Study of marine amoebae and their interactions with pathogenic vibrios for oyster
Free living amoebae inhabit aquatic environments and use phagocytosis of bacteria for their nutrition. According to the hypothesis of coincidental evolution of virulence, the cellular and molecular mechanisms of phagocytosis being preserved from amoebae to the immune cells of animals, the predation...
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Online Access: | https://theses.hal.science/tel-02481226 https://theses.hal.science/tel-02481226/document https://theses.hal.science/tel-02481226/file/2019_ROBINO_archivage.pdf |
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ftsorbonneuniv:oai:HAL:tel-02481226v1 2024-09-15T18:03:19+00:00 Study of marine amoebae and their interactions with pathogenic vibrios for oyster Etude des amibes marines et de leurs interactions avec les vibrios pathogènes d’huître Robino, Etienne Interactions Hôtes-Pathogènes-Environnements (IHPE) Université de Perpignan Via Domitia (UPVD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM) Université Montpellier Guillaume Charrière 2019-11-12 https://theses.hal.science/tel-02481226 https://theses.hal.science/tel-02481226/document https://theses.hal.science/tel-02481226/file/2019_ROBINO_archivage.pdf fr fre HAL CCSD NNT: 2019MONTG041 tel-02481226 https://theses.hal.science/tel-02481226 https://theses.hal.science/tel-02481226/document https://theses.hal.science/tel-02481226/file/2019_ROBINO_archivage.pdf info:eu-repo/semantics/OpenAccess https://theses.hal.science/tel-02481226 Sciences agricoles. Université Montpellier, 2019. Français. ⟨NNT : 2019MONTG041⟩ Free-Living amoebae Pathogenic vibrios Phagocytosis Coincidental evolution Diversity Amibes libres Vibrios pathogènes Phagocytose Évolution fortuite Diversité [SDV.SA]Life Sciences [q-bio]/Agricultural sciences info:eu-repo/semantics/doctoralThesis Theses 2019 ftsorbonneuniv 2024-07-25T23:48:00Z Free living amoebae inhabit aquatic environments and use phagocytosis of bacteria for their nutrition. According to the hypothesis of coincidental evolution of virulence, the cellular and molecular mechanisms of phagocytosis being preserved from amoebae to the immune cells of animals, the predation exerted by amoebae could favor the emergence of pathogenic bacteria resistant to phagocytosis. Since 2008, Crassostrea gigas oysters have suffered from over-mortality in France. This poly-microbial disease involves the Herpes OsHV-1 μvar virus which causes an immunosuppression of oysters that are then colonized by various opportunistic pathogenic bacteria including vibrios inducing the death of the animal. V. tasmaniensis LGP32 is a facultative intracellular pathogen of oyster hemocytes that resists phagocytosis and destroys hemocytes using different virulence factors. We have therefore undertaken to study the interactions between marine amoebae of the oyster environment and the vibrios in order to verify if some mechanisms of virulence could also play a role in this type of interactions. By performing field sampling, we demonstrated that the interaction between vibrios and amoebae is ecologically realistic and observed a low diversity of heterotrophic protists near the oyster tables of the Thau Lagoon compared to other less anthropogenic environments. Functional studies between LGP32 and the amoeba Vannella sp. AP1411 showed that LGP32 is able to resist amoeba predation involving certain virulence factors such as Vsm metalloprotease and CopA P-ATPase copper efflux pump which are also involved in the interaction of LGP32 with oysters. In contrast, other virulence factors implicated in the oyster are not involved in amoeba-predation resistance indicating that some factors are involved in interactions with various hosts while others would be involved in more specific interactions. Les amibes libres dans les environnements aquatiques utilisent la phagocytose des bactéries pour leur nutrition. Selon l’hypothèse de ... Doctoral or Postdoctoral Thesis Crassostrea gigas HAL Sorbonne Université |
institution |
Open Polar |
collection |
HAL Sorbonne Université |
op_collection_id |
ftsorbonneuniv |
language |
French |
topic |
Free-Living amoebae Pathogenic vibrios Phagocytosis Coincidental evolution Diversity Amibes libres Vibrios pathogènes Phagocytose Évolution fortuite Diversité [SDV.SA]Life Sciences [q-bio]/Agricultural sciences |
spellingShingle |
Free-Living amoebae Pathogenic vibrios Phagocytosis Coincidental evolution Diversity Amibes libres Vibrios pathogènes Phagocytose Évolution fortuite Diversité [SDV.SA]Life Sciences [q-bio]/Agricultural sciences Robino, Etienne Study of marine amoebae and their interactions with pathogenic vibrios for oyster |
topic_facet |
Free-Living amoebae Pathogenic vibrios Phagocytosis Coincidental evolution Diversity Amibes libres Vibrios pathogènes Phagocytose Évolution fortuite Diversité [SDV.SA]Life Sciences [q-bio]/Agricultural sciences |
description |
Free living amoebae inhabit aquatic environments and use phagocytosis of bacteria for their nutrition. According to the hypothesis of coincidental evolution of virulence, the cellular and molecular mechanisms of phagocytosis being preserved from amoebae to the immune cells of animals, the predation exerted by amoebae could favor the emergence of pathogenic bacteria resistant to phagocytosis. Since 2008, Crassostrea gigas oysters have suffered from over-mortality in France. This poly-microbial disease involves the Herpes OsHV-1 μvar virus which causes an immunosuppression of oysters that are then colonized by various opportunistic pathogenic bacteria including vibrios inducing the death of the animal. V. tasmaniensis LGP32 is a facultative intracellular pathogen of oyster hemocytes that resists phagocytosis and destroys hemocytes using different virulence factors. We have therefore undertaken to study the interactions between marine amoebae of the oyster environment and the vibrios in order to verify if some mechanisms of virulence could also play a role in this type of interactions. By performing field sampling, we demonstrated that the interaction between vibrios and amoebae is ecologically realistic and observed a low diversity of heterotrophic protists near the oyster tables of the Thau Lagoon compared to other less anthropogenic environments. Functional studies between LGP32 and the amoeba Vannella sp. AP1411 showed that LGP32 is able to resist amoeba predation involving certain virulence factors such as Vsm metalloprotease and CopA P-ATPase copper efflux pump which are also involved in the interaction of LGP32 with oysters. In contrast, other virulence factors implicated in the oyster are not involved in amoeba-predation resistance indicating that some factors are involved in interactions with various hosts while others would be involved in more specific interactions. Les amibes libres dans les environnements aquatiques utilisent la phagocytose des bactéries pour leur nutrition. Selon l’hypothèse de ... |
author2 |
Interactions Hôtes-Pathogènes-Environnements (IHPE) Université de Perpignan Via Domitia (UPVD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM) Université Montpellier Guillaume Charrière |
format |
Doctoral or Postdoctoral Thesis |
author |
Robino, Etienne |
author_facet |
Robino, Etienne |
author_sort |
Robino, Etienne |
title |
Study of marine amoebae and their interactions with pathogenic vibrios for oyster |
title_short |
Study of marine amoebae and their interactions with pathogenic vibrios for oyster |
title_full |
Study of marine amoebae and their interactions with pathogenic vibrios for oyster |
title_fullStr |
Study of marine amoebae and their interactions with pathogenic vibrios for oyster |
title_full_unstemmed |
Study of marine amoebae and their interactions with pathogenic vibrios for oyster |
title_sort |
study of marine amoebae and their interactions with pathogenic vibrios for oyster |
publisher |
HAL CCSD |
publishDate |
2019 |
url |
https://theses.hal.science/tel-02481226 https://theses.hal.science/tel-02481226/document https://theses.hal.science/tel-02481226/file/2019_ROBINO_archivage.pdf |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_source |
https://theses.hal.science/tel-02481226 Sciences agricoles. Université Montpellier, 2019. Français. ⟨NNT : 2019MONTG041⟩ |
op_relation |
NNT: 2019MONTG041 tel-02481226 https://theses.hal.science/tel-02481226 https://theses.hal.science/tel-02481226/document https://theses.hal.science/tel-02481226/file/2019_ROBINO_archivage.pdf |
op_rights |
info:eu-repo/semantics/OpenAccess |
_version_ |
1810440836388749312 |