Flow cytometric identification of Mamiellales clade II in the Southern Atlantic Ocean
Flow cytometric sorting, based on cellular optical properties and macromolecule content, has been successfully employed to taxonomically affiliate bacterioplankton. However, this approach has not been much used for eukaryotic plankton. To redress this imbalance, we identified a conspicuous group of...
Published in: | FEMS Microbiology Ecology |
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ftsouthampton:oai:eprints.soton.ac.uk:348336 2023-07-30T04:06:50+02:00 Flow cytometric identification of Mamiellales clade II in the Southern Atlantic Ocean Gómez-Pereira, Paola R. Kennaway, Gabrielle Fuchs, Bernhard M. Tarran, Glen A. Zubkov, Mikhail V. 2013-03 https://eprints.soton.ac.uk/348336/ unknown Gómez-Pereira, Paola R., Kennaway, Gabrielle, Fuchs, Bernhard M., Tarran, Glen A. and Zubkov, Mikhail V. (2013) Flow cytometric identification of Mamiellales clade II in the Southern Atlantic Ocean. FEMS Microbiology Ecology, 83 (3), 664-671. (doi:10.1111/1574-6941.12023 <http://dx.doi.org/10.1111/1574-6941.12023>). Article PeerReviewed 2013 ftsouthampton https://doi.org/10.1111/1574-6941.12023 2023-07-09T21:44:12Z Flow cytometric sorting, based on cellular optical properties and macromolecule content, has been successfully employed to taxonomically affiliate bacterioplankton. However, this approach has not been much used for eukaryotic plankton. To redress this imbalance, we identified a conspicuous group of red autofluorescent picoplankton in surface waters of the South Atlantic Ocean. Using catalysed reporter deposition fluorescence in situ hybridization, virtually, all cells sorted from that group were affiliated with the Mamiellales clade II (84 ± 4%, division Chlorophyta) with a size of 1.6 ± 0.03 µm. Based on electron microscopy, the Mamiellales clade II–sorted cells have a simple morphology with apparently no scales, flagella or surface features. Their latitudinal distribution resembled the distribution of Synechococcus with very low concentrations in the surface waters of the Southern subtropical gyre (0.6–1.6 × 10 3 cells mL -1 ) and increased concentrations in the Southern temperate waters 8.3 × 10 3 cells mL -1 . Identification of the flow cytometric group as Mamiellales clade II allowed us to characterize the morphology of these enigmatic uncultured picoplanktonic cells by electron microscopy and to determine their apparent preference for temperate rather than subtropical oceanic photic waters. Article in Journal/Newspaper South Atlantic Ocean University of Southampton: e-Prints Soton FEMS Microbiology Ecology 83 3 664 671 |
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Open Polar |
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University of Southampton: e-Prints Soton |
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ftsouthampton |
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description |
Flow cytometric sorting, based on cellular optical properties and macromolecule content, has been successfully employed to taxonomically affiliate bacterioplankton. However, this approach has not been much used for eukaryotic plankton. To redress this imbalance, we identified a conspicuous group of red autofluorescent picoplankton in surface waters of the South Atlantic Ocean. Using catalysed reporter deposition fluorescence in situ hybridization, virtually, all cells sorted from that group were affiliated with the Mamiellales clade II (84 ± 4%, division Chlorophyta) with a size of 1.6 ± 0.03 µm. Based on electron microscopy, the Mamiellales clade II–sorted cells have a simple morphology with apparently no scales, flagella or surface features. Their latitudinal distribution resembled the distribution of Synechococcus with very low concentrations in the surface waters of the Southern subtropical gyre (0.6–1.6 × 10 3 cells mL -1 ) and increased concentrations in the Southern temperate waters 8.3 × 10 3 cells mL -1 . Identification of the flow cytometric group as Mamiellales clade II allowed us to characterize the morphology of these enigmatic uncultured picoplanktonic cells by electron microscopy and to determine their apparent preference for temperate rather than subtropical oceanic photic waters. |
format |
Article in Journal/Newspaper |
author |
Gómez-Pereira, Paola R. Kennaway, Gabrielle Fuchs, Bernhard M. Tarran, Glen A. Zubkov, Mikhail V. |
spellingShingle |
Gómez-Pereira, Paola R. Kennaway, Gabrielle Fuchs, Bernhard M. Tarran, Glen A. Zubkov, Mikhail V. Flow cytometric identification of Mamiellales clade II in the Southern Atlantic Ocean |
author_facet |
Gómez-Pereira, Paola R. Kennaway, Gabrielle Fuchs, Bernhard M. Tarran, Glen A. Zubkov, Mikhail V. |
author_sort |
Gómez-Pereira, Paola R. |
title |
Flow cytometric identification of Mamiellales clade II in the Southern Atlantic Ocean |
title_short |
Flow cytometric identification of Mamiellales clade II in the Southern Atlantic Ocean |
title_full |
Flow cytometric identification of Mamiellales clade II in the Southern Atlantic Ocean |
title_fullStr |
Flow cytometric identification of Mamiellales clade II in the Southern Atlantic Ocean |
title_full_unstemmed |
Flow cytometric identification of Mamiellales clade II in the Southern Atlantic Ocean |
title_sort |
flow cytometric identification of mamiellales clade ii in the southern atlantic ocean |
publishDate |
2013 |
url |
https://eprints.soton.ac.uk/348336/ |
genre |
South Atlantic Ocean |
genre_facet |
South Atlantic Ocean |
op_relation |
Gómez-Pereira, Paola R., Kennaway, Gabrielle, Fuchs, Bernhard M., Tarran, Glen A. and Zubkov, Mikhail V. (2013) Flow cytometric identification of Mamiellales clade II in the Southern Atlantic Ocean. FEMS Microbiology Ecology, 83 (3), 664-671. (doi:10.1111/1574-6941.12023 <http://dx.doi.org/10.1111/1574-6941.12023>). |
op_doi |
https://doi.org/10.1111/1574-6941.12023 |
container_title |
FEMS Microbiology Ecology |
container_volume |
83 |
container_issue |
3 |
container_start_page |
664 |
op_container_end_page |
671 |
_version_ |
1772819769499582464 |