Culturable diversity of Arctic phytoplankton during pack ice melting
International audience Massive phytoplankton blooms develop at the Arctic ice edge, sometimes extending far under the pack ice. An extensive culturing effort was conducted before and during a phytoplankton bloom in Baffin Bay between April and July 2016. Different isolation strategies were applied,...
Published in: | Elementa: Science of the Anthropocene |
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ftsorbonneuniv:oai:HAL:hal-02557111v2 2024-09-15T17:57:00+00:00 Culturable diversity of Arctic phytoplankton during pack ice melting Ribeiro, Catherine Gérikas dos Santos, Adriana Lopes Gourvil, Priscillia Le Gall, Florence Marie, Dominique Tragin, Margot Probert, Ian Vaulot, Daniel Universidad Mayor de San Andrés (UMSA) ECOlogy of MArine Plankton (ECOMAP) Adaptation et diversité en milieu marin (ADMM) Centre National de la Recherche Scientifique (CNRS)-Station biologique de Roscoff = Roscoff Marine Station (SBR) Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Station biologique de Roscoff = Roscoff Marine Station (SBR) Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS) Nanyang Technological University Singapour Station biologique de Roscoff = Roscoff Marine Station (SBR) Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS) Fédération de recherche de Roscoff (FR2424) 2020 https://hal.sorbonne-universite.fr/hal-02557111 https://hal.sorbonne-universite.fr/hal-02557111v2/document https://hal.sorbonne-universite.fr/hal-02557111v2/file/401-6958-1-PB.pdf https://doi.org/10.1525/elementa.401 en eng HAL CCSD University of California Press info:eu-repo/semantics/altIdentifier/doi/10.1525/elementa.401 hal-02557111 https://hal.sorbonne-universite.fr/hal-02557111 https://hal.sorbonne-universite.fr/hal-02557111v2/document https://hal.sorbonne-universite.fr/hal-02557111v2/file/401-6958-1-PB.pdf doi:10.1525/elementa.401 info:eu-repo/semantics/OpenAccess EISSN: 2325-1026 Elementa: Science of the Anthropocene https://hal.sorbonne-universite.fr/hal-02557111 Elementa: Science of the Anthropocene, 2020, 8 (1), pp.6. ⟨10.1525/elementa.401⟩ Arctic diatoms Arctic flagellates Isolation techniques 18S rRNA Roscoff culture collection Sympagic phytoplankton communities [SDE.BE]Environmental Sciences/Biodiversity and Ecology info:eu-repo/semantics/article Journal articles 2020 ftsorbonneuniv https://doi.org/10.1525/elementa.401 2024-07-25T23:48:00Z International audience Massive phytoplankton blooms develop at the Arctic ice edge, sometimes extending far under the pack ice. An extensive culturing effort was conducted before and during a phytoplankton bloom in Baffin Bay between April and July 2016. Different isolation strategies were applied, including flow cytometry cell sorting, manual single cell pipetting, and serial dilution. Although all three techniques yielded the most common organisms, each technique retrieved specific taxa, highlighting the importance of using several methods to maximize the number and diversity of isolated strains. More than 1,000 cultures were obtained, characterized by 18S rRNA sequencing and optical microscopy, and de-replicated to a subset of 276 strains presented in this work. Strains grouped into 57 phylotypes defined by 100% 18S rRNA sequence similarity. These phylotypes spread across five divisions: Heterokontophyta, Chlorophyta, Cryptophyta, Haptophyta and Dinophyta. Diatoms were the most abundant group (193 strains), mostly represented by the genera Chaetoceros and Attheya. The genera Baffinella and Pyramimonas were the most abundant non-diatom nanoplankton strains, while Micromonas polaris dominated the picoplankton. Diversity at the class level was higher during the peak of the bloom. Potentially new species were isolated, in particular within the genera Navicula, Nitzschia, Coscinodiscus, Thalassiosira, Pyramimonas, Mantoniella and Isochrysis. Culturing efforts such as this one highlight the unexplored eukaryotic plankton diversity in the Arctic and provide a large number of strains for analyzing physiological and metabolic impacts in this changing environment. Article in Journal/Newspaper Baffin Bay Baffin Bay Baffin Phytoplankton HAL Sorbonne Université Elementa: Science of the Anthropocene 8 |
institution |
Open Polar |
collection |
HAL Sorbonne Université |
op_collection_id |
ftsorbonneuniv |
language |
English |
topic |
Arctic diatoms Arctic flagellates Isolation techniques 18S rRNA Roscoff culture collection Sympagic phytoplankton communities [SDE.BE]Environmental Sciences/Biodiversity and Ecology |
spellingShingle |
Arctic diatoms Arctic flagellates Isolation techniques 18S rRNA Roscoff culture collection Sympagic phytoplankton communities [SDE.BE]Environmental Sciences/Biodiversity and Ecology Ribeiro, Catherine Gérikas dos Santos, Adriana Lopes Gourvil, Priscillia Le Gall, Florence Marie, Dominique Tragin, Margot Probert, Ian Vaulot, Daniel Culturable diversity of Arctic phytoplankton during pack ice melting |
topic_facet |
Arctic diatoms Arctic flagellates Isolation techniques 18S rRNA Roscoff culture collection Sympagic phytoplankton communities [SDE.BE]Environmental Sciences/Biodiversity and Ecology |
description |
International audience Massive phytoplankton blooms develop at the Arctic ice edge, sometimes extending far under the pack ice. An extensive culturing effort was conducted before and during a phytoplankton bloom in Baffin Bay between April and July 2016. Different isolation strategies were applied, including flow cytometry cell sorting, manual single cell pipetting, and serial dilution. Although all three techniques yielded the most common organisms, each technique retrieved specific taxa, highlighting the importance of using several methods to maximize the number and diversity of isolated strains. More than 1,000 cultures were obtained, characterized by 18S rRNA sequencing and optical microscopy, and de-replicated to a subset of 276 strains presented in this work. Strains grouped into 57 phylotypes defined by 100% 18S rRNA sequence similarity. These phylotypes spread across five divisions: Heterokontophyta, Chlorophyta, Cryptophyta, Haptophyta and Dinophyta. Diatoms were the most abundant group (193 strains), mostly represented by the genera Chaetoceros and Attheya. The genera Baffinella and Pyramimonas were the most abundant non-diatom nanoplankton strains, while Micromonas polaris dominated the picoplankton. Diversity at the class level was higher during the peak of the bloom. Potentially new species were isolated, in particular within the genera Navicula, Nitzschia, Coscinodiscus, Thalassiosira, Pyramimonas, Mantoniella and Isochrysis. Culturing efforts such as this one highlight the unexplored eukaryotic plankton diversity in the Arctic and provide a large number of strains for analyzing physiological and metabolic impacts in this changing environment. |
author2 |
Universidad Mayor de San Andrés (UMSA) ECOlogy of MArine Plankton (ECOMAP) Adaptation et diversité en milieu marin (ADMM) Centre National de la Recherche Scientifique (CNRS)-Station biologique de Roscoff = Roscoff Marine Station (SBR) Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Station biologique de Roscoff = Roscoff Marine Station (SBR) Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS) Nanyang Technological University Singapour Station biologique de Roscoff = Roscoff Marine Station (SBR) Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS) Fédération de recherche de Roscoff (FR2424) |
format |
Article in Journal/Newspaper |
author |
Ribeiro, Catherine Gérikas dos Santos, Adriana Lopes Gourvil, Priscillia Le Gall, Florence Marie, Dominique Tragin, Margot Probert, Ian Vaulot, Daniel |
author_facet |
Ribeiro, Catherine Gérikas dos Santos, Adriana Lopes Gourvil, Priscillia Le Gall, Florence Marie, Dominique Tragin, Margot Probert, Ian Vaulot, Daniel |
author_sort |
Ribeiro, Catherine Gérikas |
title |
Culturable diversity of Arctic phytoplankton during pack ice melting |
title_short |
Culturable diversity of Arctic phytoplankton during pack ice melting |
title_full |
Culturable diversity of Arctic phytoplankton during pack ice melting |
title_fullStr |
Culturable diversity of Arctic phytoplankton during pack ice melting |
title_full_unstemmed |
Culturable diversity of Arctic phytoplankton during pack ice melting |
title_sort |
culturable diversity of arctic phytoplankton during pack ice melting |
publisher |
HAL CCSD |
publishDate |
2020 |
url |
https://hal.sorbonne-universite.fr/hal-02557111 https://hal.sorbonne-universite.fr/hal-02557111v2/document https://hal.sorbonne-universite.fr/hal-02557111v2/file/401-6958-1-PB.pdf https://doi.org/10.1525/elementa.401 |
genre |
Baffin Bay Baffin Bay Baffin Phytoplankton |
genre_facet |
Baffin Bay Baffin Bay Baffin Phytoplankton |
op_source |
EISSN: 2325-1026 Elementa: Science of the Anthropocene https://hal.sorbonne-universite.fr/hal-02557111 Elementa: Science of the Anthropocene, 2020, 8 (1), pp.6. ⟨10.1525/elementa.401⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1525/elementa.401 hal-02557111 https://hal.sorbonne-universite.fr/hal-02557111 https://hal.sorbonne-universite.fr/hal-02557111v2/document https://hal.sorbonne-universite.fr/hal-02557111v2/file/401-6958-1-PB.pdf doi:10.1525/elementa.401 |
op_rights |
info:eu-repo/semantics/OpenAccess |
op_doi |
https://doi.org/10.1525/elementa.401 |
container_title |
Elementa: Science of the Anthropocene |
container_volume |
8 |
_version_ |
1810433199505932288 |