A multi-parametric assessment of decontamination protocols for the subglacial Lake Ellsworth probe
Direct measurement and sampling of pristine environments, such as subglacial lakes, without introducing contaminating microorganisms and biomolecules from the surface, represents a significant engineering and microbiological challenge. In this study, we compare methods for decontamination of titaniu...
Published in: | Journal of Microbiological Methods |
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ftsouthampton:oai:eprints.soton.ac.uk:390964 2023-07-30T03:57:15+02:00 A multi-parametric assessment of decontamination protocols for the subglacial Lake Ellsworth probe Magiopoulos, I. Mcquillan, J.S. Burd, C.L. Mowlem, M. Tsaloglou, M.-N. 2016-04 https://eprints.soton.ac.uk/390964/ English eng Magiopoulos, I., Mcquillan, J.S., Burd, C.L., Mowlem, M. and Tsaloglou, M.-N. (2016) A multi-parametric assessment of decontamination protocols for the subglacial Lake Ellsworth probe. Journal of Microbiological Methods, 123, 87-93. (doi:10.1016/j.mimet.2016.02.012 <http://dx.doi.org/10.1016/j.mimet.2016.02.012>). Article PeerReviewed 2016 ftsouthampton https://doi.org/10.1016/j.mimet.2016.02.012 2023-07-09T22:05:50Z Direct measurement and sampling of pristine environments, such as subglacial lakes, without introducing contaminating microorganisms and biomolecules from the surface, represents a significant engineering and microbiological challenge. In this study, we compare methods for decontamination of titanium grade 5 surfaces, the material extensively used to construct a custom-made probe for reaching, measuring and sampling subglacial Lake Ellsworth in West Antarctica. Coupons of titanium were artificially contaminated with Pseudomonas fluorescens bacteria and then exposed to a number of decontamination procedures. The most effective sterilants were (i) hydrogen peroxide vapour, and (ii) Biocleanseâ„¢, a commercially available, detergent-based biocidal solution. After each decontamination procedure the bacteria were incapable of proliferation, and showed no evidence of metabolic activity based on the generation of adenosine triphosphate (ATP). The use of ultraviolet irradiation or ethyl alcohol solution was comparatively ineffective for sterilisation. Hydrogen peroxide vapour and ultraviolet irradiation, which directly damage nucleic acids, were the most effective methods for removing detectable DNA, which was measured using 16S rRNA gene copy number and fluorescence-based total DNA quantification. Our results have not only been used to tailor the Ellsworth probe decontamination process, but also hold value for subsequent engineering projects, where high standards of decontamination are required. Article in Journal/Newspaper Antarc* Antarctica West Antarctica University of Southampton: e-Prints Soton West Antarctica Journal of Microbiological Methods 123 87 93 |
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University of Southampton: e-Prints Soton |
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ftsouthampton |
language |
English |
description |
Direct measurement and sampling of pristine environments, such as subglacial lakes, without introducing contaminating microorganisms and biomolecules from the surface, represents a significant engineering and microbiological challenge. In this study, we compare methods for decontamination of titanium grade 5 surfaces, the material extensively used to construct a custom-made probe for reaching, measuring and sampling subglacial Lake Ellsworth in West Antarctica. Coupons of titanium were artificially contaminated with Pseudomonas fluorescens bacteria and then exposed to a number of decontamination procedures. The most effective sterilants were (i) hydrogen peroxide vapour, and (ii) Biocleanseâ„¢, a commercially available, detergent-based biocidal solution. After each decontamination procedure the bacteria were incapable of proliferation, and showed no evidence of metabolic activity based on the generation of adenosine triphosphate (ATP). The use of ultraviolet irradiation or ethyl alcohol solution was comparatively ineffective for sterilisation. Hydrogen peroxide vapour and ultraviolet irradiation, which directly damage nucleic acids, were the most effective methods for removing detectable DNA, which was measured using 16S rRNA gene copy number and fluorescence-based total DNA quantification. Our results have not only been used to tailor the Ellsworth probe decontamination process, but also hold value for subsequent engineering projects, where high standards of decontamination are required. |
format |
Article in Journal/Newspaper |
author |
Magiopoulos, I. Mcquillan, J.S. Burd, C.L. Mowlem, M. Tsaloglou, M.-N. |
spellingShingle |
Magiopoulos, I. Mcquillan, J.S. Burd, C.L. Mowlem, M. Tsaloglou, M.-N. A multi-parametric assessment of decontamination protocols for the subglacial Lake Ellsworth probe |
author_facet |
Magiopoulos, I. Mcquillan, J.S. Burd, C.L. Mowlem, M. Tsaloglou, M.-N. |
author_sort |
Magiopoulos, I. |
title |
A multi-parametric assessment of decontamination protocols for the subglacial Lake Ellsworth probe |
title_short |
A multi-parametric assessment of decontamination protocols for the subglacial Lake Ellsworth probe |
title_full |
A multi-parametric assessment of decontamination protocols for the subglacial Lake Ellsworth probe |
title_fullStr |
A multi-parametric assessment of decontamination protocols for the subglacial Lake Ellsworth probe |
title_full_unstemmed |
A multi-parametric assessment of decontamination protocols for the subglacial Lake Ellsworth probe |
title_sort |
multi-parametric assessment of decontamination protocols for the subglacial lake ellsworth probe |
publishDate |
2016 |
url |
https://eprints.soton.ac.uk/390964/ |
geographic |
West Antarctica |
geographic_facet |
West Antarctica |
genre |
Antarc* Antarctica West Antarctica |
genre_facet |
Antarc* Antarctica West Antarctica |
op_relation |
Magiopoulos, I., Mcquillan, J.S., Burd, C.L., Mowlem, M. and Tsaloglou, M.-N. (2016) A multi-parametric assessment of decontamination protocols for the subglacial Lake Ellsworth probe. Journal of Microbiological Methods, 123, 87-93. (doi:10.1016/j.mimet.2016.02.012 <http://dx.doi.org/10.1016/j.mimet.2016.02.012>). |
op_doi |
https://doi.org/10.1016/j.mimet.2016.02.012 |
container_title |
Journal of Microbiological Methods |
container_volume |
123 |
container_start_page |
87 |
op_container_end_page |
93 |
_version_ |
1772816549575393280 |