An alternative method for correcting fluorescence quenching
This work received funding from the MASTS pooling initiative (The Marine Alliance for Science and Technology for Scotland) and their support is gratefully acknowledged. MASTS is funded by the Scottish Funding Council (grant reference HR09011) and contributing institutions. Under high light intensity...
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ftstandrewserep:oai:research-repository.st-andrews.ac.uk:10023/5042 2023-07-02T03:32:08+02:00 An alternative method for correcting fluorescence quenching Biermann, Lauren Guinet, Christophe Bester, Marthan Brierley, Andrew Stuart Boehme, Lars NERC University of St Andrews. School of Biology University of St Andrews. Marine Alliance for Science & Technology Scotland University of St Andrews. Sea Mammal Research Unit University of St Andrews. Scottish Oceans Institute University of St Andrews. Centre for Research into Ecological & Environmental Modelling University of St Andrews. Pelagic Ecology Research Group 2014-07-21T11:01:04Z 9 application/pdf http://hdl.handle.net/10023/5042 https://doi.org/10.5194/os-11-83-2015 eng eng Ocean Science Biermann , L , Guinet , C , Bester , M , Brierley , A S & Boehme , L 2015 , ' An alternative method for correcting fluorescence quenching ' , Ocean Science , vol. 11 , no. 1 , pp. 83-91 . https://doi.org/10.5194/os-11-83-2015 1812-0784 PURE: 126140108 PURE UUID: 91d48292-2076-4960-bcfe-7d14ea94c76e Scopus: 84920995429 ORCID: /0000-0002-6438-6892/work/60427315 WOS: 000350556600006 http://hdl.handle.net/10023/5042 https://doi.org/10.5194/os-11-83-2015 NE/E018289/1 NE/G014833/1 © Author(s) 2015. This work is distributed under the Creative Commons Attribution 3.0 License QK Botany GC Oceanography NDAS QK GC Journal article 2014 ftstandrewserep https://doi.org/10.5194/os-11-83-2015 2023-06-13T18:29:02Z This work received funding from the MASTS pooling initiative (The Marine Alliance for Science and Technology for Scotland) and their support is gratefully acknowledged. MASTS is funded by the Scottish Funding Council (grant reference HR09011) and contributing institutions. Under high light intensity, phytoplankton protect their photosystems from bleaching through non-photochemical quenching processes. The consequence of this is suppression of fluorescence emission, which must be corrected when measuring in situ yield with fluorometers. We present data from the Southern Ocean, collected over five austral summers by 19 southern elephant seals tagged with fluorometers. Conventionally, fluorescence data collected during the day (quenched) were corrected using the limit of the mixed layer, assuming that phytoplankton are uniformly mixed from the surface to this depth. However, distinct deep fluorescence maxima were measured in approximately 30% of the night (unquenched) data. To account for the evidence that chlorophyll is not uniformly mixed in the upper layer, we propose correcting from the limit of the euphotic zone, defined as the depth at which photosynthetically available radiation is ~ 1% of the surface value. Mixed layer depth exceeded euphotic depth over 80% of the time. Under these conditions, quenching was corrected from the depth of the remotely derived euphotic zone Zeu, and compared with fluorescence corrected from the depth of the density-derived mixed layer. Deep fluorescence maxima were evident in only 10% of the day data when correcting from mixed layer depth. This was doubled to 21% when correcting from Zeu, more closely matching the unquenched (night) data. Furthermore, correcting from Zeu served to conserve non-uniform chlorophyll features found between the 1% light level and mixed layer depth. Publisher PDF Publisher PDF Peer reviewed Article in Journal/Newspaper Elephant Seals Southern Elephant Seals Southern Ocean University of St Andrews: Digital Research Repository Austral Southern Ocean Ocean Science 11 1 83 91 |
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Open Polar |
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University of St Andrews: Digital Research Repository |
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ftstandrewserep |
language |
English |
topic |
QK Botany GC Oceanography NDAS QK GC |
spellingShingle |
QK Botany GC Oceanography NDAS QK GC Biermann, Lauren Guinet, Christophe Bester, Marthan Brierley, Andrew Stuart Boehme, Lars An alternative method for correcting fluorescence quenching |
topic_facet |
QK Botany GC Oceanography NDAS QK GC |
description |
This work received funding from the MASTS pooling initiative (The Marine Alliance for Science and Technology for Scotland) and their support is gratefully acknowledged. MASTS is funded by the Scottish Funding Council (grant reference HR09011) and contributing institutions. Under high light intensity, phytoplankton protect their photosystems from bleaching through non-photochemical quenching processes. The consequence of this is suppression of fluorescence emission, which must be corrected when measuring in situ yield with fluorometers. We present data from the Southern Ocean, collected over five austral summers by 19 southern elephant seals tagged with fluorometers. Conventionally, fluorescence data collected during the day (quenched) were corrected using the limit of the mixed layer, assuming that phytoplankton are uniformly mixed from the surface to this depth. However, distinct deep fluorescence maxima were measured in approximately 30% of the night (unquenched) data. To account for the evidence that chlorophyll is not uniformly mixed in the upper layer, we propose correcting from the limit of the euphotic zone, defined as the depth at which photosynthetically available radiation is ~ 1% of the surface value. Mixed layer depth exceeded euphotic depth over 80% of the time. Under these conditions, quenching was corrected from the depth of the remotely derived euphotic zone Zeu, and compared with fluorescence corrected from the depth of the density-derived mixed layer. Deep fluorescence maxima were evident in only 10% of the day data when correcting from mixed layer depth. This was doubled to 21% when correcting from Zeu, more closely matching the unquenched (night) data. Furthermore, correcting from Zeu served to conserve non-uniform chlorophyll features found between the 1% light level and mixed layer depth. Publisher PDF Publisher PDF Peer reviewed |
author2 |
NERC University of St Andrews. School of Biology University of St Andrews. Marine Alliance for Science & Technology Scotland University of St Andrews. Sea Mammal Research Unit University of St Andrews. Scottish Oceans Institute University of St Andrews. Centre for Research into Ecological & Environmental Modelling University of St Andrews. Pelagic Ecology Research Group |
format |
Article in Journal/Newspaper |
author |
Biermann, Lauren Guinet, Christophe Bester, Marthan Brierley, Andrew Stuart Boehme, Lars |
author_facet |
Biermann, Lauren Guinet, Christophe Bester, Marthan Brierley, Andrew Stuart Boehme, Lars |
author_sort |
Biermann, Lauren |
title |
An alternative method for correcting fluorescence quenching |
title_short |
An alternative method for correcting fluorescence quenching |
title_full |
An alternative method for correcting fluorescence quenching |
title_fullStr |
An alternative method for correcting fluorescence quenching |
title_full_unstemmed |
An alternative method for correcting fluorescence quenching |
title_sort |
alternative method for correcting fluorescence quenching |
publishDate |
2014 |
url |
http://hdl.handle.net/10023/5042 https://doi.org/10.5194/os-11-83-2015 |
geographic |
Austral Southern Ocean |
geographic_facet |
Austral Southern Ocean |
genre |
Elephant Seals Southern Elephant Seals Southern Ocean |
genre_facet |
Elephant Seals Southern Elephant Seals Southern Ocean |
op_relation |
Ocean Science Biermann , L , Guinet , C , Bester , M , Brierley , A S & Boehme , L 2015 , ' An alternative method for correcting fluorescence quenching ' , Ocean Science , vol. 11 , no. 1 , pp. 83-91 . https://doi.org/10.5194/os-11-83-2015 1812-0784 PURE: 126140108 PURE UUID: 91d48292-2076-4960-bcfe-7d14ea94c76e Scopus: 84920995429 ORCID: /0000-0002-6438-6892/work/60427315 WOS: 000350556600006 http://hdl.handle.net/10023/5042 https://doi.org/10.5194/os-11-83-2015 NE/E018289/1 NE/G014833/1 |
op_rights |
© Author(s) 2015. This work is distributed under the Creative Commons Attribution 3.0 License |
op_doi |
https://doi.org/10.5194/os-11-83-2015 |
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Ocean Science |
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11 |
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1 |
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83 |
op_container_end_page |
91 |
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1770271640007999488 |