Response of haemocyte lysosomes to bacterial inoculation in the oysters Ostrea edulis L. and Crassostrea gigas (Thunberg), and the scallop Pecten maximus (L).
Data are presented that demonstrate the application of the neutral red retention assay (NRR) to monitor the effcts of a bacterial inoculation on the haemocyte lysosomes of the European flat oyster Ostrea edulis, Pacific oyster Crassostrea gigas and scallop Pecten maximus. Bivalves were acclimated to...
Published in: | Fish & Shellfish Immunology |
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Language: | English |
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2000
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Online Access: | https://eprints.soton.ac.uk/24128/ https://eprints.soton.ac.uk/24128/1/24128-01.pdf |
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ftsouthampton:oai:eprints.soton.ac.uk:24128 2023-07-30T04:03:02+02:00 Response of haemocyte lysosomes to bacterial inoculation in the oysters Ostrea edulis L. and Crassostrea gigas (Thunberg), and the scallop Pecten maximus (L). Hauton, C. Hawkins, L.E. Hutchinson, S. 2000-06-07 text https://eprints.soton.ac.uk/24128/ https://eprints.soton.ac.uk/24128/1/24128-01.pdf en eng https://eprints.soton.ac.uk/24128/1/24128-01.pdf Hauton, C., Hawkins, L.E. and Hutchinson, S. (2000) Response of haemocyte lysosomes to bacterial inoculation in the oysters Ostrea edulis L. and Crassostrea gigas (Thunberg), and the scallop Pecten maximus (L). Fish and Shellfish Immunology, 11 (2), 143-153. (doi:10.1006/fsim.2000.0301 <http://dx.doi.org/10.1006/fsim.2000.0301>). (Submitted) Article PeerReviewed 2000 ftsouthampton https://doi.org/10.1006/fsim.2000.0301 2023-07-09T20:39:01Z Data are presented that demonstrate the application of the neutral red retention assay (NRR) to monitor the effcts of a bacterial inoculation on the haemocyte lysosomes of the European flat oyster Ostrea edulis, Pacific oyster Crassostrea gigas and scallop Pecten maximus. Bivalves were acclimated to three temperature regimes (5, 15 and 25 C), at constant salinity for 7 days in the laboratory. Once baseline responses to acclimation temperature had been established, the effects of an in vivo inoculation on haemocyte lysosomal stability were assessed using the NRR assay. Lysosomal membrane stability was reduced in the presence of bacteria for all three species of bivalve, but destabilisation of C. gigas haemocyte lysosomes appeared to be most sensitive to the presence of the bacterium Listonella anguillarum. For all three bivalve species, the reduction in lysosomal stability appeared to be proportional to the growth of the bacterial inoculate. Using appropriate controls, the NRR assay was demonstrated to have great potential as a tool with which to make rapid initial assessments of the immune status of bivalve molluscs. Article in Journal/Newspaper Crassostrea gigas Pacific oyster University of Southampton: e-Prints Soton Pacific Fish & Shellfish Immunology 11 2 143 153 |
institution |
Open Polar |
collection |
University of Southampton: e-Prints Soton |
op_collection_id |
ftsouthampton |
language |
English |
description |
Data are presented that demonstrate the application of the neutral red retention assay (NRR) to monitor the effcts of a bacterial inoculation on the haemocyte lysosomes of the European flat oyster Ostrea edulis, Pacific oyster Crassostrea gigas and scallop Pecten maximus. Bivalves were acclimated to three temperature regimes (5, 15 and 25 C), at constant salinity for 7 days in the laboratory. Once baseline responses to acclimation temperature had been established, the effects of an in vivo inoculation on haemocyte lysosomal stability were assessed using the NRR assay. Lysosomal membrane stability was reduced in the presence of bacteria for all three species of bivalve, but destabilisation of C. gigas haemocyte lysosomes appeared to be most sensitive to the presence of the bacterium Listonella anguillarum. For all three bivalve species, the reduction in lysosomal stability appeared to be proportional to the growth of the bacterial inoculate. Using appropriate controls, the NRR assay was demonstrated to have great potential as a tool with which to make rapid initial assessments of the immune status of bivalve molluscs. |
format |
Article in Journal/Newspaper |
author |
Hauton, C. Hawkins, L.E. Hutchinson, S. |
spellingShingle |
Hauton, C. Hawkins, L.E. Hutchinson, S. Response of haemocyte lysosomes to bacterial inoculation in the oysters Ostrea edulis L. and Crassostrea gigas (Thunberg), and the scallop Pecten maximus (L). |
author_facet |
Hauton, C. Hawkins, L.E. Hutchinson, S. |
author_sort |
Hauton, C. |
title |
Response of haemocyte lysosomes to bacterial inoculation in the oysters Ostrea edulis L. and Crassostrea gigas (Thunberg), and the scallop Pecten maximus (L). |
title_short |
Response of haemocyte lysosomes to bacterial inoculation in the oysters Ostrea edulis L. and Crassostrea gigas (Thunberg), and the scallop Pecten maximus (L). |
title_full |
Response of haemocyte lysosomes to bacterial inoculation in the oysters Ostrea edulis L. and Crassostrea gigas (Thunberg), and the scallop Pecten maximus (L). |
title_fullStr |
Response of haemocyte lysosomes to bacterial inoculation in the oysters Ostrea edulis L. and Crassostrea gigas (Thunberg), and the scallop Pecten maximus (L). |
title_full_unstemmed |
Response of haemocyte lysosomes to bacterial inoculation in the oysters Ostrea edulis L. and Crassostrea gigas (Thunberg), and the scallop Pecten maximus (L). |
title_sort |
response of haemocyte lysosomes to bacterial inoculation in the oysters ostrea edulis l. and crassostrea gigas (thunberg), and the scallop pecten maximus (l). |
publishDate |
2000 |
url |
https://eprints.soton.ac.uk/24128/ https://eprints.soton.ac.uk/24128/1/24128-01.pdf |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_relation |
https://eprints.soton.ac.uk/24128/1/24128-01.pdf Hauton, C., Hawkins, L.E. and Hutchinson, S. (2000) Response of haemocyte lysosomes to bacterial inoculation in the oysters Ostrea edulis L. and Crassostrea gigas (Thunberg), and the scallop Pecten maximus (L). Fish and Shellfish Immunology, 11 (2), 143-153. (doi:10.1006/fsim.2000.0301 <http://dx.doi.org/10.1006/fsim.2000.0301>). (Submitted) |
op_doi |
https://doi.org/10.1006/fsim.2000.0301 |
container_title |
Fish & Shellfish Immunology |
container_volume |
11 |
container_issue |
2 |
container_start_page |
143 |
op_container_end_page |
153 |
_version_ |
1772813964890079232 |