Recombinant plant gamma carbonic anhydrase homotrimers bind inorganic carbon

• Published in: FEBS Letters
• Main Authors: Martin, Victoria, Villarreal, Fernando, Miras, Isabelle, Navaza, Alda, Haouz, Ahmed, González-Lebrero, Rodolfo, M., Kaufman, Sergio, B., Zabaleta, Eduardo
• Other Authors: Instituto de Investigaciones Biológicas Mar del Plata (IIB), Consejo Nacional de Investigaciones Científicas y Técnicas Buenos Aires (CONICET)-Facultad de Ciencias Exactas y Naturales Mar del Plata, Universidad Nacional de Mar del Plata Mar del Plata (UNMdP)-Universidad Nacional de Mar del Plata Mar del Plata (UNMdP), Cristallogenèse et Diffraction des Rayons X (Plate-forme/PF6), Institut Pasteur Paris (IP)-Centre National de la Recherche Scientifique (CNRS), Laboratoire Acides Nucléiques & Biophotonique (AnBiophi), Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut de Chimie - CNRS Chimie (INC-CNRS)-Centre National de la Recherche Scientifique (CNRS), Universidad de Buenos Aires Buenos Aires (UBA), This work was supported by Grants from the (ANPCyT, PICT 31669 and 00673), the Ministry of Science and Technology, Argentine-ECOS Sud France (MinCyT-ECOS A06B03) and the University of Mar del Plata, Argentine (OCS 341/08). V.M. and F.V. are doctoral fellows, S.K., R.G.L. and E.Z. are members of CONICET.
• Format: Article in Journal/Newspaper
• Language: English
• Published: HAL CCSD 2009
• Subjects: Gamma carbonic anhydrase; Carbon dioxide; Carbonate; Mitochondria; Arabidopsis thaliana; MESH: Amino Acid Sequence; MESH: Arabidopsis; MESH: Protein Structure; Quaternary; MESH: Protein Subunits; MESH: Recombinant Proteins; MESH: Solubility; MESH: Water; MESH: Bicarbonates; MESH: Carbon; MESH: Carbon Radioisotopes; MESH: Carbonic Acid; MESH: Carbonic Anhydrase II; MESH: Molecular Sequence Data; MESH: Protein Binding; MESH: Protein Multimerization; [SDV]Life Sciences [q-bio]; Carbonic acid
• Online Access: https://pasteur.hal.science/pasteur-03525339; https://doi.org/10.1016/j.febslet.2009.09.055

International audience Gamma carbonic anhydrases (cCA) are widespread in Prokaryotes. In Eukaryotes, homologous genes were found only in plant genomes. In Arabidopsis and maize, the corresponding gene products are subunits of mitochondrial Complex I. At present, only cCA homotrimers of Methanosarcina thermophila (CAM) show reversible carbon dioxide (CO 2) hydration activity. In the present work, it is shown that recombinant plant cCA2 could form homotrimers and bind H 14 CO À 3. However, they are unable to catalyse the reversible hydration of CO 2. These results suggest that plant cCAs do not act as carbonic anhydrases but with a related activity possibly contributing to recycle CO 2 in the context of photorespiration.