Identification and stable expression of vitellogenin receptor through vitellogenesis in the European eel
International audience In teleosts, vitellogenin (Vtg) is a phospholipoglycoprotein synthesized by the liver, released into the blood circulation and incorporated into the oocytes via endocytosis mediated by the Vtg receptor (VTGR) to form the yolk granules. The VTGR is crucial for oocyte growth in...
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ftsorbonneuniv:oai:HAL:hal-02989391v1 2024-09-15T17:39:39+00:00 Identification and stable expression of vitellogenin receptor through vitellogenesis in the European eel Morini, M. Lafont, Anne-Gaelle Maugars, G. Baloche, S. Dufour, S. Asturiano, J. Perez, L. Centre de Ressources Biologiques APHP-SU (PASS-CRB-APHP-SU) Unité Mixte de Service Production et Analyse de données en Sciences de la vie et en Santé (PASS) Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU) Biologie des Organismes et Ecosystèmes Aquatiques (BOREA) Université de Caen Normandie (UNICAEN) Normandie Université (NU)-Normandie Université (NU)-Muséum national d'Histoire naturelle (MNHN)-Institut de Recherche pour le Développement (IRD)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Université des Antilles (UA) 2020-06 https://hal.science/hal-02989391 https://doi.org/10.1017/S1751731119003355 en eng HAL CCSD Published by Elsevier (since 2021) / Cambridge University Press (until 2020) info:eu-repo/semantics/altIdentifier/doi/10.1017/S1751731119003355 info:eu-repo/semantics/altIdentifier/pmid/31971122 hal-02989391 https://hal.science/hal-02989391 doi:10.1017/S1751731119003355 PUBMED: 31971122 ISSN: 1751-7311 EISSN: 1751-732X Animal https://hal.science/hal-02989391 Animal, 2020, 14 (6), pp.1213-1222. ⟨10.1017/S1751731119003355⟩ Anguilla anguilla lipoprotein receptor 8 oogenesis reproduction teleost MESH: Anguilla MESH: Animals MESH: Receptors LDL MESH: Sexual Maturation MESH: Vitellogenesis MESH: Vitellogenins MESH: Egg Proteins MESH: Female MESH: Gene Expression Regulation MESH: Liver MESH: Oocytes MESH: Ovary MESH: Pituitary Gland Cell Surface [SDV]Life Sciences [q-bio] info:eu-repo/semantics/article Journal articles 2020 ftsorbonneuniv https://doi.org/10.1017/S1751731119003355 2024-07-25T23:47:57Z International audience In teleosts, vitellogenin (Vtg) is a phospholipoglycoprotein synthesized by the liver, released into the blood circulation and incorporated into the oocytes via endocytosis mediated by the Vtg receptor (VTGR) to form the yolk granules. The VTGR is crucial for oocyte growth in egg-laying animals but is also present in non-oviparous vertebrates, such as human. The VTGR belongs to the low-density lipoprotein receptor superfamily (LDLR) and is also named very-low-density lipoprotein receptor (VLDLR). In this study, we identified and phylogenetically positioned the VTGR of a basal teleost, the European eel, Anguilla anguilla. We developed quantitative real-time PCR (qRT-PCR) and investigated the tissue distribution of vtgr transcripts. We compared by qRT-PCR the ovarian expression levels of vtgr in juvenile yellow eels and pre-pubertal silver eels. We also analyzed the regulation of ovarian vtgr expression throughout vitellogenesis in experimentally matured eels. The Vtg plasma level was measured by homologous ELISA experimental maturation. Our in silico search and phylogenetical analysis revealed a single vtgr in the European eel, orthologous to other vertebrate vtgr. The qRT-PCR studies revealed that vtgr is mainly expressed in the ovary and also detected in various other tissues such as brain, pituitary, gill, fat, heart, and testis, suggesting some extra-ovarian functions of VTGR. We showed that vtgr is expressed in ovaries of juvenile yellow eels with no higher expression in pre-pubertal silver eels nor in experimentally matured eels. This suggests that vtgr transcription already occurs during early pre-vitellogenesis of immature eels and is not further activated in vitellogenic oocytes. European eel Vtg plasma level increased throughout experimental maturation in agreement with previous studies. Taken together, these results suggest that vtgr transcript levels may not be a limiting step for the uptake of Vtg by the oocyte in the European eel. Article in Journal/Newspaper Anguilla anguilla HAL Sorbonne Université Animal 14 6 1213 1222 |
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Open Polar |
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HAL Sorbonne Université |
op_collection_id |
ftsorbonneuniv |
language |
English |
topic |
Anguilla anguilla lipoprotein receptor 8 oogenesis reproduction teleost MESH: Anguilla MESH: Animals MESH: Receptors LDL MESH: Sexual Maturation MESH: Vitellogenesis MESH: Vitellogenins MESH: Egg Proteins MESH: Female MESH: Gene Expression Regulation MESH: Liver MESH: Oocytes MESH: Ovary MESH: Pituitary Gland Cell Surface [SDV]Life Sciences [q-bio] |
spellingShingle |
Anguilla anguilla lipoprotein receptor 8 oogenesis reproduction teleost MESH: Anguilla MESH: Animals MESH: Receptors LDL MESH: Sexual Maturation MESH: Vitellogenesis MESH: Vitellogenins MESH: Egg Proteins MESH: Female MESH: Gene Expression Regulation MESH: Liver MESH: Oocytes MESH: Ovary MESH: Pituitary Gland Cell Surface [SDV]Life Sciences [q-bio] Morini, M. Lafont, Anne-Gaelle Maugars, G. Baloche, S. Dufour, S. Asturiano, J. Perez, L. Identification and stable expression of vitellogenin receptor through vitellogenesis in the European eel |
topic_facet |
Anguilla anguilla lipoprotein receptor 8 oogenesis reproduction teleost MESH: Anguilla MESH: Animals MESH: Receptors LDL MESH: Sexual Maturation MESH: Vitellogenesis MESH: Vitellogenins MESH: Egg Proteins MESH: Female MESH: Gene Expression Regulation MESH: Liver MESH: Oocytes MESH: Ovary MESH: Pituitary Gland Cell Surface [SDV]Life Sciences [q-bio] |
description |
International audience In teleosts, vitellogenin (Vtg) is a phospholipoglycoprotein synthesized by the liver, released into the blood circulation and incorporated into the oocytes via endocytosis mediated by the Vtg receptor (VTGR) to form the yolk granules. The VTGR is crucial for oocyte growth in egg-laying animals but is also present in non-oviparous vertebrates, such as human. The VTGR belongs to the low-density lipoprotein receptor superfamily (LDLR) and is also named very-low-density lipoprotein receptor (VLDLR). In this study, we identified and phylogenetically positioned the VTGR of a basal teleost, the European eel, Anguilla anguilla. We developed quantitative real-time PCR (qRT-PCR) and investigated the tissue distribution of vtgr transcripts. We compared by qRT-PCR the ovarian expression levels of vtgr in juvenile yellow eels and pre-pubertal silver eels. We also analyzed the regulation of ovarian vtgr expression throughout vitellogenesis in experimentally matured eels. The Vtg plasma level was measured by homologous ELISA experimental maturation. Our in silico search and phylogenetical analysis revealed a single vtgr in the European eel, orthologous to other vertebrate vtgr. The qRT-PCR studies revealed that vtgr is mainly expressed in the ovary and also detected in various other tissues such as brain, pituitary, gill, fat, heart, and testis, suggesting some extra-ovarian functions of VTGR. We showed that vtgr is expressed in ovaries of juvenile yellow eels with no higher expression in pre-pubertal silver eels nor in experimentally matured eels. This suggests that vtgr transcription already occurs during early pre-vitellogenesis of immature eels and is not further activated in vitellogenic oocytes. European eel Vtg plasma level increased throughout experimental maturation in agreement with previous studies. Taken together, these results suggest that vtgr transcript levels may not be a limiting step for the uptake of Vtg by the oocyte in the European eel. |
author2 |
Centre de Ressources Biologiques APHP-SU (PASS-CRB-APHP-SU) Unité Mixte de Service Production et Analyse de données en Sciences de la vie et en Santé (PASS) Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU) Biologie des Organismes et Ecosystèmes Aquatiques (BOREA) Université de Caen Normandie (UNICAEN) Normandie Université (NU)-Normandie Université (NU)-Muséum national d'Histoire naturelle (MNHN)-Institut de Recherche pour le Développement (IRD)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Université des Antilles (UA) |
format |
Article in Journal/Newspaper |
author |
Morini, M. Lafont, Anne-Gaelle Maugars, G. Baloche, S. Dufour, S. Asturiano, J. Perez, L. |
author_facet |
Morini, M. Lafont, Anne-Gaelle Maugars, G. Baloche, S. Dufour, S. Asturiano, J. Perez, L. |
author_sort |
Morini, M. |
title |
Identification and stable expression of vitellogenin receptor through vitellogenesis in the European eel |
title_short |
Identification and stable expression of vitellogenin receptor through vitellogenesis in the European eel |
title_full |
Identification and stable expression of vitellogenin receptor through vitellogenesis in the European eel |
title_fullStr |
Identification and stable expression of vitellogenin receptor through vitellogenesis in the European eel |
title_full_unstemmed |
Identification and stable expression of vitellogenin receptor through vitellogenesis in the European eel |
title_sort |
identification and stable expression of vitellogenin receptor through vitellogenesis in the european eel |
publisher |
HAL CCSD |
publishDate |
2020 |
url |
https://hal.science/hal-02989391 https://doi.org/10.1017/S1751731119003355 |
genre |
Anguilla anguilla |
genre_facet |
Anguilla anguilla |
op_source |
ISSN: 1751-7311 EISSN: 1751-732X Animal https://hal.science/hal-02989391 Animal, 2020, 14 (6), pp.1213-1222. ⟨10.1017/S1751731119003355⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1017/S1751731119003355 info:eu-repo/semantics/altIdentifier/pmid/31971122 hal-02989391 https://hal.science/hal-02989391 doi:10.1017/S1751731119003355 PUBMED: 31971122 |
op_doi |
https://doi.org/10.1017/S1751731119003355 |
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Animal |
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14 |
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6 |
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1213 |
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1222 |
_version_ |
1810481531436662784 |