Real-time PCR quantification of the in vitro effects of crustacean immunostimulants on gene expression in lobster (Homarus gammarus) granular haemocytes.
This paper presents data from an investigation of the mode of action of five different crustacean immunostimulants presented to European lobster (Homarus gammarus) granulocytes cultured in vitro. The experiments were designed to test whether or not the immunostimulants could cause the short-term upr...
Published in: | Developmental & Comparative Immunology |
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Language: | English |
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2005
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Online Access: | https://eprints.soton.ac.uk/24112/ https://eprints.soton.ac.uk/24112/1/Hauton_et_al_2005_DCI.pdf |
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ftsouthampton:oai:eprints.soton.ac.uk:24112 2023-07-30T04:03:21+02:00 Real-time PCR quantification of the in vitro effects of crustacean immunostimulants on gene expression in lobster (Homarus gammarus) granular haemocytes. Hauton, C Hammond, J.A. Smith, V.J. 2005 text https://eprints.soton.ac.uk/24112/ https://eprints.soton.ac.uk/24112/1/Hauton_et_al_2005_DCI.pdf en eng https://eprints.soton.ac.uk/24112/1/Hauton_et_al_2005_DCI.pdf Hauton, C, Hammond, J.A. and Smith, V.J. (2005) Real-time PCR quantification of the in vitro effects of crustacean immunostimulants on gene expression in lobster (Homarus gammarus) granular haemocytes. Developmental and Comparative Immunology, 29 (1), 33-42. (doi:10.1016/j.dci.2004.05.006 <http://dx.doi.org/10.1016/j.dci.2004.05.006>). Article PeerReviewed 2005 ftsouthampton https://doi.org/10.1016/j.dci.2004.05.006 2023-07-09T20:39:01Z This paper presents data from an investigation of the mode of action of five different crustacean immunostimulants presented to European lobster (Homarus gammarus) granulocytes cultured in vitro. The experiments were designed to test whether or not the immunostimulants could cause the short-term upregulation of genes coding for immune proteins without causing the cells to degranulate. Quantitative measurements of mRNA transcript abundance were made using real-time PCR and it was first necessary to isolate the complete gene sequences coding for the proteins prophenoloxidase (proPO), ?-1,3-glucan binding protein (?GBP) and ?-actin ( ?-act) in the lobster. These sequences were used to design TaqMan™ primer and fluorescent probe sets. The presented data indicated that the majority of the tested immunostimulants did not induce the up-regulation of immune-related gene expression in the granulocytes in isolation. Alternative modes of action, including the in vivo up-regulation of gene expression in haemopoetic tissues, are discussed. Article in Journal/Newspaper European lobster Homarus gammarus University of Southampton: e-Prints Soton Developmental & Comparative Immunology 29 1 33 42 |
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Open Polar |
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University of Southampton: e-Prints Soton |
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ftsouthampton |
language |
English |
description |
This paper presents data from an investigation of the mode of action of five different crustacean immunostimulants presented to European lobster (Homarus gammarus) granulocytes cultured in vitro. The experiments were designed to test whether or not the immunostimulants could cause the short-term upregulation of genes coding for immune proteins without causing the cells to degranulate. Quantitative measurements of mRNA transcript abundance were made using real-time PCR and it was first necessary to isolate the complete gene sequences coding for the proteins prophenoloxidase (proPO), ?-1,3-glucan binding protein (?GBP) and ?-actin ( ?-act) in the lobster. These sequences were used to design TaqMan™ primer and fluorescent probe sets. The presented data indicated that the majority of the tested immunostimulants did not induce the up-regulation of immune-related gene expression in the granulocytes in isolation. Alternative modes of action, including the in vivo up-regulation of gene expression in haemopoetic tissues, are discussed. |
format |
Article in Journal/Newspaper |
author |
Hauton, C Hammond, J.A. Smith, V.J. |
spellingShingle |
Hauton, C Hammond, J.A. Smith, V.J. Real-time PCR quantification of the in vitro effects of crustacean immunostimulants on gene expression in lobster (Homarus gammarus) granular haemocytes. |
author_facet |
Hauton, C Hammond, J.A. Smith, V.J. |
author_sort |
Hauton, C |
title |
Real-time PCR quantification of the in vitro effects of crustacean immunostimulants on gene expression in lobster (Homarus gammarus) granular haemocytes. |
title_short |
Real-time PCR quantification of the in vitro effects of crustacean immunostimulants on gene expression in lobster (Homarus gammarus) granular haemocytes. |
title_full |
Real-time PCR quantification of the in vitro effects of crustacean immunostimulants on gene expression in lobster (Homarus gammarus) granular haemocytes. |
title_fullStr |
Real-time PCR quantification of the in vitro effects of crustacean immunostimulants on gene expression in lobster (Homarus gammarus) granular haemocytes. |
title_full_unstemmed |
Real-time PCR quantification of the in vitro effects of crustacean immunostimulants on gene expression in lobster (Homarus gammarus) granular haemocytes. |
title_sort |
real-time pcr quantification of the in vitro effects of crustacean immunostimulants on gene expression in lobster (homarus gammarus) granular haemocytes. |
publishDate |
2005 |
url |
https://eprints.soton.ac.uk/24112/ https://eprints.soton.ac.uk/24112/1/Hauton_et_al_2005_DCI.pdf |
genre |
European lobster Homarus gammarus |
genre_facet |
European lobster Homarus gammarus |
op_relation |
https://eprints.soton.ac.uk/24112/1/Hauton_et_al_2005_DCI.pdf Hauton, C, Hammond, J.A. and Smith, V.J. (2005) Real-time PCR quantification of the in vitro effects of crustacean immunostimulants on gene expression in lobster (Homarus gammarus) granular haemocytes. Developmental and Comparative Immunology, 29 (1), 33-42. (doi:10.1016/j.dci.2004.05.006 <http://dx.doi.org/10.1016/j.dci.2004.05.006>). |
op_doi |
https://doi.org/10.1016/j.dci.2004.05.006 |
container_title |
Developmental & Comparative Immunology |
container_volume |
29 |
container_issue |
1 |
container_start_page |
33 |
op_container_end_page |
42 |
_version_ |
1772814344819572736 |