Characterization of newly developed expressed sequence tag-derived microsatellite markers revealed low genetic diversity within and low connectivity between European Saccharina latissima populations
International audience The kelp Saccharina latissima is a species of high ecological and economic importance. We developed a novel set of S. latissima-specific genetic markers that will find applications in conservation biology, biodiversity assessment, and commercial exploitation of this macroalga....
Published in: | Journal of Applied Phycology |
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Main Authors: | , , , |
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Format: | Article in Journal/Newspaper |
Language: | English |
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HAL CCSD
2016
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Online Access: | https://hal.sorbonne-universite.fr/hal-01297556 https://hal.sorbonne-universite.fr/hal-01297556/document https://hal.sorbonne-universite.fr/hal-01297556/file/Guzinski_2016_Characterization_of.pdf https://doi.org/10.1007/s10811-016-0806-7 |
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ftsorbonneuniv:oai:HAL:hal-01297556v1 |
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Open Polar |
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HAL Sorbonne Université |
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ftsorbonneuniv |
language |
English |
topic |
Saccharina latissima Phaeophyceae NE Atlantic Population genetic structure Simple sequence repeat [SDV.EE.IEO]Life Sciences [q-bio]/Ecology environment/Symbiosis |
spellingShingle |
Saccharina latissima Phaeophyceae NE Atlantic Population genetic structure Simple sequence repeat [SDV.EE.IEO]Life Sciences [q-bio]/Ecology environment/Symbiosis Guzinski, Jaromir Mauger, Stéphane Cock, J. Mark Valero, Myriam Characterization of newly developed expressed sequence tag-derived microsatellite markers revealed low genetic diversity within and low connectivity between European Saccharina latissima populations |
topic_facet |
Saccharina latissima Phaeophyceae NE Atlantic Population genetic structure Simple sequence repeat [SDV.EE.IEO]Life Sciences [q-bio]/Ecology environment/Symbiosis |
description |
International audience The kelp Saccharina latissima is a species of high ecological and economic importance. We developed a novel set of S. latissima-specific genetic markers that will find applications in conservation biology, biodiversity assessment, and commercial exploitation of this macroalga. Thirty-two expressed sequence tag (EST)-derived microsatellite markers (SSRs) were developed and characterized in this study using publically available EST sequences. Twenty-seven percent of the 7064 analyzed ESTs contained repeat motifs, and polymerase chain reaction (PCR) amplification primers were designed for 96 selected loci. Fifty-one (53 %) of the primer pairs amplified their target loci, of which 32 (33 %) were polymorphic within a sample of 96 S. latissima sporophytes collected from six localities distributed along the European Atlantic coast from Southern Brittany (France) to Spitzbergen (Norway). The 32 loci harbored moderate levels of polymorphism with 2–13 alleles per locus (mean 5.4). The 25 loci that were retained for population genetic analyses revealed substantial genetic differentiation among the European populations (pairwise F ST values ranging from 0.077 to 0.562) that did not follow any pattern of isolation by distance. In addition, within-population genetic diversity was generally low (Hs < 0.323). Two non-mutually exclusive hypotheses were proposed to explain this low diversity pattern: (1) lower variability of the EST-derived microsatellites compared to the random distribution of SSRs developed from genomic DNA since the former are frequently located in coding regions, which are generally less variable, or (2) reduced effective population size of S. latissima. The particularly high genetic differentiation between the French and Scandinavian S. latissima populations is in agreement with the reported ecotypic differentiation, which may reflect an important resource for genetic improvement. The pattern of genetic diversity revealed in this study thus suggests that care should be taken to ... |
author2 |
Evolutionary Biology and Ecology of Algae (EBEA) Station biologique de Roscoff Roscoff (SBR) Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Pontificia Universidad Católica de Chile (UC)-Universidad Austral de Chile-Centre National de la Recherche Scientifique (CNRS) DIVersité et COnnectivité dans le paysage marin côtier (DIVCO) Adaptation et diversité en milieu marin (AD2M) Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Station biologique de Roscoff Roscoff (SBR) Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Laboratoire de Biologie Intégrative des Modèles Marins (LBI2M) ANR-10-BTBR-0004,IDEALG,Biotechnologies pour la valorisation des macroalgues(2010) |
format |
Article in Journal/Newspaper |
author |
Guzinski, Jaromir Mauger, Stéphane Cock, J. Mark Valero, Myriam |
author_facet |
Guzinski, Jaromir Mauger, Stéphane Cock, J. Mark Valero, Myriam |
author_sort |
Guzinski, Jaromir |
title |
Characterization of newly developed expressed sequence tag-derived microsatellite markers revealed low genetic diversity within and low connectivity between European Saccharina latissima populations |
title_short |
Characterization of newly developed expressed sequence tag-derived microsatellite markers revealed low genetic diversity within and low connectivity between European Saccharina latissima populations |
title_full |
Characterization of newly developed expressed sequence tag-derived microsatellite markers revealed low genetic diversity within and low connectivity between European Saccharina latissima populations |
title_fullStr |
Characterization of newly developed expressed sequence tag-derived microsatellite markers revealed low genetic diversity within and low connectivity between European Saccharina latissima populations |
title_full_unstemmed |
Characterization of newly developed expressed sequence tag-derived microsatellite markers revealed low genetic diversity within and low connectivity between European Saccharina latissima populations |
title_sort |
characterization of newly developed expressed sequence tag-derived microsatellite markers revealed low genetic diversity within and low connectivity between european saccharina latissima populations |
publisher |
HAL CCSD |
publishDate |
2016 |
url |
https://hal.sorbonne-universite.fr/hal-01297556 https://hal.sorbonne-universite.fr/hal-01297556/document https://hal.sorbonne-universite.fr/hal-01297556/file/Guzinski_2016_Characterization_of.pdf https://doi.org/10.1007/s10811-016-0806-7 |
genre |
Spitzbergen |
genre_facet |
Spitzbergen |
op_source |
ISSN: 0921-8971 EISSN: 1573-5176 Journal of Applied Phycology https://hal.sorbonne-universite.fr/hal-01297556 Journal of Applied Phycology, 2016, 28 (5), pp.3057-3070. ⟨10.1007/s10811-016-0806-7⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1007/s10811-016-0806-7 hal-01297556 https://hal.sorbonne-universite.fr/hal-01297556 https://hal.sorbonne-universite.fr/hal-01297556/document https://hal.sorbonne-universite.fr/hal-01297556/file/Guzinski_2016_Characterization_of.pdf doi:10.1007/s10811-016-0806-7 |
op_rights |
info:eu-repo/semantics/OpenAccess |
op_doi |
https://doi.org/10.1007/s10811-016-0806-7 |
container_title |
Journal of Applied Phycology |
container_volume |
28 |
container_issue |
5 |
container_start_page |
3057 |
op_container_end_page |
3070 |
_version_ |
1810482057053208576 |
spelling |
ftsorbonneuniv:oai:HAL:hal-01297556v1 2024-09-15T18:37:42+00:00 Characterization of newly developed expressed sequence tag-derived microsatellite markers revealed low genetic diversity within and low connectivity between European Saccharina latissima populations Guzinski, Jaromir Mauger, Stéphane Cock, J. Mark Valero, Myriam Evolutionary Biology and Ecology of Algae (EBEA) Station biologique de Roscoff Roscoff (SBR) Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Pontificia Universidad Católica de Chile (UC)-Universidad Austral de Chile-Centre National de la Recherche Scientifique (CNRS) DIVersité et COnnectivité dans le paysage marin côtier (DIVCO) Adaptation et diversité en milieu marin (AD2M) Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Station biologique de Roscoff Roscoff (SBR) Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Laboratoire de Biologie Intégrative des Modèles Marins (LBI2M) ANR-10-BTBR-0004,IDEALG,Biotechnologies pour la valorisation des macroalgues(2010) 2016 https://hal.sorbonne-universite.fr/hal-01297556 https://hal.sorbonne-universite.fr/hal-01297556/document https://hal.sorbonne-universite.fr/hal-01297556/file/Guzinski_2016_Characterization_of.pdf https://doi.org/10.1007/s10811-016-0806-7 en eng HAL CCSD Springer Verlag info:eu-repo/semantics/altIdentifier/doi/10.1007/s10811-016-0806-7 hal-01297556 https://hal.sorbonne-universite.fr/hal-01297556 https://hal.sorbonne-universite.fr/hal-01297556/document https://hal.sorbonne-universite.fr/hal-01297556/file/Guzinski_2016_Characterization_of.pdf doi:10.1007/s10811-016-0806-7 info:eu-repo/semantics/OpenAccess ISSN: 0921-8971 EISSN: 1573-5176 Journal of Applied Phycology https://hal.sorbonne-universite.fr/hal-01297556 Journal of Applied Phycology, 2016, 28 (5), pp.3057-3070. ⟨10.1007/s10811-016-0806-7⟩ Saccharina latissima Phaeophyceae NE Atlantic Population genetic structure Simple sequence repeat [SDV.EE.IEO]Life Sciences [q-bio]/Ecology environment/Symbiosis info:eu-repo/semantics/article Journal articles 2016 ftsorbonneuniv https://doi.org/10.1007/s10811-016-0806-7 2024-06-27T23:33:14Z International audience The kelp Saccharina latissima is a species of high ecological and economic importance. We developed a novel set of S. latissima-specific genetic markers that will find applications in conservation biology, biodiversity assessment, and commercial exploitation of this macroalga. Thirty-two expressed sequence tag (EST)-derived microsatellite markers (SSRs) were developed and characterized in this study using publically available EST sequences. Twenty-seven percent of the 7064 analyzed ESTs contained repeat motifs, and polymerase chain reaction (PCR) amplification primers were designed for 96 selected loci. Fifty-one (53 %) of the primer pairs amplified their target loci, of which 32 (33 %) were polymorphic within a sample of 96 S. latissima sporophytes collected from six localities distributed along the European Atlantic coast from Southern Brittany (France) to Spitzbergen (Norway). The 32 loci harbored moderate levels of polymorphism with 2–13 alleles per locus (mean 5.4). The 25 loci that were retained for population genetic analyses revealed substantial genetic differentiation among the European populations (pairwise F ST values ranging from 0.077 to 0.562) that did not follow any pattern of isolation by distance. In addition, within-population genetic diversity was generally low (Hs < 0.323). Two non-mutually exclusive hypotheses were proposed to explain this low diversity pattern: (1) lower variability of the EST-derived microsatellites compared to the random distribution of SSRs developed from genomic DNA since the former are frequently located in coding regions, which are generally less variable, or (2) reduced effective population size of S. latissima. The particularly high genetic differentiation between the French and Scandinavian S. latissima populations is in agreement with the reported ecotypic differentiation, which may reflect an important resource for genetic improvement. The pattern of genetic diversity revealed in this study thus suggests that care should be taken to ... Article in Journal/Newspaper Spitzbergen HAL Sorbonne Université Journal of Applied Phycology 28 5 3057 3070 |