Molecular evolution and functional characterisation of insulin related peptides in molluscs: Contributions of Crassostrea gigas genomic and transcriptomic-wide screening
International audience Insulin Related Peptides (IRPs) belong to the insulin superfamily and possess a typical structure with two chains, B and A, linked by disulphide bonds. As the sequence conservation is usually low between members, IRPs are classified according to the number and position of thei...
Published in: | General and Comparative Endocrinology |
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Online Access: | https://hal.science/hal-02142617 https://doi.org/10.1016/j.ygcen.2018.10.019 |
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ftsorbonneuniv:oai:HAL:hal-02142617v1 2024-09-15T18:03:07+00:00 Molecular evolution and functional characterisation of insulin related peptides in molluscs: Contributions of Crassostrea gigas genomic and transcriptomic-wide screening Cherif-Feildel, Maëva Heude Berthelin, Clothilde Adeline, Béatrice Favrel, Pascal Kellner, Kristell Rivière, Guillaume Biologie des Organismes et Ecosystèmes Aquatiques (BOREA) Université de Caen Normandie (UNICAEN) Normandie Université (NU)-Normandie Université (NU)-Muséum national d'Histoire naturelle (MNHN)-Institut de Recherche pour le Développement (IRD)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Université des Antilles (UA) 2019-01 https://hal.science/hal-02142617 https://doi.org/10.1016/j.ygcen.2018.10.019 en eng HAL CCSD Elsevier info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ygcen.2018.10.019 hal-02142617 https://hal.science/hal-02142617 doi:10.1016/j.ygcen.2018.10.019 ISSN: 0016-6480 EISSN: 1095-6840 General and Comparative Endocrinology https://hal.science/hal-02142617 General and Comparative Endocrinology, 2019, 271, pp.15-29. ⟨10.1016/j.ygcen.2018.10.019⟩ Gametogenesis Phylogeny Molluscs Crassostrea gigas Insulin related peptides [SDV]Life Sciences [q-bio] [SDV.BDLR]Life Sciences [q-bio]/Reproductive Biology info:eu-repo/semantics/article Journal articles 2019 ftsorbonneuniv https://doi.org/10.1016/j.ygcen.2018.10.019 2024-07-25T23:48:03Z International audience Insulin Related Peptides (IRPs) belong to the insulin superfamily and possess a typical structure with two chains, B and A, linked by disulphide bonds. As the sequence conservation is usually low between members, IRPs are classified according to the number and position of their disulphide bonds. In molluscan species, the first IRPs identified, named Molluscan Insulin-related Peptides (MIPs), exhibit four disulphide bonds. The genomic and transcriptomic data screening in the Pacific oyster Crassostrea gigas (Mollusc, Bivalvia) allowed us to identify six IRP sequences belonging to three structural groups. Cg-MIP1 to 4 have the typical structure of MIPs with four disulphide bonds. Cg-ILP has three disulphide bonds like vertebrate Insulin-Like Peptides (ILPs). The last one, Cg-MILP7 has a significant homology with Drosophila ILP7 (DILP7) associated with two additional cysteines allowing the formation of a fourth disulphide bond. The phylogenetic analysis points out that ILPs may be the most ancestral form. Moreover, it appears that ILP7 orthologs are probably anterior to lophotrochozoa and ecdysozoa segregation. In order to investigate the diversity of physiological functions of the oyster IRPs, we combine in silico expression data, qPCR measurements and in situ hybridization. The Cg-ilp transcript, mainly detected in the digestive gland and in the gonadal area, is potentially involved in the control of digestion and gametogenesis. The expression of Cg-mip4 is mainly associated with the larval development. The Cg-mip transcript shared by the Cg-MIP1, 2 and 3, is mainly expressed in visceral ganglia but its expression was also observed in the gonads of mature males. This pattern suggested the key roles of IRPs in the control of sexual reproduction in molluscan species. Article in Journal/Newspaper Crassostrea gigas Pacific oyster HAL Sorbonne Université General and Comparative Endocrinology 271 15 29 |
institution |
Open Polar |
collection |
HAL Sorbonne Université |
op_collection_id |
ftsorbonneuniv |
language |
English |
topic |
Gametogenesis Phylogeny Molluscs Crassostrea gigas Insulin related peptides [SDV]Life Sciences [q-bio] [SDV.BDLR]Life Sciences [q-bio]/Reproductive Biology |
spellingShingle |
Gametogenesis Phylogeny Molluscs Crassostrea gigas Insulin related peptides [SDV]Life Sciences [q-bio] [SDV.BDLR]Life Sciences [q-bio]/Reproductive Biology Cherif-Feildel, Maëva Heude Berthelin, Clothilde Adeline, Béatrice Favrel, Pascal Kellner, Kristell Rivière, Guillaume Molecular evolution and functional characterisation of insulin related peptides in molluscs: Contributions of Crassostrea gigas genomic and transcriptomic-wide screening |
topic_facet |
Gametogenesis Phylogeny Molluscs Crassostrea gigas Insulin related peptides [SDV]Life Sciences [q-bio] [SDV.BDLR]Life Sciences [q-bio]/Reproductive Biology |
description |
International audience Insulin Related Peptides (IRPs) belong to the insulin superfamily and possess a typical structure with two chains, B and A, linked by disulphide bonds. As the sequence conservation is usually low between members, IRPs are classified according to the number and position of their disulphide bonds. In molluscan species, the first IRPs identified, named Molluscan Insulin-related Peptides (MIPs), exhibit four disulphide bonds. The genomic and transcriptomic data screening in the Pacific oyster Crassostrea gigas (Mollusc, Bivalvia) allowed us to identify six IRP sequences belonging to three structural groups. Cg-MIP1 to 4 have the typical structure of MIPs with four disulphide bonds. Cg-ILP has three disulphide bonds like vertebrate Insulin-Like Peptides (ILPs). The last one, Cg-MILP7 has a significant homology with Drosophila ILP7 (DILP7) associated with two additional cysteines allowing the formation of a fourth disulphide bond. The phylogenetic analysis points out that ILPs may be the most ancestral form. Moreover, it appears that ILP7 orthologs are probably anterior to lophotrochozoa and ecdysozoa segregation. In order to investigate the diversity of physiological functions of the oyster IRPs, we combine in silico expression data, qPCR measurements and in situ hybridization. The Cg-ilp transcript, mainly detected in the digestive gland and in the gonadal area, is potentially involved in the control of digestion and gametogenesis. The expression of Cg-mip4 is mainly associated with the larval development. The Cg-mip transcript shared by the Cg-MIP1, 2 and 3, is mainly expressed in visceral ganglia but its expression was also observed in the gonads of mature males. This pattern suggested the key roles of IRPs in the control of sexual reproduction in molluscan species. |
author2 |
Biologie des Organismes et Ecosystèmes Aquatiques (BOREA) Université de Caen Normandie (UNICAEN) Normandie Université (NU)-Normandie Université (NU)-Muséum national d'Histoire naturelle (MNHN)-Institut de Recherche pour le Développement (IRD)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Université des Antilles (UA) |
format |
Article in Journal/Newspaper |
author |
Cherif-Feildel, Maëva Heude Berthelin, Clothilde Adeline, Béatrice Favrel, Pascal Kellner, Kristell Rivière, Guillaume |
author_facet |
Cherif-Feildel, Maëva Heude Berthelin, Clothilde Adeline, Béatrice Favrel, Pascal Kellner, Kristell Rivière, Guillaume |
author_sort |
Cherif-Feildel, Maëva |
title |
Molecular evolution and functional characterisation of insulin related peptides in molluscs: Contributions of Crassostrea gigas genomic and transcriptomic-wide screening |
title_short |
Molecular evolution and functional characterisation of insulin related peptides in molluscs: Contributions of Crassostrea gigas genomic and transcriptomic-wide screening |
title_full |
Molecular evolution and functional characterisation of insulin related peptides in molluscs: Contributions of Crassostrea gigas genomic and transcriptomic-wide screening |
title_fullStr |
Molecular evolution and functional characterisation of insulin related peptides in molluscs: Contributions of Crassostrea gigas genomic and transcriptomic-wide screening |
title_full_unstemmed |
Molecular evolution and functional characterisation of insulin related peptides in molluscs: Contributions of Crassostrea gigas genomic and transcriptomic-wide screening |
title_sort |
molecular evolution and functional characterisation of insulin related peptides in molluscs: contributions of crassostrea gigas genomic and transcriptomic-wide screening |
publisher |
HAL CCSD |
publishDate |
2019 |
url |
https://hal.science/hal-02142617 https://doi.org/10.1016/j.ygcen.2018.10.019 |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
ISSN: 0016-6480 EISSN: 1095-6840 General and Comparative Endocrinology https://hal.science/hal-02142617 General and Comparative Endocrinology, 2019, 271, pp.15-29. ⟨10.1016/j.ygcen.2018.10.019⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ygcen.2018.10.019 hal-02142617 https://hal.science/hal-02142617 doi:10.1016/j.ygcen.2018.10.019 |
op_doi |
https://doi.org/10.1016/j.ygcen.2018.10.019 |
container_title |
General and Comparative Endocrinology |
container_volume |
271 |
container_start_page |
15 |
op_container_end_page |
29 |
_version_ |
1810440632191156224 |