Culturable diversity of Arctic phytoplankton during pack ice melting
Abstract Massive phytoplankton blooms develop at the Arctic ice edge, sometimes extending far under the pack ice. An extensive culturing effort was conducted before and during a phytoplankton bloom in Baffin Bay between April and July 2016. Different isolation strategies were applied, including flow...
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ftsorbonneuniv:oai:HAL:hal-03971904v1 2023-05-15T14:57:49+02:00 Culturable diversity of Arctic phytoplankton during pack ice melting Ribeiro, Catherine Gérikas dos Santos, Adriana Lopes Gourvil, Priscillia Gall, Florence Le Marie, Dominique Tragin, Margot Probert, Ian Vaulot, Daniel Adaptation et diversité en milieu marin (ADMM) Institut national des sciences de l'Univers (INSU - CNRS)-Station biologique de Roscoff (SBR) Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS) Sorbonne Université (SU) Station biologique de Roscoff (SBR) Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS) 2023-02-03 https://hal.science/hal-03971904 https://doi.org/10.1101/642264 en eng HAL CCSD info:eu-repo/semantics/altIdentifier/doi/10.1101/642264 hal-03971904 https://hal.science/hal-03971904 BIORXIV: 642264 doi:10.1101/642264 https://hal.science/hal-03971904 2023 [SDE]Environmental Sciences info:eu-repo/semantics/preprint Preprints, Working Papers, . 2023 ftsorbonneuniv https://doi.org/10.1101/642264 2023-02-07T23:43:07Z Abstract Massive phytoplankton blooms develop at the Arctic ice edge, sometimes extending far under the pack ice. An extensive culturing effort was conducted before and during a phytoplankton bloom in Baffin Bay between April and July 2016. Different isolation strategies were applied, including flow cytometry cell sorting, manual single cell pipetting and serial dilution. Although all three techniques yielded the most common organisms, each technique retrieved specific taxa, highlighting the importance of using several methods to maximize the number and diversity of isolated strains. More than 1,000 cultures were obtained, characterized by 18S rRNA sequencing and optical microscopy and de-replicated to a subset of 276 strains presented in this work. Strains grouped into 57 genotypes defined by 100% 18S rRNA sequence similarity. These genotypes spread across five divisions: Heterokontophyta, Chlorophyta, Cryptophyta, Haptophyta and Dinophyta. Diatoms were the most abundant group (193 strains), mostly represented by the genera Chaetoceros and Attheya . The genera Rhodomonas and Pyramimonas were the most abundant non-diatom nanoplankton strains, while Micromonas polaris dominated the picoplankton. Diversity at the class level was higher during the peak of the bloom. Potentially new species were isolated, in particular within the genera Navicula , Nitzschia , Coscinodiscus , Thalassiosira , Pyramimonas , Mantoniella and Isochrysis . Submitted to: Elementa: Science of the Anthropocene Date: May 17, 2019 Report Arctic Baffin Bay Baffin Bay Baffin Phytoplankton HAL Sorbonne Université Arctic Baffin Bay |
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English |
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[SDE]Environmental Sciences |
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[SDE]Environmental Sciences Ribeiro, Catherine Gérikas dos Santos, Adriana Lopes Gourvil, Priscillia Gall, Florence Le Marie, Dominique Tragin, Margot Probert, Ian Vaulot, Daniel Culturable diversity of Arctic phytoplankton during pack ice melting |
topic_facet |
[SDE]Environmental Sciences |
description |
Abstract Massive phytoplankton blooms develop at the Arctic ice edge, sometimes extending far under the pack ice. An extensive culturing effort was conducted before and during a phytoplankton bloom in Baffin Bay between April and July 2016. Different isolation strategies were applied, including flow cytometry cell sorting, manual single cell pipetting and serial dilution. Although all three techniques yielded the most common organisms, each technique retrieved specific taxa, highlighting the importance of using several methods to maximize the number and diversity of isolated strains. More than 1,000 cultures were obtained, characterized by 18S rRNA sequencing and optical microscopy and de-replicated to a subset of 276 strains presented in this work. Strains grouped into 57 genotypes defined by 100% 18S rRNA sequence similarity. These genotypes spread across five divisions: Heterokontophyta, Chlorophyta, Cryptophyta, Haptophyta and Dinophyta. Diatoms were the most abundant group (193 strains), mostly represented by the genera Chaetoceros and Attheya . The genera Rhodomonas and Pyramimonas were the most abundant non-diatom nanoplankton strains, while Micromonas polaris dominated the picoplankton. Diversity at the class level was higher during the peak of the bloom. Potentially new species were isolated, in particular within the genera Navicula , Nitzschia , Coscinodiscus , Thalassiosira , Pyramimonas , Mantoniella and Isochrysis . Submitted to: Elementa: Science of the Anthropocene Date: May 17, 2019 |
author2 |
Adaptation et diversité en milieu marin (ADMM) Institut national des sciences de l'Univers (INSU - CNRS)-Station biologique de Roscoff (SBR) Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS) Sorbonne Université (SU) Station biologique de Roscoff (SBR) Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS) |
format |
Report |
author |
Ribeiro, Catherine Gérikas dos Santos, Adriana Lopes Gourvil, Priscillia Gall, Florence Le Marie, Dominique Tragin, Margot Probert, Ian Vaulot, Daniel |
author_facet |
Ribeiro, Catherine Gérikas dos Santos, Adriana Lopes Gourvil, Priscillia Gall, Florence Le Marie, Dominique Tragin, Margot Probert, Ian Vaulot, Daniel |
author_sort |
Ribeiro, Catherine Gérikas |
title |
Culturable diversity of Arctic phytoplankton during pack ice melting |
title_short |
Culturable diversity of Arctic phytoplankton during pack ice melting |
title_full |
Culturable diversity of Arctic phytoplankton during pack ice melting |
title_fullStr |
Culturable diversity of Arctic phytoplankton during pack ice melting |
title_full_unstemmed |
Culturable diversity of Arctic phytoplankton during pack ice melting |
title_sort |
culturable diversity of arctic phytoplankton during pack ice melting |
publisher |
HAL CCSD |
publishDate |
2023 |
url |
https://hal.science/hal-03971904 https://doi.org/10.1101/642264 |
geographic |
Arctic Baffin Bay |
geographic_facet |
Arctic Baffin Bay |
genre |
Arctic Baffin Bay Baffin Bay Baffin Phytoplankton |
genre_facet |
Arctic Baffin Bay Baffin Bay Baffin Phytoplankton |
op_source |
https://hal.science/hal-03971904 2023 |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1101/642264 hal-03971904 https://hal.science/hal-03971904 BIORXIV: 642264 doi:10.1101/642264 |
op_doi |
https://doi.org/10.1101/642264 |
_version_ |
1766329926379634688 |