Surfactant enhanced lipase containing films characterized by confocal laser scanning microscopy
Confocal laser scanning microscopy (CLSM) in combination with a fluorescently labeling enzyme dye, LavaPurple™, was demonstrated as a technique for the visualization of Thermomyces (Humicola) lanuginosa lipase (LIPHLL) and Candida antarctica lipase A (LIPCA) within a transparent latex coating. Addit...
Main Authors: | , , , , , , |
---|---|
Format: | Article in Journal/Newspaper |
Language: | unknown |
Published: |
ePublications@SCU
2011
|
Subjects: | |
Online Access: | https://epubs.scu.edu.au/esm_pubs/1086 https://epubs.scu.edu.au/cgi/viewcontent.cgi?article=2088&context=esm_pubs |
id |
ftsoutherncu:oai:epubs.scu.edu.au:esm_pubs-2088 |
---|---|
record_format |
openpolar |
spelling |
ftsoutherncu:oai:epubs.scu.edu.au:esm_pubs-2088 2023-05-15T13:47:30+02:00 Surfactant enhanced lipase containing films characterized by confocal laser scanning microscopy Jayawardena, Menuk B Yee, Lachlan H Rainbow, Ian J Bergquist, Peter Such, Christopher Steinberg, Peter D Kjelleberg, Staffan J 2011-01-01T08:00:00Z application/pdf https://epubs.scu.edu.au/esm_pubs/1086 https://epubs.scu.edu.au/cgi/viewcontent.cgi?article=2088&context=esm_pubs unknown ePublications@SCU School of Environment, Science and Engineering Papers Confocal laser scanning microscopy lipase catalytic coating surface enzyme activity surfactant latex Environmental Sciences article 2011 ftsoutherncu 2019-08-06T12:45:04Z Confocal laser scanning microscopy (CLSM) in combination with a fluorescently labeling enzyme dye, LavaPurple™, was demonstrated as a technique for the visualization of Thermomyces (Humicola) lanuginosa lipase (LIPHLL) and Candida antarctica lipase A (LIPCA) within a transparent latex coating. Addition of Teric Surfactants (C16 non-ionic Teric 475, 1.8% (w/w) or C10 non-ionic Teric 460, 2.0% (w/w)) significantly increased the accumulation of both LIPHLL and LIPCA to the surface of a latex coating. An α-naphthyl acetate substrate assay was used to quantify the accumulated lipase. The results derived from the acetate assay correlated with the enzyme accumulation (at the surface) observed in the CLSM images of the latex coating. This correlation demonstrated that the increased enzyme accumulation within the top 2 μm of the latex film was responsible for the increase in surface enzymatic activity. The combination of CLSM imagery and quantifiable image analysis provided a valuable tool for the optimization of surfactant concentrations for maximizing the activity of an enzyme (and potentially other additives) within a latex coating. Article in Journal/Newspaper Antarc* Antarctica Southern Cross University: epublications@SCU |
institution |
Open Polar |
collection |
Southern Cross University: epublications@SCU |
op_collection_id |
ftsoutherncu |
language |
unknown |
topic |
Confocal laser scanning microscopy lipase catalytic coating surface enzyme activity surfactant latex Environmental Sciences |
spellingShingle |
Confocal laser scanning microscopy lipase catalytic coating surface enzyme activity surfactant latex Environmental Sciences Jayawardena, Menuk B Yee, Lachlan H Rainbow, Ian J Bergquist, Peter Such, Christopher Steinberg, Peter D Kjelleberg, Staffan J Surfactant enhanced lipase containing films characterized by confocal laser scanning microscopy |
topic_facet |
Confocal laser scanning microscopy lipase catalytic coating surface enzyme activity surfactant latex Environmental Sciences |
description |
Confocal laser scanning microscopy (CLSM) in combination with a fluorescently labeling enzyme dye, LavaPurple™, was demonstrated as a technique for the visualization of Thermomyces (Humicola) lanuginosa lipase (LIPHLL) and Candida antarctica lipase A (LIPCA) within a transparent latex coating. Addition of Teric Surfactants (C16 non-ionic Teric 475, 1.8% (w/w) or C10 non-ionic Teric 460, 2.0% (w/w)) significantly increased the accumulation of both LIPHLL and LIPCA to the surface of a latex coating. An α-naphthyl acetate substrate assay was used to quantify the accumulated lipase. The results derived from the acetate assay correlated with the enzyme accumulation (at the surface) observed in the CLSM images of the latex coating. This correlation demonstrated that the increased enzyme accumulation within the top 2 μm of the latex film was responsible for the increase in surface enzymatic activity. The combination of CLSM imagery and quantifiable image analysis provided a valuable tool for the optimization of surfactant concentrations for maximizing the activity of an enzyme (and potentially other additives) within a latex coating. |
format |
Article in Journal/Newspaper |
author |
Jayawardena, Menuk B Yee, Lachlan H Rainbow, Ian J Bergquist, Peter Such, Christopher Steinberg, Peter D Kjelleberg, Staffan J |
author_facet |
Jayawardena, Menuk B Yee, Lachlan H Rainbow, Ian J Bergquist, Peter Such, Christopher Steinberg, Peter D Kjelleberg, Staffan J |
author_sort |
Jayawardena, Menuk B |
title |
Surfactant enhanced lipase containing films characterized by confocal laser scanning microscopy |
title_short |
Surfactant enhanced lipase containing films characterized by confocal laser scanning microscopy |
title_full |
Surfactant enhanced lipase containing films characterized by confocal laser scanning microscopy |
title_fullStr |
Surfactant enhanced lipase containing films characterized by confocal laser scanning microscopy |
title_full_unstemmed |
Surfactant enhanced lipase containing films characterized by confocal laser scanning microscopy |
title_sort |
surfactant enhanced lipase containing films characterized by confocal laser scanning microscopy |
publisher |
ePublications@SCU |
publishDate |
2011 |
url |
https://epubs.scu.edu.au/esm_pubs/1086 https://epubs.scu.edu.au/cgi/viewcontent.cgi?article=2088&context=esm_pubs |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_source |
School of Environment, Science and Engineering Papers |
_version_ |
1766247243588829184 |