Proliferating cell nuclear antigen in gonad and associated storage tissue of the Pacific oyster Crassostrea gigas: seasonal immunodetection and expression in laser microdissected tissues
International audience To understand the processes involved in tissue remodeling associated with the seasonal reproductive cycle of the oyster Crassostrea gigas, we used immunodetection and expression measurements of proliferating cell nuclear antigen (PCNA). The expression of the PCNA gene was meas...
Published in: | Cell and Tissue Research |
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Online Access: | https://hal-normandie-univ.archives-ouvertes.fr/hal-02296538 https://doi.org/10.1007/s00441-009-0923-6 |
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ftsorbonneuniv:oai:HAL:hal-02296538v1 2023-05-15T15:57:46+02:00 Proliferating cell nuclear antigen in gonad and associated storage tissue of the Pacific oyster Crassostrea gigas: seasonal immunodetection and expression in laser microdissected tissues Franco, Alban Jouaux, Aude Mathieu, Michel Sourdaine, Pascal Lelong, Christophe Kellner, Kristell Heude Berthelin, Clothilde Physiologie et Ecophysiologie des Mollusques Marins (PE2M) Université de Caen Normandie (UNICAEN) Normandie Université (NU)-Normandie Université (NU)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Centre National de la Recherche Scientifique (CNRS) Interactions Cellules Organismes Environnement (ICORE) Normandie Université (NU)-Normandie Université (NU) 2010-04 https://hal-normandie-univ.archives-ouvertes.fr/hal-02296538 https://doi.org/10.1007/s00441-009-0923-6 en eng HAL CCSD Springer Verlag info:eu-repo/semantics/altIdentifier/doi/10.1007/s00441-009-0923-6 hal-02296538 https://hal-normandie-univ.archives-ouvertes.fr/hal-02296538 doi:10.1007/s00441-009-0923-6 ISSN: 0302-766X EISSN: 1432-0878 Cell and Tissue Research https://hal-normandie-univ.archives-ouvertes.fr/hal-02296538 Cell and Tissue Research, Springer Verlag, 2010, 340 (1), pp.201-210. ⟨10.1007/s00441-009-0923-6⟩ Proliferating cell nuclear antigen Reproduction Storage Laser microdissection Pacific oyster - Crassostrea gigas (Mollusca) [SDV]Life Sciences [q-bio] info:eu-repo/semantics/article Journal articles 2010 ftsorbonneuniv https://doi.org/10.1007/s00441-009-0923-6 2022-03-22T23:54:11Z International audience To understand the processes involved in tissue remodeling associated with the seasonal reproductive cycle of the oyster Crassostrea gigas, we used immunodetection and expression measurements of proliferating cell nuclear antigen (PCNA). The expression of the PCNA gene was measured by real-time polymerase chain reaction in the whole gonadal area compared with laser microdissected gonad and storage tissue. Results underlined the advantage of the laser microdissection approach to detect expression, mainly for early stages of spermatogenesis. In the storage tissue, PCNA expression was reduced in the gonadal tubules, but immunolabeled hemocytes and vesicular cells were detected when the storage tissue was being restored. In the gonadal tubules, the PCNA gene was more highly expressed in males than in females. As soon as spermatogenesis was initiated, PCNA expression showed a high and constant level. In females, the expression level increased gradually until the ripe stage. The immunological approach established the involvement of peritubular cells in gonadal tubule expansion during early gametogenesis. In both sexes, gonial mitosis was immunodetected throughout the reproductive cycle. In males, the occurrence of two types of spermatogonia was ascertained by differential immunolabeling, and intragonadal somatic cell proliferation was noted. As expected, immunolabeling was never observed from stage II spermatocytes to spermatozoa. In females, positively stained cells were detected from oogonia to growing oocytes with various labeled intracellular locations. Article in Journal/Newspaper Crassostrea gigas Pacific oyster HAL Sorbonne Université Pacific Cell and Tissue Research 340 1 201 210 |
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Open Polar |
collection |
HAL Sorbonne Université |
op_collection_id |
ftsorbonneuniv |
language |
English |
topic |
Proliferating cell nuclear antigen Reproduction Storage Laser microdissection Pacific oyster - Crassostrea gigas (Mollusca) [SDV]Life Sciences [q-bio] |
spellingShingle |
Proliferating cell nuclear antigen Reproduction Storage Laser microdissection Pacific oyster - Crassostrea gigas (Mollusca) [SDV]Life Sciences [q-bio] Franco, Alban Jouaux, Aude Mathieu, Michel Sourdaine, Pascal Lelong, Christophe Kellner, Kristell Heude Berthelin, Clothilde Proliferating cell nuclear antigen in gonad and associated storage tissue of the Pacific oyster Crassostrea gigas: seasonal immunodetection and expression in laser microdissected tissues |
topic_facet |
Proliferating cell nuclear antigen Reproduction Storage Laser microdissection Pacific oyster - Crassostrea gigas (Mollusca) [SDV]Life Sciences [q-bio] |
description |
International audience To understand the processes involved in tissue remodeling associated with the seasonal reproductive cycle of the oyster Crassostrea gigas, we used immunodetection and expression measurements of proliferating cell nuclear antigen (PCNA). The expression of the PCNA gene was measured by real-time polymerase chain reaction in the whole gonadal area compared with laser microdissected gonad and storage tissue. Results underlined the advantage of the laser microdissection approach to detect expression, mainly for early stages of spermatogenesis. In the storage tissue, PCNA expression was reduced in the gonadal tubules, but immunolabeled hemocytes and vesicular cells were detected when the storage tissue was being restored. In the gonadal tubules, the PCNA gene was more highly expressed in males than in females. As soon as spermatogenesis was initiated, PCNA expression showed a high and constant level. In females, the expression level increased gradually until the ripe stage. The immunological approach established the involvement of peritubular cells in gonadal tubule expansion during early gametogenesis. In both sexes, gonial mitosis was immunodetected throughout the reproductive cycle. In males, the occurrence of two types of spermatogonia was ascertained by differential immunolabeling, and intragonadal somatic cell proliferation was noted. As expected, immunolabeling was never observed from stage II spermatocytes to spermatozoa. In females, positively stained cells were detected from oogonia to growing oocytes with various labeled intracellular locations. |
author2 |
Physiologie et Ecophysiologie des Mollusques Marins (PE2M) Université de Caen Normandie (UNICAEN) Normandie Université (NU)-Normandie Université (NU)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Centre National de la Recherche Scientifique (CNRS) Interactions Cellules Organismes Environnement (ICORE) Normandie Université (NU)-Normandie Université (NU) |
format |
Article in Journal/Newspaper |
author |
Franco, Alban Jouaux, Aude Mathieu, Michel Sourdaine, Pascal Lelong, Christophe Kellner, Kristell Heude Berthelin, Clothilde |
author_facet |
Franco, Alban Jouaux, Aude Mathieu, Michel Sourdaine, Pascal Lelong, Christophe Kellner, Kristell Heude Berthelin, Clothilde |
author_sort |
Franco, Alban |
title |
Proliferating cell nuclear antigen in gonad and associated storage tissue of the Pacific oyster Crassostrea gigas: seasonal immunodetection and expression in laser microdissected tissues |
title_short |
Proliferating cell nuclear antigen in gonad and associated storage tissue of the Pacific oyster Crassostrea gigas: seasonal immunodetection and expression in laser microdissected tissues |
title_full |
Proliferating cell nuclear antigen in gonad and associated storage tissue of the Pacific oyster Crassostrea gigas: seasonal immunodetection and expression in laser microdissected tissues |
title_fullStr |
Proliferating cell nuclear antigen in gonad and associated storage tissue of the Pacific oyster Crassostrea gigas: seasonal immunodetection and expression in laser microdissected tissues |
title_full_unstemmed |
Proliferating cell nuclear antigen in gonad and associated storage tissue of the Pacific oyster Crassostrea gigas: seasonal immunodetection and expression in laser microdissected tissues |
title_sort |
proliferating cell nuclear antigen in gonad and associated storage tissue of the pacific oyster crassostrea gigas: seasonal immunodetection and expression in laser microdissected tissues |
publisher |
HAL CCSD |
publishDate |
2010 |
url |
https://hal-normandie-univ.archives-ouvertes.fr/hal-02296538 https://doi.org/10.1007/s00441-009-0923-6 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
ISSN: 0302-766X EISSN: 1432-0878 Cell and Tissue Research https://hal-normandie-univ.archives-ouvertes.fr/hal-02296538 Cell and Tissue Research, Springer Verlag, 2010, 340 (1), pp.201-210. ⟨10.1007/s00441-009-0923-6⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1007/s00441-009-0923-6 hal-02296538 https://hal-normandie-univ.archives-ouvertes.fr/hal-02296538 doi:10.1007/s00441-009-0923-6 |
op_doi |
https://doi.org/10.1007/s00441-009-0923-6 |
container_title |
Cell and Tissue Research |
container_volume |
340 |
container_issue |
1 |
container_start_page |
201 |
op_container_end_page |
210 |
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1766393462258663424 |