Spawning-dependent stress responses in Pacific oysters, Crassostrea gigas: a simulated bacterial challenge in oysters
This study investigated the effect of spawning activity on the ability of oysters to respond to a simulated bacterial challenge, as a possible explanation for summer mortality. Using injection with a nonviable bacterial solution (NBS) of Vibrio harveyi, comparisons of immune parameters and biochemic...
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Online Access: | https://epubs.scu.edu.au/esm_pubs/3581 https://doi.org/10.1016/j.aquaculture.2009.04.044 |
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ftsoutherncu:oai:epubs.scu.edu.au:esm_pubs-4609 2023-05-15T15:57:58+02:00 Spawning-dependent stress responses in Pacific oysters, Crassostrea gigas: a simulated bacterial challenge in oysters Li, Yan Qin, Jian G Li, Xiaoxu Benkendorff, Kirsten, Dr 2009-01-01T08:00:00Z https://epubs.scu.edu.au/esm_pubs/3581 https://doi.org/10.1016/j.aquaculture.2009.04.044 unknown ePublications@SCU School of Environment, Science and Engineering Papers Oyster mortality simulated bacterial challenge spawning stress response immunosuppression energy reserve adenylate energy charge Environmental Sciences article 2009 ftsoutherncu https://doi.org/10.1016/j.aquaculture.2009.04.044 2019-08-06T13:18:04Z This study investigated the effect of spawning activity on the ability of oysters to respond to a simulated bacterial challenge, as a possible explanation for summer mortality. Using injection with a nonviable bacterial solution (NBS) of Vibrio harveyi, comparisons of immune parameters and biochemical stress responses were made between pre- and post-spawning Crassostrea gigas. Oyster mortality was also examined with extracellular products injection from V. harveyi cultures, with post-spawning oysters suffering significantly greater mortality than pre-spawning oysters. Oyster cellular immune defence to NBS was not influenced by spawning, indicated by comparable phagocytosis rates in pre- and post-spawning oysters. However, hemolymph antimicrobial activity was significantly diminished in post-spawning oysters, coincident with a reduction of hemolymph protein. The analysis of glycogen reserves demonstrated that the recovery process of post-spawning oysters was impaired by NBS challenge, resulting in a suppressed metabolic activity, indicated by lower adenylate energy charge. These results suggest that the energy cost of spawning compromises the immune and metabolic responses of oysters, leaving them more vulnerable to pathogenic challenge. Article in Journal/Newspaper Crassostrea gigas Southern Cross University: epublications@SCU Pacific Aquaculture 293 3-4 164 171 |
institution |
Open Polar |
collection |
Southern Cross University: epublications@SCU |
op_collection_id |
ftsoutherncu |
language |
unknown |
topic |
Oyster mortality simulated bacterial challenge spawning stress response immunosuppression energy reserve adenylate energy charge Environmental Sciences |
spellingShingle |
Oyster mortality simulated bacterial challenge spawning stress response immunosuppression energy reserve adenylate energy charge Environmental Sciences Li, Yan Qin, Jian G Li, Xiaoxu Benkendorff, Kirsten, Dr Spawning-dependent stress responses in Pacific oysters, Crassostrea gigas: a simulated bacterial challenge in oysters |
topic_facet |
Oyster mortality simulated bacterial challenge spawning stress response immunosuppression energy reserve adenylate energy charge Environmental Sciences |
description |
This study investigated the effect of spawning activity on the ability of oysters to respond to a simulated bacterial challenge, as a possible explanation for summer mortality. Using injection with a nonviable bacterial solution (NBS) of Vibrio harveyi, comparisons of immune parameters and biochemical stress responses were made between pre- and post-spawning Crassostrea gigas. Oyster mortality was also examined with extracellular products injection from V. harveyi cultures, with post-spawning oysters suffering significantly greater mortality than pre-spawning oysters. Oyster cellular immune defence to NBS was not influenced by spawning, indicated by comparable phagocytosis rates in pre- and post-spawning oysters. However, hemolymph antimicrobial activity was significantly diminished in post-spawning oysters, coincident with a reduction of hemolymph protein. The analysis of glycogen reserves demonstrated that the recovery process of post-spawning oysters was impaired by NBS challenge, resulting in a suppressed metabolic activity, indicated by lower adenylate energy charge. These results suggest that the energy cost of spawning compromises the immune and metabolic responses of oysters, leaving them more vulnerable to pathogenic challenge. |
format |
Article in Journal/Newspaper |
author |
Li, Yan Qin, Jian G Li, Xiaoxu Benkendorff, Kirsten, Dr |
author_facet |
Li, Yan Qin, Jian G Li, Xiaoxu Benkendorff, Kirsten, Dr |
author_sort |
Li, Yan |
title |
Spawning-dependent stress responses in Pacific oysters, Crassostrea gigas: a simulated bacterial challenge in oysters |
title_short |
Spawning-dependent stress responses in Pacific oysters, Crassostrea gigas: a simulated bacterial challenge in oysters |
title_full |
Spawning-dependent stress responses in Pacific oysters, Crassostrea gigas: a simulated bacterial challenge in oysters |
title_fullStr |
Spawning-dependent stress responses in Pacific oysters, Crassostrea gigas: a simulated bacterial challenge in oysters |
title_full_unstemmed |
Spawning-dependent stress responses in Pacific oysters, Crassostrea gigas: a simulated bacterial challenge in oysters |
title_sort |
spawning-dependent stress responses in pacific oysters, crassostrea gigas: a simulated bacterial challenge in oysters |
publisher |
ePublications@SCU |
publishDate |
2009 |
url |
https://epubs.scu.edu.au/esm_pubs/3581 https://doi.org/10.1016/j.aquaculture.2009.04.044 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_source |
School of Environment, Science and Engineering Papers |
op_doi |
https://doi.org/10.1016/j.aquaculture.2009.04.044 |
container_title |
Aquaculture |
container_volume |
293 |
container_issue |
3-4 |
container_start_page |
164 |
op_container_end_page |
171 |
_version_ |
1766393676330696704 |