Effects of Biostimulation on Growth of Indigenous Bacteria in Sub-Antarctic Soil Contamined with Oil Hydrocarbons
International audience In order to evaluate the efficiency of biostimulation of soil contaminated with oil hydrocarbons under sub-Antarctic conditions, a mesocosm study was initiated in May 2001 in the Kerguelen Archipelago (49°21¡¦S, 70°13¡¦E). The effects of temperature and fertilizer addition (In...
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ftsorbonneuniv:oai:HAL:hal-02043879v1 2024-05-12T07:54:29+00:00 Effects of Biostimulation on Growth of Indigenous Bacteria in Sub-Antarctic Soil Contamined with Oil Hydrocarbons Coulon, F. Delille, D. Laboratoire d'Océanographie Microbienne (LOMIC) Institut national des sciences de l'Univers (INSU - CNRS)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Observatoire océanologique de Banyuls (OOB) Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) 2003-07 https://hal.science/hal-02043879 https://hal.science/hal-02043879/document https://hal.science/hal-02043879/file/coulon_v58n4.pdf https://doi.org/10.2516/ogst:2003030 en eng HAL CCSD Institut Français du Pétrole (IFP) info:eu-repo/semantics/altIdentifier/doi/10.2516/ogst:2003030 hal-02043879 https://hal.science/hal-02043879 https://hal.science/hal-02043879/document https://hal.science/hal-02043879/file/coulon_v58n4.pdf doi:10.2516/ogst:2003030 info:eu-repo/semantics/OpenAccess ISSN: 1294-4475 EISSN: 1953-8189 Oil & Gas Science and Technology - Revue d'IFP Energies nouvelles https://hal.science/hal-02043879 Oil & Gas Science and Technology - Revue d'IFP Energies nouvelles, 2003, 58 (4), pp.469-479. ⟨10.2516/ogst:2003030⟩ [PHYS]Physics [physics] info:eu-repo/semantics/article Journal articles 2003 ftsorbonneuniv https://doi.org/10.2516/ogst:2003030 2024-04-18T03:45:10Z International audience In order to evaluate the efficiency of biostimulation of soil contaminated with oil hydrocarbons under sub-Antarctic conditions, a mesocosm study was initiated in May 2001 in the Kerguelen Archipelago (49°21¡¦S, 70°13¡¦E). The effects of temperature and fertilizer addition (Inipol EAP-22, Elf Atochem) on soil bacterial assemblages contaminated with hydrocarbons were studied in 6-l batches of subantarctic soil incubated in the dark. Six different conditions were used at three temperatures (4, 10 and 20°C): control, fertilizer (50 ml), diesel oil (100 ml), diesel oil (100 ml) + fertilizer (50 ml), ¡§Arabian light¡¨ crude oil (100 ml) and crude oil (100 ml) + fertilizer (50 ml). Mesocosms were sampled on a regular basis over a seven-month period. All samples were analyzed for total bacteria, viable heterotrophic assemblages and hydrocarbon-utilising microflora. The results clearly showed a significant response of sub-Antarctic microbial soil communities to hydrocarbon contamination. Large increases in total, heterotrophic and hydrocarbon-utilising bacteria were observed (from less than 5 × 105 MPN g-1 to more than 108 MPN g-1 for hydrocarbon degrading bacteria). Temperature elevation had no significant impact on the total or heterotrophic assemblages but induced a one order of magnitude increase in hydrocarbon-utilising bacteria in contaminated mesocosms. In contrast, fertilizer addition had no clear effect on hydrocarbon-degrading specific bacteria but stimulated heterotrophic growth in diesel oil-contaminated soils. Article in Journal/Newspaper Antarc* Antarctic HAL Sorbonne Université Antarctic Kerguelen |
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Open Polar |
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HAL Sorbonne Université |
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ftsorbonneuniv |
language |
English |
topic |
[PHYS]Physics [physics] |
spellingShingle |
[PHYS]Physics [physics] Coulon, F. Delille, D. Effects of Biostimulation on Growth of Indigenous Bacteria in Sub-Antarctic Soil Contamined with Oil Hydrocarbons |
topic_facet |
[PHYS]Physics [physics] |
description |
International audience In order to evaluate the efficiency of biostimulation of soil contaminated with oil hydrocarbons under sub-Antarctic conditions, a mesocosm study was initiated in May 2001 in the Kerguelen Archipelago (49°21¡¦S, 70°13¡¦E). The effects of temperature and fertilizer addition (Inipol EAP-22, Elf Atochem) on soil bacterial assemblages contaminated with hydrocarbons were studied in 6-l batches of subantarctic soil incubated in the dark. Six different conditions were used at three temperatures (4, 10 and 20°C): control, fertilizer (50 ml), diesel oil (100 ml), diesel oil (100 ml) + fertilizer (50 ml), ¡§Arabian light¡¨ crude oil (100 ml) and crude oil (100 ml) + fertilizer (50 ml). Mesocosms were sampled on a regular basis over a seven-month period. All samples were analyzed for total bacteria, viable heterotrophic assemblages and hydrocarbon-utilising microflora. The results clearly showed a significant response of sub-Antarctic microbial soil communities to hydrocarbon contamination. Large increases in total, heterotrophic and hydrocarbon-utilising bacteria were observed (from less than 5 × 105 MPN g-1 to more than 108 MPN g-1 for hydrocarbon degrading bacteria). Temperature elevation had no significant impact on the total or heterotrophic assemblages but induced a one order of magnitude increase in hydrocarbon-utilising bacteria in contaminated mesocosms. In contrast, fertilizer addition had no clear effect on hydrocarbon-degrading specific bacteria but stimulated heterotrophic growth in diesel oil-contaminated soils. |
author2 |
Laboratoire d'Océanographie Microbienne (LOMIC) Institut national des sciences de l'Univers (INSU - CNRS)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Observatoire océanologique de Banyuls (OOB) Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) |
format |
Article in Journal/Newspaper |
author |
Coulon, F. Delille, D. |
author_facet |
Coulon, F. Delille, D. |
author_sort |
Coulon, F. |
title |
Effects of Biostimulation on Growth of Indigenous Bacteria in Sub-Antarctic Soil Contamined with Oil Hydrocarbons |
title_short |
Effects of Biostimulation on Growth of Indigenous Bacteria in Sub-Antarctic Soil Contamined with Oil Hydrocarbons |
title_full |
Effects of Biostimulation on Growth of Indigenous Bacteria in Sub-Antarctic Soil Contamined with Oil Hydrocarbons |
title_fullStr |
Effects of Biostimulation on Growth of Indigenous Bacteria in Sub-Antarctic Soil Contamined with Oil Hydrocarbons |
title_full_unstemmed |
Effects of Biostimulation on Growth of Indigenous Bacteria in Sub-Antarctic Soil Contamined with Oil Hydrocarbons |
title_sort |
effects of biostimulation on growth of indigenous bacteria in sub-antarctic soil contamined with oil hydrocarbons |
publisher |
HAL CCSD |
publishDate |
2003 |
url |
https://hal.science/hal-02043879 https://hal.science/hal-02043879/document https://hal.science/hal-02043879/file/coulon_v58n4.pdf https://doi.org/10.2516/ogst:2003030 |
geographic |
Antarctic Kerguelen |
geographic_facet |
Antarctic Kerguelen |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_source |
ISSN: 1294-4475 EISSN: 1953-8189 Oil & Gas Science and Technology - Revue d'IFP Energies nouvelles https://hal.science/hal-02043879 Oil & Gas Science and Technology - Revue d'IFP Energies nouvelles, 2003, 58 (4), pp.469-479. ⟨10.2516/ogst:2003030⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.2516/ogst:2003030 hal-02043879 https://hal.science/hal-02043879 https://hal.science/hal-02043879/document https://hal.science/hal-02043879/file/coulon_v58n4.pdf doi:10.2516/ogst:2003030 |
op_rights |
info:eu-repo/semantics/OpenAccess |
op_doi |
https://doi.org/10.2516/ogst:2003030 |
_version_ |
1798849665225981952 |