Metabarcoding of modern soil DNA gives a highly local vegetation signal in Svalbard tundra
Environmental DNA retrieved from modern soils (eDNA) and late-Quaternary palaeosols and sediments (aDNA and sedaDNA) promises insight into the composition of present and past terrestrial biotic communities, but few studies address the spatial relationship between recovered eDNA and contributing orga...
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Online Access: | https://eprints.soton.ac.uk/425807/ https://eprints.soton.ac.uk/425807/1/Edwards_et_al_Svalbard_DNA_revised_preprint_for_authors.pdf |
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ftsouthampton:oai:eprints.soton.ac.uk:425807 2023-07-30T04:07:11+02:00 Metabarcoding of modern soil DNA gives a highly local vegetation signal in Svalbard tundra Edwards, Mary Alsos, Inger Greve Yoccoz, Nigel Giles Coissac, Eric Goslar, Tomasz Gielly, Ludovic Haile, James Langdon, Catherine Tribsch, Andreas Binney, Heather Von Stedingk, Henrik Taberlet, Pierre 2018-09-10 text https://eprints.soton.ac.uk/425807/ https://eprints.soton.ac.uk/425807/1/Edwards_et_al_Svalbard_DNA_revised_preprint_for_authors.pdf en English eng https://eprints.soton.ac.uk/425807/1/Edwards_et_al_Svalbard_DNA_revised_preprint_for_authors.pdf Edwards, Mary, Alsos, Inger Greve, Yoccoz, Nigel Giles, Coissac, Eric, Goslar, Tomasz, Gielly, Ludovic, Haile, James, Langdon, Catherine, Tribsch, Andreas, Binney, Heather, Von Stedingk, Henrik and Taberlet, Pierre (2018) Metabarcoding of modern soil DNA gives a highly local vegetation signal in Svalbard tundra. The Holocene. (doi:10.1177/0959683618798095 <http://dx.doi.org/10.1177/0959683618798095>). accepted_manuscript Article PeerReviewed 2018 ftsouthampton https://doi.org/10.1177/0959683618798095 2023-07-09T22:26:03Z Environmental DNA retrieved from modern soils (eDNA) and late-Quaternary palaeosols and sediments (aDNA and sedaDNA) promises insight into the composition of present and past terrestrial biotic communities, but few studies address the spatial relationship between recovered eDNA and contributing organisms. Svalbard’s vascular plant flora is well known, and a cold climate enhances preservation of eDNA in soils. Thus, Svalbard plant communities are excellent systems for addressing the representation of plant eDNA in soil samples. In two valleys in the inner fjord region of Spitsbergen, we carried out detailed vegetation surveys of circular plots up to a 4-m radius. One or three near-surface soil samples from each plot were used for extraction and metabarcoding of soil-derived eDNA. Use of PCR replicates and appropriate filtering, plus a relevant reference metabarcode catalogue, provided taxon lists that reflected the local flora. There was high concordance between taxa recorded in plot vegetation and those in the eDNA, but floristic diversity was under-sampled, even at the scale of a 1-m radius plot. Most detected taxa grew within < 0.5–1.0 m of the sampling point. Taxa present in vegetation but not in eDNA tended to occur further from the sampling point, and most had above-ground cover of < 5%. Soil-derived eDNA provides a highly local floristic signal, and this spatial constraint should be considered in sampling designs. For palaeoecological or archaeological studies, multiple samples from a given soil horizon that are spatially distributed across the area of interest are likely to provide the most complete picture of species presence. Article in Journal/Newspaper Svalbard Tundra Spitsbergen University of Southampton: e-Prints Soton Svalbard The Holocene 28 12 2006 2016 |
institution |
Open Polar |
collection |
University of Southampton: e-Prints Soton |
op_collection_id |
ftsouthampton |
language |
English |
description |
Environmental DNA retrieved from modern soils (eDNA) and late-Quaternary palaeosols and sediments (aDNA and sedaDNA) promises insight into the composition of present and past terrestrial biotic communities, but few studies address the spatial relationship between recovered eDNA and contributing organisms. Svalbard’s vascular plant flora is well known, and a cold climate enhances preservation of eDNA in soils. Thus, Svalbard plant communities are excellent systems for addressing the representation of plant eDNA in soil samples. In two valleys in the inner fjord region of Spitsbergen, we carried out detailed vegetation surveys of circular plots up to a 4-m radius. One or three near-surface soil samples from each plot were used for extraction and metabarcoding of soil-derived eDNA. Use of PCR replicates and appropriate filtering, plus a relevant reference metabarcode catalogue, provided taxon lists that reflected the local flora. There was high concordance between taxa recorded in plot vegetation and those in the eDNA, but floristic diversity was under-sampled, even at the scale of a 1-m radius plot. Most detected taxa grew within < 0.5–1.0 m of the sampling point. Taxa present in vegetation but not in eDNA tended to occur further from the sampling point, and most had above-ground cover of < 5%. Soil-derived eDNA provides a highly local floristic signal, and this spatial constraint should be considered in sampling designs. For palaeoecological or archaeological studies, multiple samples from a given soil horizon that are spatially distributed across the area of interest are likely to provide the most complete picture of species presence. |
format |
Article in Journal/Newspaper |
author |
Edwards, Mary Alsos, Inger Greve Yoccoz, Nigel Giles Coissac, Eric Goslar, Tomasz Gielly, Ludovic Haile, James Langdon, Catherine Tribsch, Andreas Binney, Heather Von Stedingk, Henrik Taberlet, Pierre |
spellingShingle |
Edwards, Mary Alsos, Inger Greve Yoccoz, Nigel Giles Coissac, Eric Goslar, Tomasz Gielly, Ludovic Haile, James Langdon, Catherine Tribsch, Andreas Binney, Heather Von Stedingk, Henrik Taberlet, Pierre Metabarcoding of modern soil DNA gives a highly local vegetation signal in Svalbard tundra |
author_facet |
Edwards, Mary Alsos, Inger Greve Yoccoz, Nigel Giles Coissac, Eric Goslar, Tomasz Gielly, Ludovic Haile, James Langdon, Catherine Tribsch, Andreas Binney, Heather Von Stedingk, Henrik Taberlet, Pierre |
author_sort |
Edwards, Mary |
title |
Metabarcoding of modern soil DNA gives a highly local vegetation signal in Svalbard tundra |
title_short |
Metabarcoding of modern soil DNA gives a highly local vegetation signal in Svalbard tundra |
title_full |
Metabarcoding of modern soil DNA gives a highly local vegetation signal in Svalbard tundra |
title_fullStr |
Metabarcoding of modern soil DNA gives a highly local vegetation signal in Svalbard tundra |
title_full_unstemmed |
Metabarcoding of modern soil DNA gives a highly local vegetation signal in Svalbard tundra |
title_sort |
metabarcoding of modern soil dna gives a highly local vegetation signal in svalbard tundra |
publishDate |
2018 |
url |
https://eprints.soton.ac.uk/425807/ https://eprints.soton.ac.uk/425807/1/Edwards_et_al_Svalbard_DNA_revised_preprint_for_authors.pdf |
geographic |
Svalbard |
geographic_facet |
Svalbard |
genre |
Svalbard Tundra Spitsbergen |
genre_facet |
Svalbard Tundra Spitsbergen |
op_relation |
https://eprints.soton.ac.uk/425807/1/Edwards_et_al_Svalbard_DNA_revised_preprint_for_authors.pdf Edwards, Mary, Alsos, Inger Greve, Yoccoz, Nigel Giles, Coissac, Eric, Goslar, Tomasz, Gielly, Ludovic, Haile, James, Langdon, Catherine, Tribsch, Andreas, Binney, Heather, Von Stedingk, Henrik and Taberlet, Pierre (2018) Metabarcoding of modern soil DNA gives a highly local vegetation signal in Svalbard tundra. The Holocene. (doi:10.1177/0959683618798095 <http://dx.doi.org/10.1177/0959683618798095>). |
op_rights |
accepted_manuscript |
op_doi |
https://doi.org/10.1177/0959683618798095 |
container_title |
The Holocene |
container_volume |
28 |
container_issue |
12 |
container_start_page |
2006 |
op_container_end_page |
2016 |
_version_ |
1772820341216772096 |