Not just fat: investigating the proteome of cetacean blubber tissue
Mammalian adipose tissue is increasingly being recognized as an endocrine organ involved in the regulation of a number of metabolic processes and pathways. It responds to signals from different hormone systems and the central nervous system, and expresses a variety of protein factors with important...
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2018
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Online Access: | https://pure.sruc.ac.uk/en/publications/7639bd0f-324e-421e-b3d7-4f10bca378fc https://doi.org/10.1093/conphys/coy003 https://pure.sruc.ac.uk/ws/files/15264671/14934.pdf |
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ftsrucpubl:oai:pure.atira.dk:publications/7639bd0f-324e-421e-b3d7-4f10bca378fc 2024-06-09T07:49:03+00:00 Not just fat: investigating the proteome of cetacean blubber tissue Kershaw, JL Botting, CH Brownlow, A Hall, AJ 2018-02-16 application/pdf https://pure.sruc.ac.uk/en/publications/7639bd0f-324e-421e-b3d7-4f10bca378fc https://doi.org/10.1093/conphys/coy003 https://pure.sruc.ac.uk/ws/files/15264671/14934.pdf eng eng https://pure.sruc.ac.uk/en/publications/7639bd0f-324e-421e-b3d7-4f10bca378fc info:eu-repo/semantics/openAccess Kershaw , JL , Botting , CH , Brownlow , A & Hall , AJ 2018 , ' Not just fat: investigating the proteome of cetacean blubber tissue ' , Conservation Physiology , vol. 6 , no. 1 , coy003 . https://doi.org/10.1093/conphys/coy003 Adipose tissue Biomarkers Marine mammals Metabolism Proteomics /dk/atira/pure/sustainabledevelopmentgoals/life_below_water name=SDG 14 - Life Below Water article 2018 ftsrucpubl https://doi.org/10.1093/conphys/coy003 2024-05-16T14:28:29Z Mammalian adipose tissue is increasingly being recognized as an endocrine organ involved in the regulation of a number of metabolic processes and pathways. It responds to signals from different hormone systems and the central nervous system, and expresses a variety of protein factors with important paracrine and endocrine functions. This study presents a first step towards the systematic analysis of the protein content of cetacean adipose tissue, the blubber, in order to investigate the kinds of proteins present and their relative abundance. Full depth blubber subsamples were collected from dead-stranded harbour porpoises (Phocoena phocoena) (n = 21). Three total protein extraction methods were trialled, and the highest total protein yields with the lowest extraction variability were achieved using a RIPA cell lysis and extraction buffer based protocol. Extracted proteins were separated using 1D Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis (SDS-PAGE), and identified using nanoflow Liquid Chromatography Electrospray Ionization in tandem with Mass Spectrometry (nLC-ESI–MS/MS). A range of proteins were identified (n = 295) and classed into eight functional groups, the most abundant of which were involved in cell function and metabolism (45%), immune response and inflammation (15%) and lipid metabolism (11%). These proteins likely originate both from the various cell types within the blubber tissue itself, and from the circulation. They therefore have the potential to capture information on the cellular and physiological stresses experienced by individuals at the time of sampling. The importance of this proteomic approach is two-fold: Firstly, it could help to assign novel functions to marine mammal blubber in keeping with current understanding of the multi-functional role of adipose tissue in other mammals. Secondly, it could lead to the development of a suite of biomarkers to better monitor the physiological state and health of live individuals though remote blubber biopsy sampling. Article in Journal/Newspaper Phocoena phocoena SRUC (Scotland's Rural College): Research Portal Conservation Physiology 6 1 |
institution |
Open Polar |
collection |
SRUC (Scotland's Rural College): Research Portal |
op_collection_id |
ftsrucpubl |
language |
English |
topic |
Adipose tissue Biomarkers Marine mammals Metabolism Proteomics /dk/atira/pure/sustainabledevelopmentgoals/life_below_water name=SDG 14 - Life Below Water |
spellingShingle |
Adipose tissue Biomarkers Marine mammals Metabolism Proteomics /dk/atira/pure/sustainabledevelopmentgoals/life_below_water name=SDG 14 - Life Below Water Kershaw, JL Botting, CH Brownlow, A Hall, AJ Not just fat: investigating the proteome of cetacean blubber tissue |
topic_facet |
Adipose tissue Biomarkers Marine mammals Metabolism Proteomics /dk/atira/pure/sustainabledevelopmentgoals/life_below_water name=SDG 14 - Life Below Water |
description |
Mammalian adipose tissue is increasingly being recognized as an endocrine organ involved in the regulation of a number of metabolic processes and pathways. It responds to signals from different hormone systems and the central nervous system, and expresses a variety of protein factors with important paracrine and endocrine functions. This study presents a first step towards the systematic analysis of the protein content of cetacean adipose tissue, the blubber, in order to investigate the kinds of proteins present and their relative abundance. Full depth blubber subsamples were collected from dead-stranded harbour porpoises (Phocoena phocoena) (n = 21). Three total protein extraction methods were trialled, and the highest total protein yields with the lowest extraction variability were achieved using a RIPA cell lysis and extraction buffer based protocol. Extracted proteins were separated using 1D Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis (SDS-PAGE), and identified using nanoflow Liquid Chromatography Electrospray Ionization in tandem with Mass Spectrometry (nLC-ESI–MS/MS). A range of proteins were identified (n = 295) and classed into eight functional groups, the most abundant of which were involved in cell function and metabolism (45%), immune response and inflammation (15%) and lipid metabolism (11%). These proteins likely originate both from the various cell types within the blubber tissue itself, and from the circulation. They therefore have the potential to capture information on the cellular and physiological stresses experienced by individuals at the time of sampling. The importance of this proteomic approach is two-fold: Firstly, it could help to assign novel functions to marine mammal blubber in keeping with current understanding of the multi-functional role of adipose tissue in other mammals. Secondly, it could lead to the development of a suite of biomarkers to better monitor the physiological state and health of live individuals though remote blubber biopsy sampling. |
format |
Article in Journal/Newspaper |
author |
Kershaw, JL Botting, CH Brownlow, A Hall, AJ |
author_facet |
Kershaw, JL Botting, CH Brownlow, A Hall, AJ |
author_sort |
Kershaw, JL |
title |
Not just fat: investigating the proteome of cetacean blubber tissue |
title_short |
Not just fat: investigating the proteome of cetacean blubber tissue |
title_full |
Not just fat: investigating the proteome of cetacean blubber tissue |
title_fullStr |
Not just fat: investigating the proteome of cetacean blubber tissue |
title_full_unstemmed |
Not just fat: investigating the proteome of cetacean blubber tissue |
title_sort |
not just fat: investigating the proteome of cetacean blubber tissue |
publishDate |
2018 |
url |
https://pure.sruc.ac.uk/en/publications/7639bd0f-324e-421e-b3d7-4f10bca378fc https://doi.org/10.1093/conphys/coy003 https://pure.sruc.ac.uk/ws/files/15264671/14934.pdf |
genre |
Phocoena phocoena |
genre_facet |
Phocoena phocoena |
op_source |
Kershaw , JL , Botting , CH , Brownlow , A & Hall , AJ 2018 , ' Not just fat: investigating the proteome of cetacean blubber tissue ' , Conservation Physiology , vol. 6 , no. 1 , coy003 . https://doi.org/10.1093/conphys/coy003 |
op_relation |
https://pure.sruc.ac.uk/en/publications/7639bd0f-324e-421e-b3d7-4f10bca378fc |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1093/conphys/coy003 |
container_title |
Conservation Physiology |
container_volume |
6 |
container_issue |
1 |
_version_ |
1801381145899696128 |