Transcriptome and Peptidome Characterisation of the Main Neuropeptides and Peptidic Hormones of a Euphausiid: The Ice Krill, Euphausia crystallorophias
International audience Background: The Ice krill, Euphausia crystallorophias is one of the species at the base of the Southern Ocean food chain. Given their significant contribution to the biomass of the Southern Ocean, it is vitally important to gain a better understanding of their physiology and,...
Published in: | PLoS ONE |
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Main Authors: | , , , , , , , |
Other Authors: | , , , , , , , , , , , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
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HAL CCSD
2013
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Subjects: | |
Online Access: | https://hal.science/hal-01250917 https://hal.science/hal-01250917/document https://hal.science/hal-01250917/file/journal.pone.0071609.pdf https://doi.org/10.1371/journal.pone.0071609 |
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HAL Sorbonne Université |
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English |
topic |
[SDV]Life Sciences [q-bio] [SDE]Environmental Sciences |
spellingShingle |
[SDV]Life Sciences [q-bio] [SDE]Environmental Sciences Toullec, Jean-Yves Corre, Erwan Bernay, Benoit Thorne, Michael A. S. Cascella, Kévin Ollivaux, Céline Henry, Joël Clark, Melody S. Transcriptome and Peptidome Characterisation of the Main Neuropeptides and Peptidic Hormones of a Euphausiid: The Ice Krill, Euphausia crystallorophias |
topic_facet |
[SDV]Life Sciences [q-bio] [SDE]Environmental Sciences |
description |
International audience Background: The Ice krill, Euphausia crystallorophias is one of the species at the base of the Southern Ocean food chain. Given their significant contribution to the biomass of the Southern Ocean, it is vitally important to gain a better understanding of their physiology and, in particular, anticipate their responses to climate change effects in the warming seas around Antarctica. Methodology/Principal Findings: Illumina sequencing was used to produce a transcriptome of the ice krill. Analysis of the assembled contigs via two different methods, produced 36 new pre-pro-peptides, coding for 61 neuropeptides or peptide hormones belonging to the following families: Allatostatins (A, B et C), Bursicon (alpha and beta), Crustacean Hyperglycemic Hormones (CHH and MIH/VIHs), Crustacean Cardioactive Peptide (CCAP), Corazonin, Diuretic Hormones (DH), the Eclosion Hormone (EH), Neuroparsin, Neuropeptide F (NPF), small Neuropeptide F (sNPF), Pigment Dispersing Hormone (PDH), Red Pigment Concentrating Hormone (RPCH) and finally Tachykinin. LC/MS/MS proteomics was also carried out on eyestalk extracts, which are the major site of neuropeptide synthesis in decapod crustaceans. Results confirmed the presence of six neuropeptides and six precursor-related peptides previously identified in the transcriptome analyses. Conclusions: This study represents the first comprehensive analysis of neuropeptide hormones in a Eucarida non-decapod Malacostraca, several of which are described for the first time in a non-decapod crustacean. Additionally, there is a potential expansion of PDH and Neuropeptide F family members, which may reflect certain life history traits such as circadian rhythms associated with diurnal migrations and also the confirmation via mass spectrometry of several novel pre-propeptides, of unknown function. Knowledge of these essential hormones provides a vital framework for understanding the physiological response of this key Southern Ocean species to climate change and provides a valuable ... |
author2 |
Adaptation et Biologie des Invertébrés en Conditions Extrêmes (ABICE) Adaptation et diversité en milieu marin (AD2M) Station biologique de Roscoff Roscoff (SBR) Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Station biologique de Roscoff Roscoff (SBR) Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Phophorylation de protéines et Pathologies Humaines (P3H) Biologie des mollusques marins et des écosystèmes associés (BioMEA) Université de Caen Normandie (UNICAEN) Normandie Université (NU)-Normandie Université (NU)-Centre National de la Recherche Scientifique (CNRS) British Antarctic Survey (BAS) Natural Environment Research Council (NERC) Laboratoire de Biologie Intégrative des Modèles Marins (LBI2M) Biologie des Organismes et Ecosystèmes Aquatiques (BOREA) Normandie Université (NU)-Normandie Université (NU)-Muséum national d'Histoire naturelle (MNHN)-Institut de Recherche pour le Développement (IRD)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Université des Antilles (UA) Emergence-UPMC research program Region Bretagne Institut Paul Emile Victor (KREVET program) la Region Bretagne (SAD-1 - DRAKAR program) Natural Environment Research Council |
format |
Article in Journal/Newspaper |
author |
Toullec, Jean-Yves Corre, Erwan Bernay, Benoit Thorne, Michael A. S. Cascella, Kévin Ollivaux, Céline Henry, Joël Clark, Melody S. |
author_facet |
Toullec, Jean-Yves Corre, Erwan Bernay, Benoit Thorne, Michael A. S. Cascella, Kévin Ollivaux, Céline Henry, Joël Clark, Melody S. |
author_sort |
Toullec, Jean-Yves |
title |
Transcriptome and Peptidome Characterisation of the Main Neuropeptides and Peptidic Hormones of a Euphausiid: The Ice Krill, Euphausia crystallorophias |
title_short |
Transcriptome and Peptidome Characterisation of the Main Neuropeptides and Peptidic Hormones of a Euphausiid: The Ice Krill, Euphausia crystallorophias |
title_full |
Transcriptome and Peptidome Characterisation of the Main Neuropeptides and Peptidic Hormones of a Euphausiid: The Ice Krill, Euphausia crystallorophias |
title_fullStr |
Transcriptome and Peptidome Characterisation of the Main Neuropeptides and Peptidic Hormones of a Euphausiid: The Ice Krill, Euphausia crystallorophias |
title_full_unstemmed |
Transcriptome and Peptidome Characterisation of the Main Neuropeptides and Peptidic Hormones of a Euphausiid: The Ice Krill, Euphausia crystallorophias |
title_sort |
transcriptome and peptidome characterisation of the main neuropeptides and peptidic hormones of a euphausiid: the ice krill, euphausia crystallorophias |
publisher |
HAL CCSD |
publishDate |
2013 |
url |
https://hal.science/hal-01250917 https://hal.science/hal-01250917/document https://hal.science/hal-01250917/file/journal.pone.0071609.pdf https://doi.org/10.1371/journal.pone.0071609 |
geographic |
Southern Ocean |
geographic_facet |
Southern Ocean |
genre |
Antarc* Antarctica Southern Ocean |
genre_facet |
Antarc* Antarctica Southern Ocean |
op_source |
ISSN: 1932-6203 EISSN: 1932-6203 PLoS ONE https://hal.science/hal-01250917 PLoS ONE, 2013, 8 (8), pp.e71609. ⟨10.1371/journal.pone.0071609⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0071609 hal-01250917 https://hal.science/hal-01250917 https://hal.science/hal-01250917/document https://hal.science/hal-01250917/file/journal.pone.0071609.pdf doi:10.1371/journal.pone.0071609 |
op_rights |
http://creativecommons.org/licenses/by/ info:eu-repo/semantics/OpenAccess |
op_doi |
https://doi.org/10.1371/journal.pone.0071609 |
container_title |
PLoS ONE |
container_volume |
8 |
container_issue |
8 |
container_start_page |
e71609 |
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1790594292384792576 |
spelling |
ftsorbonneuniv:oai:HAL:hal-01250917v1 2024-02-11T09:58:36+01:00 Transcriptome and Peptidome Characterisation of the Main Neuropeptides and Peptidic Hormones of a Euphausiid: The Ice Krill, Euphausia crystallorophias Toullec, Jean-Yves Corre, Erwan Bernay, Benoit Thorne, Michael A. S. Cascella, Kévin Ollivaux, Céline Henry, Joël Clark, Melody S. Adaptation et Biologie des Invertébrés en Conditions Extrêmes (ABICE) Adaptation et diversité en milieu marin (AD2M) Station biologique de Roscoff Roscoff (SBR) Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Station biologique de Roscoff Roscoff (SBR) Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Phophorylation de protéines et Pathologies Humaines (P3H) Biologie des mollusques marins et des écosystèmes associés (BioMEA) Université de Caen Normandie (UNICAEN) Normandie Université (NU)-Normandie Université (NU)-Centre National de la Recherche Scientifique (CNRS) British Antarctic Survey (BAS) Natural Environment Research Council (NERC) Laboratoire de Biologie Intégrative des Modèles Marins (LBI2M) Biologie des Organismes et Ecosystèmes Aquatiques (BOREA) Normandie Université (NU)-Normandie Université (NU)-Muséum national d'Histoire naturelle (MNHN)-Institut de Recherche pour le Développement (IRD)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Université des Antilles (UA) Emergence-UPMC research program Region Bretagne Institut Paul Emile Victor (KREVET program) la Region Bretagne (SAD-1 - DRAKAR program) Natural Environment Research Council 2013 https://hal.science/hal-01250917 https://hal.science/hal-01250917/document https://hal.science/hal-01250917/file/journal.pone.0071609.pdf https://doi.org/10.1371/journal.pone.0071609 en eng HAL CCSD Public Library of Science info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0071609 hal-01250917 https://hal.science/hal-01250917 https://hal.science/hal-01250917/document https://hal.science/hal-01250917/file/journal.pone.0071609.pdf doi:10.1371/journal.pone.0071609 http://creativecommons.org/licenses/by/ info:eu-repo/semantics/OpenAccess ISSN: 1932-6203 EISSN: 1932-6203 PLoS ONE https://hal.science/hal-01250917 PLoS ONE, 2013, 8 (8), pp.e71609. ⟨10.1371/journal.pone.0071609⟩ [SDV]Life Sciences [q-bio] [SDE]Environmental Sciences info:eu-repo/semantics/article Journal articles 2013 ftsorbonneuniv https://doi.org/10.1371/journal.pone.0071609 2024-01-23T23:57:25Z International audience Background: The Ice krill, Euphausia crystallorophias is one of the species at the base of the Southern Ocean food chain. Given their significant contribution to the biomass of the Southern Ocean, it is vitally important to gain a better understanding of their physiology and, in particular, anticipate their responses to climate change effects in the warming seas around Antarctica. Methodology/Principal Findings: Illumina sequencing was used to produce a transcriptome of the ice krill. Analysis of the assembled contigs via two different methods, produced 36 new pre-pro-peptides, coding for 61 neuropeptides or peptide hormones belonging to the following families: Allatostatins (A, B et C), Bursicon (alpha and beta), Crustacean Hyperglycemic Hormones (CHH and MIH/VIHs), Crustacean Cardioactive Peptide (CCAP), Corazonin, Diuretic Hormones (DH), the Eclosion Hormone (EH), Neuroparsin, Neuropeptide F (NPF), small Neuropeptide F (sNPF), Pigment Dispersing Hormone (PDH), Red Pigment Concentrating Hormone (RPCH) and finally Tachykinin. LC/MS/MS proteomics was also carried out on eyestalk extracts, which are the major site of neuropeptide synthesis in decapod crustaceans. Results confirmed the presence of six neuropeptides and six precursor-related peptides previously identified in the transcriptome analyses. Conclusions: This study represents the first comprehensive analysis of neuropeptide hormones in a Eucarida non-decapod Malacostraca, several of which are described for the first time in a non-decapod crustacean. Additionally, there is a potential expansion of PDH and Neuropeptide F family members, which may reflect certain life history traits such as circadian rhythms associated with diurnal migrations and also the confirmation via mass spectrometry of several novel pre-propeptides, of unknown function. Knowledge of these essential hormones provides a vital framework for understanding the physiological response of this key Southern Ocean species to climate change and provides a valuable ... Article in Journal/Newspaper Antarc* Antarctica Southern Ocean HAL Sorbonne Université Southern Ocean PLoS ONE 8 8 e71609 |