Community composition of bacteria associated with cold-water coral Madrepora oculata: within and between colony variability
International audience Cold-water coral ecosystems are more widespread, diverse and productive than previously thought. However, little is known about the interaction of deep-water corals with microorganisms. To understand whether coral species have specific prokaryotic communities, it is necessary...
Published in: | Marine Ecology Progress Series |
---|---|
Main Authors: | , , , |
Other Authors: | , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
HAL CCSD
2009
|
Subjects: | |
Online Access: | https://hal.science/hal-03505036 https://hal.science/hal-03505036/document https://hal.science/hal-03505036/file/m397p089.pdf https://doi.org/10.3354/meps08429 |
id |
ftsorbonneuniv:oai:HAL:hal-03505036v1 |
---|---|
record_format |
openpolar |
institution |
Open Polar |
collection |
HAL Sorbonne Université |
op_collection_id |
ftsorbonneuniv |
language |
English |
topic |
[SDU.STU.OC]Sciences of the Universe [physics]/Earth Sciences/Oceanography |
spellingShingle |
[SDU.STU.OC]Sciences of the Universe [physics]/Earth Sciences/Oceanography Hansson, Lina Agis, Martin Maier, Cornelia Weinbauer, Markus, G Community composition of bacteria associated with cold-water coral Madrepora oculata: within and between colony variability |
topic_facet |
[SDU.STU.OC]Sciences of the Universe [physics]/Earth Sciences/Oceanography |
description |
International audience Cold-water coral ecosystems are more widespread, diverse and productive than previously thought. However, little is known about the interaction of deep-water corals with microorganisms. To understand whether coral species have specific prokaryotic communities, it is necessary to assess the within and between colony variability. This was studied based on 16S rRNA gene and denaturing gradient gel electrophoresis (DGGE) for one of the main cold-water corals Madrepora oculata at Rockall Bank off the coast of Ireland. We successfully applied a rapid, non-toxic and inexpensive method for extracting DNA for 16S rRNA gene fingerprinting of marine prokaryotic communities based on a heat and salt lysis with simultaneous salt extraction (HEATSALT). The within and between colony variability of the community composition of bacteria associated to the mucus and ectodermal tissue of M. oculata was then evaluated using a 16S rRNA gene PCR and DGGE approach. Bacterial community composition (BCC) clearly differed between living coral and reference samples (dead coral and surrounding water; 80% dissimilarity). A large within (35-40% dissimilarity between polyps) and between colony variability (ca. 50% dissimilarity) of BCC was detected. We also found preliminary evidence that BCC differed between M. oculata and Lophelia pertusa. The high intraspecific variability found has consequences for selecting sampling strategies when assessing bacterial diversity and refines the question of controlling mechanisms of bacterial diversity on corals. Sequencing of DGGE bands showed that Spongiobacter type phylotypes (STP) dominated the DGGE bands. STP of M, oculata were grouped together and were different from those detected in other corals and sponges. In addition, the high sequence diversity of STP Suggests specific ecological roles and adaptations of this group in M oculata. |
author2 |
Ocean Acidification International Coordination Centre, International Atomic Energy Agency Environment Laboratories Ecologie des systèmes marins côtiers (Ecosym) Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Centre National de la Recherche Scientifique (CNRS) Observatoire océanologique de Villefranche-sur-mer (OOVM) Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS) Laboratoire d'océanographie de Villefranche (LOV) Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Institut national des sciences de l'Univers (INSU - CNRS)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Institut de la Mer de Villefranche (IMEV) Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS) |
format |
Article in Journal/Newspaper |
author |
Hansson, Lina Agis, Martin Maier, Cornelia Weinbauer, Markus, G |
author_facet |
Hansson, Lina Agis, Martin Maier, Cornelia Weinbauer, Markus, G |
author_sort |
Hansson, Lina |
title |
Community composition of bacteria associated with cold-water coral Madrepora oculata: within and between colony variability |
title_short |
Community composition of bacteria associated with cold-water coral Madrepora oculata: within and between colony variability |
title_full |
Community composition of bacteria associated with cold-water coral Madrepora oculata: within and between colony variability |
title_fullStr |
Community composition of bacteria associated with cold-water coral Madrepora oculata: within and between colony variability |
title_full_unstemmed |
Community composition of bacteria associated with cold-water coral Madrepora oculata: within and between colony variability |
title_sort |
community composition of bacteria associated with cold-water coral madrepora oculata: within and between colony variability |
publisher |
HAL CCSD |
publishDate |
2009 |
url |
https://hal.science/hal-03505036 https://hal.science/hal-03505036/document https://hal.science/hal-03505036/file/m397p089.pdf https://doi.org/10.3354/meps08429 |
genre |
Lophelia pertusa |
genre_facet |
Lophelia pertusa |
op_source |
ISSN: 0171-8630 EISSN: 1616-1599 Marine Ecology Progress Series https://hal.science/hal-03505036 Marine Ecology Progress Series, 2009, 397, pp.89-102. ⟨10.3354/meps08429⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.3354/meps08429 hal-03505036 https://hal.science/hal-03505036 https://hal.science/hal-03505036/document https://hal.science/hal-03505036/file/m397p089.pdf doi:10.3354/meps08429 |
op_rights |
http://creativecommons.org/licenses/by/ info:eu-repo/semantics/OpenAccess |
op_doi |
https://doi.org/10.3354/meps08429 |
container_title |
Marine Ecology Progress Series |
container_volume |
397 |
container_start_page |
89 |
op_container_end_page |
102 |
_version_ |
1810456195449749504 |
spelling |
ftsorbonneuniv:oai:HAL:hal-03505036v1 2024-09-15T18:18:03+00:00 Community composition of bacteria associated with cold-water coral Madrepora oculata: within and between colony variability Hansson, Lina Agis, Martin Maier, Cornelia Weinbauer, Markus, G Ocean Acidification International Coordination Centre, International Atomic Energy Agency Environment Laboratories Ecologie des systèmes marins côtiers (Ecosym) Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Centre National de la Recherche Scientifique (CNRS) Observatoire océanologique de Villefranche-sur-mer (OOVM) Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS) Laboratoire d'océanographie de Villefranche (LOV) Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Institut national des sciences de l'Univers (INSU - CNRS)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Institut de la Mer de Villefranche (IMEV) Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS) 2009 https://hal.science/hal-03505036 https://hal.science/hal-03505036/document https://hal.science/hal-03505036/file/m397p089.pdf https://doi.org/10.3354/meps08429 en eng HAL CCSD Inter Research info:eu-repo/semantics/altIdentifier/doi/10.3354/meps08429 hal-03505036 https://hal.science/hal-03505036 https://hal.science/hal-03505036/document https://hal.science/hal-03505036/file/m397p089.pdf doi:10.3354/meps08429 http://creativecommons.org/licenses/by/ info:eu-repo/semantics/OpenAccess ISSN: 0171-8630 EISSN: 1616-1599 Marine Ecology Progress Series https://hal.science/hal-03505036 Marine Ecology Progress Series, 2009, 397, pp.89-102. ⟨10.3354/meps08429⟩ [SDU.STU.OC]Sciences of the Universe [physics]/Earth Sciences/Oceanography info:eu-repo/semantics/article Journal articles 2009 ftsorbonneuniv https://doi.org/10.3354/meps08429 2024-07-25T23:47:52Z International audience Cold-water coral ecosystems are more widespread, diverse and productive than previously thought. However, little is known about the interaction of deep-water corals with microorganisms. To understand whether coral species have specific prokaryotic communities, it is necessary to assess the within and between colony variability. This was studied based on 16S rRNA gene and denaturing gradient gel electrophoresis (DGGE) for one of the main cold-water corals Madrepora oculata at Rockall Bank off the coast of Ireland. We successfully applied a rapid, non-toxic and inexpensive method for extracting DNA for 16S rRNA gene fingerprinting of marine prokaryotic communities based on a heat and salt lysis with simultaneous salt extraction (HEATSALT). The within and between colony variability of the community composition of bacteria associated to the mucus and ectodermal tissue of M. oculata was then evaluated using a 16S rRNA gene PCR and DGGE approach. Bacterial community composition (BCC) clearly differed between living coral and reference samples (dead coral and surrounding water; 80% dissimilarity). A large within (35-40% dissimilarity between polyps) and between colony variability (ca. 50% dissimilarity) of BCC was detected. We also found preliminary evidence that BCC differed between M. oculata and Lophelia pertusa. The high intraspecific variability found has consequences for selecting sampling strategies when assessing bacterial diversity and refines the question of controlling mechanisms of bacterial diversity on corals. Sequencing of DGGE bands showed that Spongiobacter type phylotypes (STP) dominated the DGGE bands. STP of M, oculata were grouped together and were different from those detected in other corals and sponges. In addition, the high sequence diversity of STP Suggests specific ecological roles and adaptations of this group in M oculata. Article in Journal/Newspaper Lophelia pertusa HAL Sorbonne Université Marine Ecology Progress Series 397 89 102 |