by a research grant awarded to GC and NH by the Natural Environment Research Council (NE/J010081/1). A single MIPS gene (Isyna1/Ino1) exists in eel and tilapia genomes with a single MIPS transcript identified in all eel tissues, although two MIPS spliced variants (termed MIPS(s)...
Published in: | American Journal of Physiology-Regulatory, Integrative and Comparative Physiology |
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ftstandrewserep:oai:research-repository.st-andrews.ac.uk:10023/9278 2023-07-02T03:29:36+02:00 Myo-inositol phosphate synthase expression in the European eel (Anguilla anguilla) and Nile tilapia (Oreochromis niloticus) : effect of seawater acclimation Kalujnaia, Svetlana Hazon, Neil Cramb, Gordon NERC BBSRC University of St Andrews. School of Medicine University of St Andrews. School of Biology University of St Andrews. Marine Alliance for Science & Technology Scotland University of St Andrews. Scottish Oceans Institute 2016-08-09T12:32:34Z application/pdf http://hdl.handle.net/10023/9278 https://doi.org/10.1152/ajpregu.00056.2016 eng eng American Journal of Physiology. Regulatory, Integrative and Comparative Physiology Kalujnaia , S , Hazon , N & Cramb , G 2016 , ' Myo-inositol phosphate synthase expression in the European eel ( Anguilla anguilla ) and Nile tilapia ( Oreochromis niloticus ) : effect of seawater acclimation ' , American Journal of Physiology. Regulatory, Integrative and Comparative Physiology , vol. 311 , no. 2 , pp. R287-R298 . https://doi.org/10.1152/ajpregu.00056.2016 0363-6119 PURE: 242974751 PURE UUID: d01a39d6-fcf5-4a97-86d5-294dc7f1eaa9 Scopus: 84984655405 WOS: 000384770100010 ORCID: /0000-0003-4929-951X/work/64033638 http://hdl.handle.net/10023/9278 https://doi.org/10.1152/ajpregu.00056.2016 NE/J010081/1 BB/M026019/1 Copyright © 2016 the American Physiological Society Licensed under Creative Commons Attribution CC-BY 3.0: © the American Physiological Society Anguilla anguilla Oreochromis niloticus Teleost Myoinositol phosphotase synthase Myo-D-inositol 3-phosphatase synthase Osmoregulation QH426 Genetics QP Physiology R Medicine NDAS QH426 QP R Journal article 2016 ftstandrewserep https://doi.org/10.1152/ajpregu.00056.2016 2023-06-13T18:26:34Z The work was funded by a research grant awarded to GC and NH by the Natural Environment Research Council (NE/J010081/1). A single MIPS gene (Isyna1/Ino1) exists in eel and tilapia genomes with a single MIPS transcript identified in all eel tissues, although two MIPS spliced variants (termed MIPS(s) and MIPS(l)) are found in all tilapia tissues. The larger tilapia transcript (MIPS(l)) results from the inclusion of the 87-nucleotide intron between exons 5 and 6 in the genomic sequence. In most tilapia tissues the MIPS(s) transcript exhibits much higher abundance (generally >10-fold) with the exception of white skeletal muscle and oocytes where the MIPS(l) transcript predominates. SW-acclimation resulted in large (6- to 32-fold) increases in mRNA expression for both MIPS(s) and MIPS(l) in all tilapia tissues tested whereas in the eel changes in expression were limited to a more modest 2.5-fold increase and only in the kidney. Western blots identified a number of species and tissue-specific immunoreactive MIPS proteins ranging from 40 to 67 kDa molecular weight. SW-acclimation failed to affect the abundance of any immunoreactive protein in any tissue tested from the eel. However, a major 67 kDa immunoreactive protein (presumed to be MIPS) found in tilapia tissues exhibited 11- and 54-fold increases in expression in gill and fin samples from SW-acclimated fish. Immunohistochemical investigations revealed specific immunoreactivity in the gill, fin, skin and intestine taken from only SW acclimated tilapia. Immunofluorescence indicated that MIPS was expressed within gill chondrocytes and epithelial cells of the primary filaments, basal epithelial cell layers of the skin and fin, the cytosol of columnar intestinal epithelial and mucous cells as well as unknown entero-endocrine-like cells. Publisher PDF Peer reviewed Article in Journal/Newspaper Anguilla anguilla European eel University of St Andrews: Digital Research Repository American Journal of Physiology-Regulatory, Integrative and Comparative Physiology 311 2 R287 R298 |
institution |
Open Polar |
collection |
University of St Andrews: Digital Research Repository |
op_collection_id |
ftstandrewserep |
language |
English |
topic |
Anguilla anguilla Oreochromis niloticus Teleost Myoinositol phosphotase synthase Myo-D-inositol 3-phosphatase synthase Osmoregulation QH426 Genetics QP Physiology R Medicine NDAS QH426 QP R |
spellingShingle |
Anguilla anguilla Oreochromis niloticus Teleost Myoinositol phosphotase synthase Myo-D-inositol 3-phosphatase synthase Osmoregulation QH426 Genetics QP Physiology R Medicine NDAS QH426 QP R Kalujnaia, Svetlana Hazon, Neil Cramb, Gordon Myo-inositol phosphate synthase expression in the European eel (Anguilla anguilla) and Nile tilapia (Oreochromis niloticus) : effect of seawater acclimation |
topic_facet |
Anguilla anguilla Oreochromis niloticus Teleost Myoinositol phosphotase synthase Myo-D-inositol 3-phosphatase synthase Osmoregulation QH426 Genetics QP Physiology R Medicine NDAS QH426 QP R |
description |
The work was funded by a research grant awarded to GC and NH by the Natural Environment Research Council (NE/J010081/1). A single MIPS gene (Isyna1/Ino1) exists in eel and tilapia genomes with a single MIPS transcript identified in all eel tissues, although two MIPS spliced variants (termed MIPS(s) and MIPS(l)) are found in all tilapia tissues. The larger tilapia transcript (MIPS(l)) results from the inclusion of the 87-nucleotide intron between exons 5 and 6 in the genomic sequence. In most tilapia tissues the MIPS(s) transcript exhibits much higher abundance (generally >10-fold) with the exception of white skeletal muscle and oocytes where the MIPS(l) transcript predominates. SW-acclimation resulted in large (6- to 32-fold) increases in mRNA expression for both MIPS(s) and MIPS(l) in all tilapia tissues tested whereas in the eel changes in expression were limited to a more modest 2.5-fold increase and only in the kidney. Western blots identified a number of species and tissue-specific immunoreactive MIPS proteins ranging from 40 to 67 kDa molecular weight. SW-acclimation failed to affect the abundance of any immunoreactive protein in any tissue tested from the eel. However, a major 67 kDa immunoreactive protein (presumed to be MIPS) found in tilapia tissues exhibited 11- and 54-fold increases in expression in gill and fin samples from SW-acclimated fish. Immunohistochemical investigations revealed specific immunoreactivity in the gill, fin, skin and intestine taken from only SW acclimated tilapia. Immunofluorescence indicated that MIPS was expressed within gill chondrocytes and epithelial cells of the primary filaments, basal epithelial cell layers of the skin and fin, the cytosol of columnar intestinal epithelial and mucous cells as well as unknown entero-endocrine-like cells. Publisher PDF Peer reviewed |
author2 |
NERC BBSRC University of St Andrews. School of Medicine University of St Andrews. School of Biology University of St Andrews. Marine Alliance for Science & Technology Scotland University of St Andrews. Scottish Oceans Institute |
format |
Article in Journal/Newspaper |
author |
Kalujnaia, Svetlana Hazon, Neil Cramb, Gordon |
author_facet |
Kalujnaia, Svetlana Hazon, Neil Cramb, Gordon |
author_sort |
Kalujnaia, Svetlana |
title |
Myo-inositol phosphate synthase expression in the European eel (Anguilla anguilla) and Nile tilapia (Oreochromis niloticus) : effect of seawater acclimation |
title_short |
Myo-inositol phosphate synthase expression in the European eel (Anguilla anguilla) and Nile tilapia (Oreochromis niloticus) : effect of seawater acclimation |
title_full |
Myo-inositol phosphate synthase expression in the European eel (Anguilla anguilla) and Nile tilapia (Oreochromis niloticus) : effect of seawater acclimation |
title_fullStr |
Myo-inositol phosphate synthase expression in the European eel (Anguilla anguilla) and Nile tilapia (Oreochromis niloticus) : effect of seawater acclimation |
title_full_unstemmed |
Myo-inositol phosphate synthase expression in the European eel (Anguilla anguilla) and Nile tilapia (Oreochromis niloticus) : effect of seawater acclimation |
title_sort |
myo-inositol phosphate synthase expression in the european eel (anguilla anguilla) and nile tilapia (oreochromis niloticus) : effect of seawater acclimation |
publishDate |
2016 |
url |
http://hdl.handle.net/10023/9278 https://doi.org/10.1152/ajpregu.00056.2016 |
genre |
Anguilla anguilla European eel |
genre_facet |
Anguilla anguilla European eel |
op_relation |
American Journal of Physiology. Regulatory, Integrative and Comparative Physiology Kalujnaia , S , Hazon , N & Cramb , G 2016 , ' Myo-inositol phosphate synthase expression in the European eel ( Anguilla anguilla ) and Nile tilapia ( Oreochromis niloticus ) : effect of seawater acclimation ' , American Journal of Physiology. Regulatory, Integrative and Comparative Physiology , vol. 311 , no. 2 , pp. R287-R298 . https://doi.org/10.1152/ajpregu.00056.2016 0363-6119 PURE: 242974751 PURE UUID: d01a39d6-fcf5-4a97-86d5-294dc7f1eaa9 Scopus: 84984655405 WOS: 000384770100010 ORCID: /0000-0003-4929-951X/work/64033638 http://hdl.handle.net/10023/9278 https://doi.org/10.1152/ajpregu.00056.2016 NE/J010081/1 BB/M026019/1 |
op_rights |
Copyright © 2016 the American Physiological Society Licensed under Creative Commons Attribution CC-BY 3.0: © the American Physiological Society |
op_doi |
https://doi.org/10.1152/ajpregu.00056.2016 |
container_title |
American Journal of Physiology-Regulatory, Integrative and Comparative Physiology |
container_volume |
311 |
container_issue |
2 |
container_start_page |
R287 |
op_container_end_page |
R298 |
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1770270740900216832 |