Morphological and molecular criteria allow the identification of putative germ stem cells in a lophotrochozoan, the Pacific oyster Crassostrea gigas
International audience While our knowledge of bivalve gametogenesis recently progressed, data on early stages of gametogenesis remain to be developed, especially when dealing with germinal stem cells (GSC) and their niche in these organisms. Here, we wish to develop a strategy to identify putative G...
Published in: | Histochemistry and Cell Biology |
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Online Access: | https://hal.science/hal-02142626 https://hal.science/hal-02142626/document https://hal.science/hal-02142626/file/Cherif-Feildel2018_Article_MorphologicalAndMolecularCrite_sans%20marque.pdf https://doi.org/10.1007/s00418-018-1740-3 |
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ftsorbonneuniv:oai:HAL:hal-02142626v1 2024-09-15T18:03:08+00:00 Morphological and molecular criteria allow the identification of putative germ stem cells in a lophotrochozoan, the Pacific oyster Crassostrea gigas Cherif-Feildel, Maëva Kellner, Kristell Goux, Didier Elie, Nicolas Adeline, Béatrice Lelong, Christophe Heude Berthelin, Clothilde Biologie des Organismes et Ecosystèmes Aquatiques (BOREA) Université de Caen Normandie (UNICAEN) Normandie Université (NU)-Normandie Université (NU)-Muséum national d'Histoire naturelle (MNHN)-Institut de Recherche pour le Développement (IRD)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Université des Antilles (UA) Interactions Cellules Organismes Environnement (ICORE) Normandie Université (NU)-Normandie Université (NU)-CHU Caen Normandie Université (NU)-Tumorothèque de Caen Basse-Normandie (TCBN)-Tumorothèque de Caen Basse-Normandie (TCBN) 2019-05 https://hal.science/hal-02142626 https://hal.science/hal-02142626/document https://hal.science/hal-02142626/file/Cherif-Feildel2018_Article_MorphologicalAndMolecularCrite_sans%20marque.pdf https://doi.org/10.1007/s00418-018-1740-3 en eng HAL CCSD Springer Verlag info:eu-repo/semantics/altIdentifier/doi/10.1007/s00418-018-1740-3 hal-02142626 https://hal.science/hal-02142626 https://hal.science/hal-02142626/document https://hal.science/hal-02142626/file/Cherif-Feildel2018_Article_MorphologicalAndMolecularCrite_sans%20marque.pdf doi:10.1007/s00418-018-1740-3 info:eu-repo/semantics/OpenAccess ISSN: 0948-6143 EISSN: 1432-119X Histochemistry and Cell Biology https://hal.science/hal-02142626 Histochemistry and Cell Biology, 2019, 151 (5), pp.419-433. ⟨10.1007/s00418-018-1740-3⟩ [SDV]Life Sciences [q-bio] [SDV.BDLR]Life Sciences [q-bio]/Reproductive Biology info:eu-repo/semantics/article Journal articles 2019 ftsorbonneuniv https://doi.org/10.1007/s00418-018-1740-3 2024-07-25T23:48:03Z International audience While our knowledge of bivalve gametogenesis recently progressed, data on early stages of gametogenesis remain to be developed, especially when dealing with germinal stem cells (GSC) and their niche in these organisms. Here, we wish to develop a strategy to identify putative GSC in Pacific oyster Crassostrea gigas based on morphological criteria combined with vasa marker expression. A histological quantitative approach, based on stereology, allowed us to identify two types of early germ cells in the germinal epithelium, one presenting round nuclei and the other irregular ones. Both early germ cell types present slightly condensed chromatin in nucleus, are vasa-positive and the Oyvlg (oyster vasa-like gene) expression in these cells is recorded throughout the whole gametogenesis process. The microenvironment of an early germ cell in oyster includes an associated somatic cell presenting an immunolabeling for BMP2/4 and a close myoid cell. In agreement with the GSC characteristics in other species, we postulate that putative germ stem cells in C. gigas correspond to the early germ cell type with irregular nucleus shape; those early germ cells with a round nucleus may consist in progenitors. Article in Journal/Newspaper Crassostrea gigas Pacific oyster HAL Sorbonne Université Histochemistry and Cell Biology 151 5 419 433 |
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Open Polar |
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HAL Sorbonne Université |
op_collection_id |
ftsorbonneuniv |
language |
English |
topic |
[SDV]Life Sciences [q-bio] [SDV.BDLR]Life Sciences [q-bio]/Reproductive Biology |
spellingShingle |
[SDV]Life Sciences [q-bio] [SDV.BDLR]Life Sciences [q-bio]/Reproductive Biology Cherif-Feildel, Maëva Kellner, Kristell Goux, Didier Elie, Nicolas Adeline, Béatrice Lelong, Christophe Heude Berthelin, Clothilde Morphological and molecular criteria allow the identification of putative germ stem cells in a lophotrochozoan, the Pacific oyster Crassostrea gigas |
topic_facet |
[SDV]Life Sciences [q-bio] [SDV.BDLR]Life Sciences [q-bio]/Reproductive Biology |
description |
International audience While our knowledge of bivalve gametogenesis recently progressed, data on early stages of gametogenesis remain to be developed, especially when dealing with germinal stem cells (GSC) and their niche in these organisms. Here, we wish to develop a strategy to identify putative GSC in Pacific oyster Crassostrea gigas based on morphological criteria combined with vasa marker expression. A histological quantitative approach, based on stereology, allowed us to identify two types of early germ cells in the germinal epithelium, one presenting round nuclei and the other irregular ones. Both early germ cell types present slightly condensed chromatin in nucleus, are vasa-positive and the Oyvlg (oyster vasa-like gene) expression in these cells is recorded throughout the whole gametogenesis process. The microenvironment of an early germ cell in oyster includes an associated somatic cell presenting an immunolabeling for BMP2/4 and a close myoid cell. In agreement with the GSC characteristics in other species, we postulate that putative germ stem cells in C. gigas correspond to the early germ cell type with irregular nucleus shape; those early germ cells with a round nucleus may consist in progenitors. |
author2 |
Biologie des Organismes et Ecosystèmes Aquatiques (BOREA) Université de Caen Normandie (UNICAEN) Normandie Université (NU)-Normandie Université (NU)-Muséum national d'Histoire naturelle (MNHN)-Institut de Recherche pour le Développement (IRD)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Université des Antilles (UA) Interactions Cellules Organismes Environnement (ICORE) Normandie Université (NU)-Normandie Université (NU)-CHU Caen Normandie Université (NU)-Tumorothèque de Caen Basse-Normandie (TCBN)-Tumorothèque de Caen Basse-Normandie (TCBN) |
format |
Article in Journal/Newspaper |
author |
Cherif-Feildel, Maëva Kellner, Kristell Goux, Didier Elie, Nicolas Adeline, Béatrice Lelong, Christophe Heude Berthelin, Clothilde |
author_facet |
Cherif-Feildel, Maëva Kellner, Kristell Goux, Didier Elie, Nicolas Adeline, Béatrice Lelong, Christophe Heude Berthelin, Clothilde |
author_sort |
Cherif-Feildel, Maëva |
title |
Morphological and molecular criteria allow the identification of putative germ stem cells in a lophotrochozoan, the Pacific oyster Crassostrea gigas |
title_short |
Morphological and molecular criteria allow the identification of putative germ stem cells in a lophotrochozoan, the Pacific oyster Crassostrea gigas |
title_full |
Morphological and molecular criteria allow the identification of putative germ stem cells in a lophotrochozoan, the Pacific oyster Crassostrea gigas |
title_fullStr |
Morphological and molecular criteria allow the identification of putative germ stem cells in a lophotrochozoan, the Pacific oyster Crassostrea gigas |
title_full_unstemmed |
Morphological and molecular criteria allow the identification of putative germ stem cells in a lophotrochozoan, the Pacific oyster Crassostrea gigas |
title_sort |
morphological and molecular criteria allow the identification of putative germ stem cells in a lophotrochozoan, the pacific oyster crassostrea gigas |
publisher |
HAL CCSD |
publishDate |
2019 |
url |
https://hal.science/hal-02142626 https://hal.science/hal-02142626/document https://hal.science/hal-02142626/file/Cherif-Feildel2018_Article_MorphologicalAndMolecularCrite_sans%20marque.pdf https://doi.org/10.1007/s00418-018-1740-3 |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
ISSN: 0948-6143 EISSN: 1432-119X Histochemistry and Cell Biology https://hal.science/hal-02142626 Histochemistry and Cell Biology, 2019, 151 (5), pp.419-433. ⟨10.1007/s00418-018-1740-3⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1007/s00418-018-1740-3 hal-02142626 https://hal.science/hal-02142626 https://hal.science/hal-02142626/document https://hal.science/hal-02142626/file/Cherif-Feildel2018_Article_MorphologicalAndMolecularCrite_sans%20marque.pdf doi:10.1007/s00418-018-1740-3 |
op_rights |
info:eu-repo/semantics/OpenAccess |
op_doi |
https://doi.org/10.1007/s00418-018-1740-3 |
container_title |
Histochemistry and Cell Biology |
container_volume |
151 |
container_issue |
5 |
container_start_page |
419 |
op_container_end_page |
433 |
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1810440648653799424 |