The composition of pulmonary surfactant from diving mammals
Maintaining a functional pulmonary surfactant system at depth is critical for diving mammals to ensure that inspiration is possible upon re-emergence. The lipid and protein composition of lavage extracts from three pinniped species (California sea lion, Northern elephant seal and Ringed seal) were c...
Main Authors: | , , , , |
---|---|
Format: | Article in Journal/Newspaper |
Language: | unknown |
Published: |
2006
|
Subjects: | |
Online Access: | https://eprints.soton.ac.uk/27270/ |
id |
ftsouthampton:oai:eprints.soton.ac.uk:27270 |
---|---|
record_format |
openpolar |
spelling |
ftsouthampton:oai:eprints.soton.ac.uk:27270 2023-07-30T04:03:16+02:00 The composition of pulmonary surfactant from diving mammals Miller, Natalie J. Postle, Anthony D. Orgeig, Sandra Koster, Grielof Daniels, Christopher B. 2006 https://eprints.soton.ac.uk/27270/ unknown Miller, Natalie J., Postle, Anthony D., Orgeig, Sandra, Koster, Grielof and Daniels, Christopher B. (2006) The composition of pulmonary surfactant from diving mammals. Respiratory Physiology & Neurobiology, 152 (2), 152-168. (doi:10.1016/j.resp.2005.08.001 <http://dx.doi.org/10.1016/j.resp.2005.08.001>). Article PeerReviewed 2006 ftsouthampton 2023-07-09T20:41:26Z Maintaining a functional pulmonary surfactant system at depth is critical for diving mammals to ensure that inspiration is possible upon re-emergence. The lipid and protein composition of lavage extracts from three pinniped species (California sea lion, Northern elephant seal and Ringed seal) were compared to several terrestrial species. Lavage samples were purified using a NaBr discontinuous gradient. Concentrations of phospholipid classes and molecular species were measured using electrospray ionisation mass spectrometry, cholesterol was measured using high-performance liquid chromatography, surfactant protein A (SP-A) and SP-B were measured using enzyme-linked immunosorbent assays. There were small differences in phospholipid classes, with a lower level of anionic surfactant phospholipids, PG and PI, between diving and terrestrial mammals. There were no differences in PL saturation or SP-A levels between species. PC16:0/14:0, PC16:0/16:1, PC16:0/16:0, long chain PI species and the total concentrations of alkyl–acyl species of PC and PG as a ratio of diacyl species were increased in diving mammals, whereas concentrations of PC16:0/18:1, PG16:0/16:0 and PG16:0/18:1 were decreased. Cholesterol levels were very variable between species and SP-B was very low in diving mammals. These differences may explain the very poor surface activity of pinniped surfactant that we have previously described [Miller, N.J., Daniels, C.B., Schürch, S., Schoel, W.M., Orgeig, S., 2005. The surface activity of pulmonary surfactant from diving mammals. Article in Journal/Newspaper Elephant Seal ringed seal University of Southampton: e-Prints Soton |
institution |
Open Polar |
collection |
University of Southampton: e-Prints Soton |
op_collection_id |
ftsouthampton |
language |
unknown |
description |
Maintaining a functional pulmonary surfactant system at depth is critical for diving mammals to ensure that inspiration is possible upon re-emergence. The lipid and protein composition of lavage extracts from three pinniped species (California sea lion, Northern elephant seal and Ringed seal) were compared to several terrestrial species. Lavage samples were purified using a NaBr discontinuous gradient. Concentrations of phospholipid classes and molecular species were measured using electrospray ionisation mass spectrometry, cholesterol was measured using high-performance liquid chromatography, surfactant protein A (SP-A) and SP-B were measured using enzyme-linked immunosorbent assays. There were small differences in phospholipid classes, with a lower level of anionic surfactant phospholipids, PG and PI, between diving and terrestrial mammals. There were no differences in PL saturation or SP-A levels between species. PC16:0/14:0, PC16:0/16:1, PC16:0/16:0, long chain PI species and the total concentrations of alkyl–acyl species of PC and PG as a ratio of diacyl species were increased in diving mammals, whereas concentrations of PC16:0/18:1, PG16:0/16:0 and PG16:0/18:1 were decreased. Cholesterol levels were very variable between species and SP-B was very low in diving mammals. These differences may explain the very poor surface activity of pinniped surfactant that we have previously described [Miller, N.J., Daniels, C.B., Schürch, S., Schoel, W.M., Orgeig, S., 2005. The surface activity of pulmonary surfactant from diving mammals. |
format |
Article in Journal/Newspaper |
author |
Miller, Natalie J. Postle, Anthony D. Orgeig, Sandra Koster, Grielof Daniels, Christopher B. |
spellingShingle |
Miller, Natalie J. Postle, Anthony D. Orgeig, Sandra Koster, Grielof Daniels, Christopher B. The composition of pulmonary surfactant from diving mammals |
author_facet |
Miller, Natalie J. Postle, Anthony D. Orgeig, Sandra Koster, Grielof Daniels, Christopher B. |
author_sort |
Miller, Natalie J. |
title |
The composition of pulmonary surfactant from diving mammals |
title_short |
The composition of pulmonary surfactant from diving mammals |
title_full |
The composition of pulmonary surfactant from diving mammals |
title_fullStr |
The composition of pulmonary surfactant from diving mammals |
title_full_unstemmed |
The composition of pulmonary surfactant from diving mammals |
title_sort |
composition of pulmonary surfactant from diving mammals |
publishDate |
2006 |
url |
https://eprints.soton.ac.uk/27270/ |
genre |
Elephant Seal ringed seal |
genre_facet |
Elephant Seal ringed seal |
op_relation |
Miller, Natalie J., Postle, Anthony D., Orgeig, Sandra, Koster, Grielof and Daniels, Christopher B. (2006) The composition of pulmonary surfactant from diving mammals. Respiratory Physiology & Neurobiology, 152 (2), 152-168. (doi:10.1016/j.resp.2005.08.001 <http://dx.doi.org/10.1016/j.resp.2005.08.001>). |
_version_ |
1772814254575976448 |