Diversity and molecular epidemiology of Ostreid herpesvirus 1 in farmed Crassostrea gigas in Australia: Geographic clusters and implications for “microvariants” in global mortality events
International audience Since 2010, mass mortality events known as Pacific oyster mortality syndrome (POMS) have occurred in Crassostrea gigas in Australia associated with Ostreid herpesvirus 1. The virus was thought to be an OsHV-1 µVar or "microvariant", i.e. one of the dominant variants...
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Online Access: | https://hal-normandie-univ.archives-ouvertes.fr/hal-03842646 https://doi.org/10.1016/j.virusres.2022.198994 |
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ftsorbonneuniv:oai:HAL:hal-03842646v1 2023-06-11T04:11:04+02:00 Diversity and molecular epidemiology of Ostreid herpesvirus 1 in farmed Crassostrea gigas in Australia: Geographic clusters and implications for “microvariants” in global mortality events Trancart, Suzanne Tweedie, Alison Liu, Olivia Paul-Pont, Ika Hick, Paul Houssin, Maryline Whittington, Richard LABÉO, Pôle d’analyses et de recherche de Normandie (LABÉO) Sydney School of Veterinary Science Faculty of Science The University of Sydney -The University of Sydney The University of Sydney Biologie des Organismes et Ecosystèmes Aquatiques (BOREA) Université de Caen Normandie (UNICAEN) Normandie Université (NU)-Normandie Université (NU)-Muséum national d'Histoire naturelle (MNHN)-Institut de Recherche pour le Développement (IRD)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Université des Antilles (UA) The University of Sidney 2023-01 https://hal-normandie-univ.archives-ouvertes.fr/hal-03842646 https://doi.org/10.1016/j.virusres.2022.198994 en eng HAL CCSD Elsevier info:eu-repo/semantics/altIdentifier/doi/10.1016/j.virusres.2022.198994 hal-03842646 https://hal-normandie-univ.archives-ouvertes.fr/hal-03842646 doi:10.1016/j.virusres.2022.198994 ISSN: 0168-1702 Virus Research https://hal-normandie-univ.archives-ouvertes.fr/hal-03842646 Virus Research, 2023, 323, pp.198994. ⟨10.1016/j.virusres.2022.198994⟩ Australia Crassostrea gigas Ostreid herpesvirus diversity phylogeny polymorphism variant [SDV.BA.MVSA]Life Sciences [q-bio]/Animal biology/Veterinary medicine and animal Health info:eu-repo/semantics/article Journal articles 2023 ftsorbonneuniv https://doi.org/10.1016/j.virusres.2022.198994 2023-05-29T17:58:12Z International audience Since 2010, mass mortality events known as Pacific oyster mortality syndrome (POMS) have occurred in Crassostrea gigas in Australia associated with Ostreid herpesvirus 1. The virus was thought to be an OsHV-1 µVar or "microvariant", i.e. one of the dominant variants associated with POMS in Europe, but there are few data to characterize the genotype in Australia. Consequently, the genetic identity and diversity of the virus was determined to understand the epidemiology of the disease in Australia. Samples were analysed from diseased C. gigas over five summer seasons between 2011 and 2016 in POMS-affected estuaries: Georges River in New South Wales (NSW), Hawkesbury River (NSW) and Pitt Water in Tasmania. Sequencing was attempted for six genomic regions. Numerous variants were identified among these regions (n = 100 isolates) while twelve variants were identified from concatenated nucleotide sequences (n = 61 isolates). Nucleotide diversity of the seven genotypes of C region among Australian isolates (Pi 0.99 × 10-3) was the lowest globally. All Australian isolates grouped in a cluster distinct from other OsHV-1 isolates worldwide. This is the first report that Australian outbreaks of POMS were associated with OsHV-1 distinct from OsHV-1 reference genotype, µVar and other microvariants from other countries. The findings illustrate that microvariants are not the only variants of OsHV-1 associated with mass mortality events in C. gigas. In addition, there was mutually exclusive spatial clustering of viral genomic and amino acid sequence variants within estuaries, and a possible association between genotype/amino acid sequence and the prevalence and severity of POMS, as this differed between these estuaries. The sequencing findings supported prior epidemiological evidence for environmental reservoirs of OsHV-1 for POMS outbreaks in Australia. Article in Journal/Newspaper Crassostrea gigas Pacific oyster HAL Sorbonne Université Pacific Virus Research 323 198994 |
institution |
Open Polar |
collection |
HAL Sorbonne Université |
op_collection_id |
ftsorbonneuniv |
language |
English |
topic |
Australia Crassostrea gigas Ostreid herpesvirus diversity phylogeny polymorphism variant [SDV.BA.MVSA]Life Sciences [q-bio]/Animal biology/Veterinary medicine and animal Health |
spellingShingle |
Australia Crassostrea gigas Ostreid herpesvirus diversity phylogeny polymorphism variant [SDV.BA.MVSA]Life Sciences [q-bio]/Animal biology/Veterinary medicine and animal Health Trancart, Suzanne Tweedie, Alison Liu, Olivia Paul-Pont, Ika Hick, Paul Houssin, Maryline Whittington, Richard Diversity and molecular epidemiology of Ostreid herpesvirus 1 in farmed Crassostrea gigas in Australia: Geographic clusters and implications for “microvariants” in global mortality events |
topic_facet |
Australia Crassostrea gigas Ostreid herpesvirus diversity phylogeny polymorphism variant [SDV.BA.MVSA]Life Sciences [q-bio]/Animal biology/Veterinary medicine and animal Health |
description |
International audience Since 2010, mass mortality events known as Pacific oyster mortality syndrome (POMS) have occurred in Crassostrea gigas in Australia associated with Ostreid herpesvirus 1. The virus was thought to be an OsHV-1 µVar or "microvariant", i.e. one of the dominant variants associated with POMS in Europe, but there are few data to characterize the genotype in Australia. Consequently, the genetic identity and diversity of the virus was determined to understand the epidemiology of the disease in Australia. Samples were analysed from diseased C. gigas over five summer seasons between 2011 and 2016 in POMS-affected estuaries: Georges River in New South Wales (NSW), Hawkesbury River (NSW) and Pitt Water in Tasmania. Sequencing was attempted for six genomic regions. Numerous variants were identified among these regions (n = 100 isolates) while twelve variants were identified from concatenated nucleotide sequences (n = 61 isolates). Nucleotide diversity of the seven genotypes of C region among Australian isolates (Pi 0.99 × 10-3) was the lowest globally. All Australian isolates grouped in a cluster distinct from other OsHV-1 isolates worldwide. This is the first report that Australian outbreaks of POMS were associated with OsHV-1 distinct from OsHV-1 reference genotype, µVar and other microvariants from other countries. The findings illustrate that microvariants are not the only variants of OsHV-1 associated with mass mortality events in C. gigas. In addition, there was mutually exclusive spatial clustering of viral genomic and amino acid sequence variants within estuaries, and a possible association between genotype/amino acid sequence and the prevalence and severity of POMS, as this differed between these estuaries. The sequencing findings supported prior epidemiological evidence for environmental reservoirs of OsHV-1 for POMS outbreaks in Australia. |
author2 |
LABÉO, Pôle d’analyses et de recherche de Normandie (LABÉO) Sydney School of Veterinary Science Faculty of Science The University of Sydney -The University of Sydney The University of Sydney Biologie des Organismes et Ecosystèmes Aquatiques (BOREA) Université de Caen Normandie (UNICAEN) Normandie Université (NU)-Normandie Université (NU)-Muséum national d'Histoire naturelle (MNHN)-Institut de Recherche pour le Développement (IRD)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Université des Antilles (UA) The University of Sidney |
format |
Article in Journal/Newspaper |
author |
Trancart, Suzanne Tweedie, Alison Liu, Olivia Paul-Pont, Ika Hick, Paul Houssin, Maryline Whittington, Richard |
author_facet |
Trancart, Suzanne Tweedie, Alison Liu, Olivia Paul-Pont, Ika Hick, Paul Houssin, Maryline Whittington, Richard |
author_sort |
Trancart, Suzanne |
title |
Diversity and molecular epidemiology of Ostreid herpesvirus 1 in farmed Crassostrea gigas in Australia: Geographic clusters and implications for “microvariants” in global mortality events |
title_short |
Diversity and molecular epidemiology of Ostreid herpesvirus 1 in farmed Crassostrea gigas in Australia: Geographic clusters and implications for “microvariants” in global mortality events |
title_full |
Diversity and molecular epidemiology of Ostreid herpesvirus 1 in farmed Crassostrea gigas in Australia: Geographic clusters and implications for “microvariants” in global mortality events |
title_fullStr |
Diversity and molecular epidemiology of Ostreid herpesvirus 1 in farmed Crassostrea gigas in Australia: Geographic clusters and implications for “microvariants” in global mortality events |
title_full_unstemmed |
Diversity and molecular epidemiology of Ostreid herpesvirus 1 in farmed Crassostrea gigas in Australia: Geographic clusters and implications for “microvariants” in global mortality events |
title_sort |
diversity and molecular epidemiology of ostreid herpesvirus 1 in farmed crassostrea gigas in australia: geographic clusters and implications for “microvariants” in global mortality events |
publisher |
HAL CCSD |
publishDate |
2023 |
url |
https://hal-normandie-univ.archives-ouvertes.fr/hal-03842646 https://doi.org/10.1016/j.virusres.2022.198994 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
ISSN: 0168-1702 Virus Research https://hal-normandie-univ.archives-ouvertes.fr/hal-03842646 Virus Research, 2023, 323, pp.198994. ⟨10.1016/j.virusres.2022.198994⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.virusres.2022.198994 hal-03842646 https://hal-normandie-univ.archives-ouvertes.fr/hal-03842646 doi:10.1016/j.virusres.2022.198994 |
op_doi |
https://doi.org/10.1016/j.virusres.2022.198994 |
container_title |
Virus Research |
container_volume |
323 |
container_start_page |
198994 |
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