Early development and molecular plasticity in the Mediterranean sea urchin Paracentrotus lividus exposed to CO2-driven acidification
International audience Ocean acidification is predicted to have significant effects on benthic calcifying invertebrates, in particular on their early developmental stages. Echinoderm larvae could be particularly vulnerable to decreased pH, with major consequences for adult populations. The objective...
Published in: | Journal of Experimental Biology |
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Main Authors: | , , , , , , , , , , |
Other Authors: | , , , , , , , , , , , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
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HAL CCSD
2011
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Online Access: | https://hal.science/hal-01255970 https://doi.org/10.1242/jeb.051169 |
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ftsorbonneuniv:oai:HAL:hal-01255970v1 |
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institution |
Open Polar |
collection |
HAL Sorbonne Université |
op_collection_id |
ftsorbonneuniv |
language |
English |
topic |
early life stage sea urchin development calcification gene expression ocean acidification [SDV]Life Sciences [q-bio] [SDE]Environmental Sciences |
spellingShingle |
early life stage sea urchin development calcification gene expression ocean acidification [SDV]Life Sciences [q-bio] [SDE]Environmental Sciences Martin, Sophie Richier, Sophie Pedrotti, Maria-Luiza Dupont, Sam Castejon, Charlotte Gerakis, Yannis Kerros, Marie-Emmanuelle Oberhaensli, Francois Teyssié, Jean-Louis Jeffree, Ross Gattuso, Jean-Pierre Early development and molecular plasticity in the Mediterranean sea urchin Paracentrotus lividus exposed to CO2-driven acidification |
topic_facet |
early life stage sea urchin development calcification gene expression ocean acidification [SDV]Life Sciences [q-bio] [SDE]Environmental Sciences |
description |
International audience Ocean acidification is predicted to have significant effects on benthic calcifying invertebrates, in particular on their early developmental stages. Echinoderm larvae could be particularly vulnerable to decreased pH, with major consequences for adult populations. The objective of this study was to understand how ocean acidification would affect the initial life stages of the sea urchin Paracentrotus lividus, a common species that is widely distributed in the Mediterranean Sea and the NE Atlantic. The effects of decreased pH (elevated P-CO2) were investigated through physiological and molecular analyses on both embryonic and larval stages. Eggs and larvae were reared in Mediterranean seawater at six pH levels, i.e. pH(T) 8.1, 7.9, 7.7, 7.5, 7.25 and 7.0. Fertilization success, survival, growth and calcification rates were monitored over a 3. day period. The expression of genes coding for key proteins involved in development and biomineralization was also monitored. Paracentrotus lividus appears to be extremely resistant to low pH, with no effect on fertilization success or larval survival. Larval growth was slowed when exposed to low pH but with no direct impact on relative larval morphology or calcification down to pHT. 7.25. Consequently, at a given time, larvae exposed to low pH were present at a normal but delayed larval stage. More surprisingly, candidate genes involved in development and biomineralization were upregulated by factors of up to 26 at low pH. Our results revealed plasticity at the gene expression level that allows a normal, but delayed, development under low pH conditions. |
author2 |
Ecogéochimie et Fonctionnement des Ecosystèmes Benthiques (EFEB) Adaptation et diversité en milieu marin (AD2M) Station biologique de Roscoff Roscoff (SBR) Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Station biologique de Roscoff Roscoff (SBR) Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Laboratoire d'océanographie de Villefranche (LOV) Observatoire océanologique de Villefranche-sur-mer (OOVM) Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Department of Marine Ecology Göteborgs Universitet = University of Gothenburg (GU) Croissance cellulaire, réparation et régénération tissulaires (CRRET) Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12)-Centre National de la Recherche Scientifique (CNRS) Marine Environment Laboratories Monaco (IAEA-MEL) International Atomic Energy Agency Vienna (IAEA) Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS) European Community 211384 Government of the Principality of Monaco Linnaeus Centre for Marine Evolutionary Biology at the University of Gothenburg Swedish Research Councils |
format |
Article in Journal/Newspaper |
author |
Martin, Sophie Richier, Sophie Pedrotti, Maria-Luiza Dupont, Sam Castejon, Charlotte Gerakis, Yannis Kerros, Marie-Emmanuelle Oberhaensli, Francois Teyssié, Jean-Louis Jeffree, Ross Gattuso, Jean-Pierre |
author_facet |
Martin, Sophie Richier, Sophie Pedrotti, Maria-Luiza Dupont, Sam Castejon, Charlotte Gerakis, Yannis Kerros, Marie-Emmanuelle Oberhaensli, Francois Teyssié, Jean-Louis Jeffree, Ross Gattuso, Jean-Pierre |
author_sort |
Martin, Sophie |
title |
Early development and molecular plasticity in the Mediterranean sea urchin Paracentrotus lividus exposed to CO2-driven acidification |
title_short |
Early development and molecular plasticity in the Mediterranean sea urchin Paracentrotus lividus exposed to CO2-driven acidification |
title_full |
Early development and molecular plasticity in the Mediterranean sea urchin Paracentrotus lividus exposed to CO2-driven acidification |
title_fullStr |
Early development and molecular plasticity in the Mediterranean sea urchin Paracentrotus lividus exposed to CO2-driven acidification |
title_full_unstemmed |
Early development and molecular plasticity in the Mediterranean sea urchin Paracentrotus lividus exposed to CO2-driven acidification |
title_sort |
early development and molecular plasticity in the mediterranean sea urchin paracentrotus lividus exposed to co2-driven acidification |
publisher |
HAL CCSD |
publishDate |
2011 |
url |
https://hal.science/hal-01255970 https://doi.org/10.1242/jeb.051169 |
genre |
Ocean acidification |
genre_facet |
Ocean acidification |
op_source |
ISSN: 0022-0949 EISSN: 1477-9145 Journal of Experimental Biology https://hal.science/hal-01255970 Journal of Experimental Biology, 2011, 214 (8), pp.1357-1368. ⟨10.1242/jeb.051169⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1242/jeb.051169 hal-01255970 https://hal.science/hal-01255970 doi:10.1242/jeb.051169 |
op_doi |
https://doi.org/10.1242/jeb.051169 |
container_title |
Journal of Experimental Biology |
container_volume |
214 |
container_issue |
8 |
container_start_page |
1357 |
op_container_end_page |
1368 |
_version_ |
1809932985780142080 |
spelling |
ftsorbonneuniv:oai:HAL:hal-01255970v1 2024-09-09T20:01:11+00:00 Early development and molecular plasticity in the Mediterranean sea urchin Paracentrotus lividus exposed to CO2-driven acidification Martin, Sophie Richier, Sophie Pedrotti, Maria-Luiza Dupont, Sam Castejon, Charlotte Gerakis, Yannis Kerros, Marie-Emmanuelle Oberhaensli, Francois Teyssié, Jean-Louis Jeffree, Ross Gattuso, Jean-Pierre Ecogéochimie et Fonctionnement des Ecosystèmes Benthiques (EFEB) Adaptation et diversité en milieu marin (AD2M) Station biologique de Roscoff Roscoff (SBR) Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Station biologique de Roscoff Roscoff (SBR) Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Laboratoire d'océanographie de Villefranche (LOV) Observatoire océanologique de Villefranche-sur-mer (OOVM) Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Department of Marine Ecology Göteborgs Universitet = University of Gothenburg (GU) Croissance cellulaire, réparation et régénération tissulaires (CRRET) Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12)-Centre National de la Recherche Scientifique (CNRS) Marine Environment Laboratories Monaco (IAEA-MEL) International Atomic Energy Agency Vienna (IAEA) Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS) European Community 211384 Government of the Principality of Monaco Linnaeus Centre for Marine Evolutionary Biology at the University of Gothenburg Swedish Research Councils 2011 https://hal.science/hal-01255970 https://doi.org/10.1242/jeb.051169 en eng HAL CCSD The Company of Biologists info:eu-repo/semantics/altIdentifier/doi/10.1242/jeb.051169 hal-01255970 https://hal.science/hal-01255970 doi:10.1242/jeb.051169 ISSN: 0022-0949 EISSN: 1477-9145 Journal of Experimental Biology https://hal.science/hal-01255970 Journal of Experimental Biology, 2011, 214 (8), pp.1357-1368. ⟨10.1242/jeb.051169⟩ early life stage sea urchin development calcification gene expression ocean acidification [SDV]Life Sciences [q-bio] [SDE]Environmental Sciences info:eu-repo/semantics/article Journal articles 2011 ftsorbonneuniv https://doi.org/10.1242/jeb.051169 2024-06-20T23:51:22Z International audience Ocean acidification is predicted to have significant effects on benthic calcifying invertebrates, in particular on their early developmental stages. Echinoderm larvae could be particularly vulnerable to decreased pH, with major consequences for adult populations. The objective of this study was to understand how ocean acidification would affect the initial life stages of the sea urchin Paracentrotus lividus, a common species that is widely distributed in the Mediterranean Sea and the NE Atlantic. The effects of decreased pH (elevated P-CO2) were investigated through physiological and molecular analyses on both embryonic and larval stages. Eggs and larvae were reared in Mediterranean seawater at six pH levels, i.e. pH(T) 8.1, 7.9, 7.7, 7.5, 7.25 and 7.0. Fertilization success, survival, growth and calcification rates were monitored over a 3. day period. The expression of genes coding for key proteins involved in development and biomineralization was also monitored. Paracentrotus lividus appears to be extremely resistant to low pH, with no effect on fertilization success or larval survival. Larval growth was slowed when exposed to low pH but with no direct impact on relative larval morphology or calcification down to pHT. 7.25. Consequently, at a given time, larvae exposed to low pH were present at a normal but delayed larval stage. More surprisingly, candidate genes involved in development and biomineralization were upregulated by factors of up to 26 at low pH. Our results revealed plasticity at the gene expression level that allows a normal, but delayed, development under low pH conditions. Article in Journal/Newspaper Ocean acidification HAL Sorbonne Université Journal of Experimental Biology 214 8 1357 1368 |