Nitrogen fixation and nitrogenase (nifH) expression in tropical waters of the eastern North Atlantic
Expression of nifH in 28 surface water samples collected during fall 2007 from six stations in the vicinity of the Cape Verde Islands (north-east Atlantic) was examined using reverse transcription-polymerase chain reaction (RT-PCR)-based clone libraries and quantitative RT-PCR (RT-qPCR) analysis of...
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2011
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ftsouthampton:oai:eprints.soton.ac.uk:414023 2023-07-30T04:05:29+02:00 Nitrogen fixation and nitrogenase (nifH) expression in tropical waters of the eastern North Atlantic Turk, Kendra A. Rees, Andrew P. Zehr, Jonathan P. Pereira, Nicole Swift, Paul Shelley, Rachel Lohan, Maeve Woodward, E. Malcolm S Gilbert, Jack 2011-07 https://eprints.soton.ac.uk/414023/ English eng Turk, Kendra A., Rees, Andrew P., Zehr, Jonathan P., Pereira, Nicole, Swift, Paul, Shelley, Rachel, Lohan, Maeve, Woodward, E. Malcolm S and Gilbert, Jack (2011) Nitrogen fixation and nitrogenase (nifH) expression in tropical waters of the eastern North Atlantic. The ISME Journal, 5 (7), 1201-1212. (doi:10.1038/ismej.2010.205 <http://dx.doi.org/10.1038/ismej.2010.205>). Article PeerReviewed 2011 ftsouthampton https://doi.org/10.1038/ismej.2010.205 2023-07-09T22:17:43Z Expression of nifH in 28 surface water samples collected during fall 2007 from six stations in the vicinity of the Cape Verde Islands (north-east Atlantic) was examined using reverse transcription-polymerase chain reaction (RT-PCR)-based clone libraries and quantitative RT-PCR (RT-qPCR) analysis of seven diazotrophic phylotypes. Biological nitrogen fixation (BNF) rates and nutrient concentrations were determined for these stations, which were selected based on a range in surface chlorophyll concentrations to target a gradient of primary productivity. BNF rates greater than 6 nmolNl -1 h -1 were measured at two of the near-shore stations where high concentrations of Fe and PO 4 3- were also measured. Six hundred and five nifH transcripts were amplified by RT-PCR, of which 76% are described by six operational taxonomic units, including Trichodesmium and the uncultivated UCYN-A, and four non-cyanobacterial diazotrophs that clustered with uncultivated Proteobacteria. Although all five cyanobacterial phylotypes quantified in RT-qPCR assays were detected at different stations in this study, UCYN-A contributed most significantly to the pool of nifH transcripts in both coastal and oligotrophic waters. A comparison of results from RT-PCR clone libraries and RT-qPCR indicated that a γ-proteobacterial phylotype was preferentially amplified in clone libraries, which underscores the need to use caution interpreting clone-library-based nifH studies, especially when considering the importance of uncultivated proteobacterial diazotrophs. Article in Journal/Newspaper North Atlantic North East Atlantic University of Southampton: e-Prints Soton The ISME Journal 5 7 1201 1212 |
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Open Polar |
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University of Southampton: e-Prints Soton |
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ftsouthampton |
language |
English |
description |
Expression of nifH in 28 surface water samples collected during fall 2007 from six stations in the vicinity of the Cape Verde Islands (north-east Atlantic) was examined using reverse transcription-polymerase chain reaction (RT-PCR)-based clone libraries and quantitative RT-PCR (RT-qPCR) analysis of seven diazotrophic phylotypes. Biological nitrogen fixation (BNF) rates and nutrient concentrations were determined for these stations, which were selected based on a range in surface chlorophyll concentrations to target a gradient of primary productivity. BNF rates greater than 6 nmolNl -1 h -1 were measured at two of the near-shore stations where high concentrations of Fe and PO 4 3- were also measured. Six hundred and five nifH transcripts were amplified by RT-PCR, of which 76% are described by six operational taxonomic units, including Trichodesmium and the uncultivated UCYN-A, and four non-cyanobacterial diazotrophs that clustered with uncultivated Proteobacteria. Although all five cyanobacterial phylotypes quantified in RT-qPCR assays were detected at different stations in this study, UCYN-A contributed most significantly to the pool of nifH transcripts in both coastal and oligotrophic waters. A comparison of results from RT-PCR clone libraries and RT-qPCR indicated that a γ-proteobacterial phylotype was preferentially amplified in clone libraries, which underscores the need to use caution interpreting clone-library-based nifH studies, especially when considering the importance of uncultivated proteobacterial diazotrophs. |
format |
Article in Journal/Newspaper |
author |
Turk, Kendra A. Rees, Andrew P. Zehr, Jonathan P. Pereira, Nicole Swift, Paul Shelley, Rachel Lohan, Maeve Woodward, E. Malcolm S Gilbert, Jack |
spellingShingle |
Turk, Kendra A. Rees, Andrew P. Zehr, Jonathan P. Pereira, Nicole Swift, Paul Shelley, Rachel Lohan, Maeve Woodward, E. Malcolm S Gilbert, Jack Nitrogen fixation and nitrogenase (nifH) expression in tropical waters of the eastern North Atlantic |
author_facet |
Turk, Kendra A. Rees, Andrew P. Zehr, Jonathan P. Pereira, Nicole Swift, Paul Shelley, Rachel Lohan, Maeve Woodward, E. Malcolm S Gilbert, Jack |
author_sort |
Turk, Kendra A. |
title |
Nitrogen fixation and nitrogenase (nifH) expression in tropical waters of the eastern North Atlantic |
title_short |
Nitrogen fixation and nitrogenase (nifH) expression in tropical waters of the eastern North Atlantic |
title_full |
Nitrogen fixation and nitrogenase (nifH) expression in tropical waters of the eastern North Atlantic |
title_fullStr |
Nitrogen fixation and nitrogenase (nifH) expression in tropical waters of the eastern North Atlantic |
title_full_unstemmed |
Nitrogen fixation and nitrogenase (nifH) expression in tropical waters of the eastern North Atlantic |
title_sort |
nitrogen fixation and nitrogenase (nifh) expression in tropical waters of the eastern north atlantic |
publishDate |
2011 |
url |
https://eprints.soton.ac.uk/414023/ |
genre |
North Atlantic North East Atlantic |
genre_facet |
North Atlantic North East Atlantic |
op_relation |
Turk, Kendra A., Rees, Andrew P., Zehr, Jonathan P., Pereira, Nicole, Swift, Paul, Shelley, Rachel, Lohan, Maeve, Woodward, E. Malcolm S and Gilbert, Jack (2011) Nitrogen fixation and nitrogenase (nifH) expression in tropical waters of the eastern North Atlantic. The ISME Journal, 5 (7), 1201-1212. (doi:10.1038/ismej.2010.205 <http://dx.doi.org/10.1038/ismej.2010.205>). |
op_doi |
https://doi.org/10.1038/ismej.2010.205 |
container_title |
The ISME Journal |
container_volume |
5 |
container_issue |
7 |
container_start_page |
1201 |
op_container_end_page |
1212 |
_version_ |
1772817439750356992 |