Anti-viral gene induction is absent upon secondary challenge with double-stranded RNA in the Pacific oyster, Crassostrea gigas
Oyster farming is one of the most important aquaculture industries in the world. However, its productivity is increasingly limited by viral disease and we do not yet have management practices, such as protective vaccination, that can control these disease outbreaks. Hence, in the current study we in...
Published in: | Fish & Shellfish Immunology |
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Online Access: | https://epubs.scu.edu.au/esm_pubs/2496 https://doi.org/10.1016/j.fsi.2014.06.010 |
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ftsoutherncu:oai:epubs.scu.edu.au:esm_pubs-3509 2023-05-15T15:58:20+02:00 Anti-viral gene induction is absent upon secondary challenge with double-stranded RNA in the Pacific oyster, Crassostrea gigas Green, Timothy J Benkendorff, Kirsten Robinson, Nick Raftos, David Speck, Peter 2014-01-01T08:00:00Z https://epubs.scu.edu.au/esm_pubs/2496 https://doi.org/10.1016/j.fsi.2014.06.010 unknown ePublications@SCU School of Environment, Science and Engineering Papers Crassostrea anti-viral response immune-priming Poly I:C DNA methylation Environmental Sciences article 2014 ftsoutherncu https://doi.org/10.1016/j.fsi.2014.06.010 2019-08-06T12:55:18Z Oyster farming is one of the most important aquaculture industries in the world. However, its productivity is increasingly limited by viral disease and we do not yet have management practices, such as protective vaccination, that can control these disease outbreaks. Hence, in the current study we investigated the expression of known anti-viral genes in oysters (Crassostrea gigas) in response to primary and secondary encounter with a virus associated molecular pattern (dsRNA), and tested whether a common form of epigenetic gene regulation (DNA methylation) was associated with the expression of these anti-viral genes. Injection of dsRNA into the adductor muscle resulted in the rapid and transient expression of virus recognition receptors (TLR & MDA5), whereas several anti-viral signalling (IRF & SOC-1) and effector (PKR & viperin) genes were still up-regulated at one week post primary challenge (p < 0.05). This primary encounter with dsRNA appeared to deplete the immune system because anti-viral gene induction was absent in the gills when oysters were given a second injection of dsRNA at 1-week post-primary injection. The expression of DNA methylation genes (DNMT1, DNMT3b, TDG, TET2) and DNA methylation profiles up-stream of specific anti-viral genes (STING, SOC-1 & Viperin) did not change in response to dsRNA injection (p > 0.05). These results collectively suggest that C. gigas does not have an enhanced anti-viral gene response (immune-priming) to secondary dsRNA challenge and that the sustained up-regulation of anti-viral signalling and effector genes following primary challenge is unlikely to be associated with upstream DNA methylation levels. Article in Journal/Newspaper Crassostrea gigas Pacific oyster Southern Cross University: epublications@SCU Pacific Fish & Shellfish Immunology 39 2 492 497 |
institution |
Open Polar |
collection |
Southern Cross University: epublications@SCU |
op_collection_id |
ftsoutherncu |
language |
unknown |
topic |
Crassostrea anti-viral response immune-priming Poly I:C DNA methylation Environmental Sciences |
spellingShingle |
Crassostrea anti-viral response immune-priming Poly I:C DNA methylation Environmental Sciences Green, Timothy J Benkendorff, Kirsten Robinson, Nick Raftos, David Speck, Peter Anti-viral gene induction is absent upon secondary challenge with double-stranded RNA in the Pacific oyster, Crassostrea gigas |
topic_facet |
Crassostrea anti-viral response immune-priming Poly I:C DNA methylation Environmental Sciences |
description |
Oyster farming is one of the most important aquaculture industries in the world. However, its productivity is increasingly limited by viral disease and we do not yet have management practices, such as protective vaccination, that can control these disease outbreaks. Hence, in the current study we investigated the expression of known anti-viral genes in oysters (Crassostrea gigas) in response to primary and secondary encounter with a virus associated molecular pattern (dsRNA), and tested whether a common form of epigenetic gene regulation (DNA methylation) was associated with the expression of these anti-viral genes. Injection of dsRNA into the adductor muscle resulted in the rapid and transient expression of virus recognition receptors (TLR & MDA5), whereas several anti-viral signalling (IRF & SOC-1) and effector (PKR & viperin) genes were still up-regulated at one week post primary challenge (p < 0.05). This primary encounter with dsRNA appeared to deplete the immune system because anti-viral gene induction was absent in the gills when oysters were given a second injection of dsRNA at 1-week post-primary injection. The expression of DNA methylation genes (DNMT1, DNMT3b, TDG, TET2) and DNA methylation profiles up-stream of specific anti-viral genes (STING, SOC-1 & Viperin) did not change in response to dsRNA injection (p > 0.05). These results collectively suggest that C. gigas does not have an enhanced anti-viral gene response (immune-priming) to secondary dsRNA challenge and that the sustained up-regulation of anti-viral signalling and effector genes following primary challenge is unlikely to be associated with upstream DNA methylation levels. |
format |
Article in Journal/Newspaper |
author |
Green, Timothy J Benkendorff, Kirsten Robinson, Nick Raftos, David Speck, Peter |
author_facet |
Green, Timothy J Benkendorff, Kirsten Robinson, Nick Raftos, David Speck, Peter |
author_sort |
Green, Timothy J |
title |
Anti-viral gene induction is absent upon secondary challenge with double-stranded RNA in the Pacific oyster, Crassostrea gigas |
title_short |
Anti-viral gene induction is absent upon secondary challenge with double-stranded RNA in the Pacific oyster, Crassostrea gigas |
title_full |
Anti-viral gene induction is absent upon secondary challenge with double-stranded RNA in the Pacific oyster, Crassostrea gigas |
title_fullStr |
Anti-viral gene induction is absent upon secondary challenge with double-stranded RNA in the Pacific oyster, Crassostrea gigas |
title_full_unstemmed |
Anti-viral gene induction is absent upon secondary challenge with double-stranded RNA in the Pacific oyster, Crassostrea gigas |
title_sort |
anti-viral gene induction is absent upon secondary challenge with double-stranded rna in the pacific oyster, crassostrea gigas |
publisher |
ePublications@SCU |
publishDate |
2014 |
url |
https://epubs.scu.edu.au/esm_pubs/2496 https://doi.org/10.1016/j.fsi.2014.06.010 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
School of Environment, Science and Engineering Papers |
op_doi |
https://doi.org/10.1016/j.fsi.2014.06.010 |
container_title |
Fish & Shellfish Immunology |
container_volume |
39 |
container_issue |
2 |
container_start_page |
492 |
op_container_end_page |
497 |
_version_ |
1766394059133288448 |