Phenotypic and evolutionary variation in fish myofibrillar proteins

Chapter 1 General Introduction: The general introduction initially presents the major landmarks in muscle research of the last 3 millennia. The proteins of the contractile apparatus and their role in muscle contraction are described. There is then a description of how contractile proteins are known...

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Bibliographic Details
Main Author: Cole, Nicholas James
Other Authors: Johnston, Ian A.
Format: Doctoral or Postdoctoral Thesis
Language:English
Published: University of St Andrews 2018
Subjects:
Online Access:http://hdl.handle.net/10023/15050
Description
Summary:Chapter 1 General Introduction: The general introduction initially presents the major landmarks in muscle research of the last 3 millennia. The proteins of the contractile apparatus and their role in muscle contraction are described. There is then a description of how contractile proteins are known to alter through the expression of isoforms. Finally, a description of fish muscle and its fibre types is given, followed by the aims of the thesis. Chapter 2 Materials and Methods: An account is given of the materials and methods used throughout this thesis. This includes myofibril preparation and electrophoretic techniques. The techniques described are one and two dimensional polyacrylamide gel electrophoresis (PAGE), iso-electric focusing (lEF), peptide mapping, and one and two dimensional alkali-urea PAGE. The methods used to fix, stain and store the gels are then given, followed by the protocol used for Western blotting. Finally, the analysis of proteins bands is described. Chapter 3 Temperature and the plasticity of myofibrillar proteins during ontogeny in the Atlantic herring (Clupea harengus L.): Many aspects of development are influenced by temperature. The aim of this study was to investigate the effect of rearing temperature on the development and myofibrillar protein expression during ontogeny in the Atlantic herring (Clupea harengus L.). Chapter 4 The effect of body size on the myofibrillar protein composition of fast muscle fibres in the short horn sculpin (Myoxocephalus scorpius L.): The contractile properties of muscle vary with body length in fish. The aim of this study was determine if the proteins altered with body size in the short-horn sculpin (Myoxocephalus scorpius L.). Furthermore, could changes in protein expression be related to differences in contractile properties. Chapter 5 The myofibrillar proteins of Antarctic and sub-Antarctic fish: The myosins of Antarctic fish are specialised for function at low temperature. The aim of this study was to determine if the myofibrillar proteins present ...