Dominant oceanic bacteria secure phosphate using a large extracellular buffer
The ubiquitous SAR11 and Prochlorococcus bacteria manage to maintain a sufficient supply of phosphate in phosphate-poor surface waters of the North Atlantic subtropical gyre. Furthermore, it seems that their phosphate uptake may counter-intuitively be lower in more productive tropical waters, as if...
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ftsouthampton:oai:eprints.soton.ac.uk:378220 2023-07-30T04:05:24+02:00 Dominant oceanic bacteria secure phosphate using a large extracellular buffer Zubkov, M.V. Martin, A.P. Hartmann, M. Grob, C. Scanlan, D.J. 2015-07-22 text https://eprints.soton.ac.uk/378220/ https://eprints.soton.ac.uk/378220/1/NCOMMS-15-00997A_R2-1_for%2520library.pdf https://eprints.soton.ac.uk/378220/2/NCOMMS-15-00997A_Suppl_R2.pdf https://eprints.soton.ac.uk/378220/3/ncomms8878.pdf en English eng https://eprints.soton.ac.uk/378220/1/NCOMMS-15-00997A_R2-1_for%2520library.pdf https://eprints.soton.ac.uk/378220/2/NCOMMS-15-00997A_Suppl_R2.pdf https://eprints.soton.ac.uk/378220/3/ncomms8878.pdf Zubkov, M.V., Martin, A.P., Hartmann, M., Grob, C. and Scanlan, D.J. (2015) Dominant oceanic bacteria secure phosphate using a large extracellular buffer. Nature Communications, 6, 7878. cc_by_4 Article NonPeerReviewed 2015 ftsouthampton 2023-07-09T21:59:41Z The ubiquitous SAR11 and Prochlorococcus bacteria manage to maintain a sufficient supply of phosphate in phosphate-poor surface waters of the North Atlantic subtropical gyre. Furthermore, it seems that their phosphate uptake may counter-intuitively be lower in more productive tropical waters, as if their cellular demand for phosphate decreases there. By flow sorting 33P-phosphate-pulsed 32P-phosphate-chased cells, we demonstrate that both Prochlorococcus and SAR11 cells exploit an extracellular buffer of labile phosphate up to 5–40 times larger than the amount of phosphate required to replicate their chromosomes. Mathematical modelling is shown to support this conclusion. The fuller the buffer the slower the cellular uptake of phosphate, to the point that in phosphate-replete tropical waters, cells can saturate their buffer and their phosphate uptake becomes marginal. Hence, buffer stocking is a generic, growth-securing adaptation for SAR11 and Prochlorococcus bacteria, which lack internal reserves to reduce their dependency on bioavailable ambient phosphate. Article in Journal/Newspaper North Atlantic University of Southampton: e-Prints Soton Fuller ENVELOPE(162.350,162.350,-77.867,-77.867) |
institution |
Open Polar |
collection |
University of Southampton: e-Prints Soton |
op_collection_id |
ftsouthampton |
language |
English |
description |
The ubiquitous SAR11 and Prochlorococcus bacteria manage to maintain a sufficient supply of phosphate in phosphate-poor surface waters of the North Atlantic subtropical gyre. Furthermore, it seems that their phosphate uptake may counter-intuitively be lower in more productive tropical waters, as if their cellular demand for phosphate decreases there. By flow sorting 33P-phosphate-pulsed 32P-phosphate-chased cells, we demonstrate that both Prochlorococcus and SAR11 cells exploit an extracellular buffer of labile phosphate up to 5–40 times larger than the amount of phosphate required to replicate their chromosomes. Mathematical modelling is shown to support this conclusion. The fuller the buffer the slower the cellular uptake of phosphate, to the point that in phosphate-replete tropical waters, cells can saturate their buffer and their phosphate uptake becomes marginal. Hence, buffer stocking is a generic, growth-securing adaptation for SAR11 and Prochlorococcus bacteria, which lack internal reserves to reduce their dependency on bioavailable ambient phosphate. |
format |
Article in Journal/Newspaper |
author |
Zubkov, M.V. Martin, A.P. Hartmann, M. Grob, C. Scanlan, D.J. |
spellingShingle |
Zubkov, M.V. Martin, A.P. Hartmann, M. Grob, C. Scanlan, D.J. Dominant oceanic bacteria secure phosphate using a large extracellular buffer |
author_facet |
Zubkov, M.V. Martin, A.P. Hartmann, M. Grob, C. Scanlan, D.J. |
author_sort |
Zubkov, M.V. |
title |
Dominant oceanic bacteria secure phosphate using a large extracellular buffer |
title_short |
Dominant oceanic bacteria secure phosphate using a large extracellular buffer |
title_full |
Dominant oceanic bacteria secure phosphate using a large extracellular buffer |
title_fullStr |
Dominant oceanic bacteria secure phosphate using a large extracellular buffer |
title_full_unstemmed |
Dominant oceanic bacteria secure phosphate using a large extracellular buffer |
title_sort |
dominant oceanic bacteria secure phosphate using a large extracellular buffer |
publishDate |
2015 |
url |
https://eprints.soton.ac.uk/378220/ https://eprints.soton.ac.uk/378220/1/NCOMMS-15-00997A_R2-1_for%2520library.pdf https://eprints.soton.ac.uk/378220/2/NCOMMS-15-00997A_Suppl_R2.pdf https://eprints.soton.ac.uk/378220/3/ncomms8878.pdf |
long_lat |
ENVELOPE(162.350,162.350,-77.867,-77.867) |
geographic |
Fuller |
geographic_facet |
Fuller |
genre |
North Atlantic |
genre_facet |
North Atlantic |
op_relation |
https://eprints.soton.ac.uk/378220/1/NCOMMS-15-00997A_R2-1_for%2520library.pdf https://eprints.soton.ac.uk/378220/2/NCOMMS-15-00997A_Suppl_R2.pdf https://eprints.soton.ac.uk/378220/3/ncomms8878.pdf Zubkov, M.V., Martin, A.P., Hartmann, M., Grob, C. and Scanlan, D.J. (2015) Dominant oceanic bacteria secure phosphate using a large extracellular buffer. Nature Communications, 6, 7878. |
op_rights |
cc_by_4 |
_version_ |
1772817285458690048 |