Summary: | While there are extensive macro- and microfossil records of a range of plants and animals from Quaternary records, earthworms and their close relatives among annelids are not preserved as fossils, and therefore we have limited knowledge of their Quaternary distributions. This lack of fossils means that clitellate worms (Annelida) are currently underused in palaeoecological research, even though they can provide valuable information about terrestrial and aquatic environmental conditions. Their DNA might be preserved in sediments, which offers an alternative method for detection. Here we analyse lacustrine sediments from lakes in the Polar Urals, Arctic Russia, covering the period 24,000-1,300 cal. years BP, and NE Norway (10,700-3,300 cal. years BP) using a universal mammal 16S rDNA marker. While mammals were recorded using the marker (reindeer was detected twice in the Polar Urals core at 23,000 and 14,000 cal. years BP, and four times in the Norwegian core at 11,000 cal. years BP and between 3,600-3,300 cal. years BP), worm extracellular DNA “bycatch” was rather high. In this paper we present the first reported worm detection from ancient DNA. Our results demonstrate that both aquatic and terrestrial clitellates can be identified in late-Quaternary lacustrine sediments, and the ecological information retrievable from this group warrants further research with a more targeted approach.,FilterBarcodingR script used for the Filtering of the OBITOOLS output.Sample_tag_primer_URAL-MamExcel file containing the primer tags and sample names for the Polar Urals run. The primer information was used by OBITOOLS to demultiplex the data.Sample_tag_primer_Varanger-MamP007Excel file containing the primer tags and sample names for the Varanger run. The primer information was used by OBITOOLS to demultiplex the data.URAL-Mam.un.an.C1.L13.obiclean.r5.HpS.M-iden.ann.sort_10_23_1_2_1The filtered OBITOOLS output for the Polar Urals run.Hubia2-Mammal.ali.uniq.c2.cl.M-iden.ann.sort_10_23_1_2_1The filtered Varanger OBITOOLS ...
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