Next-generation transcriptome characterization in three Nacella species (Patellogastropoda: Nacellidae) from South America and Antarctica

• Published in: Marine Genomics
• Main Authors: Fuenzalida, Gonzalo, Poulin, Elie, Gonzalez-Wevar, Claudio, Molina, Cristian, Cardenas, Leyla
• Other Authors: Adaptation et Biologie des Invertébrés en Conditions Extrêmes (ABICE), Adaptation et diversité en milieu marin (AD2M), Station biologique de Roscoff Roscoff (SBR), Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Station biologique de Roscoff Roscoff (SBR), Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Instituto de Ecología y Biodiversidad, Instituto Antartico Chileno Inach T22-10, Millennium Nucleus Center for the Study of Multiple-drivers on Marine Socio-Ecological Systems (MUSELS) by MINECON NC120086
• Format: Article in Journal/Newspaper
• Language: English
• Published: HAL CCSD 2014
• Subjects: Pyrosequencing; Comparative transcriptome; Patellogastropods; Antarctica; South America; [SDV]Life Sciences [q-bio]; [SDE]Environmental Sciences; Antarctic; The Antarctic; Antarctic Peninsula; Patagonia; Nacella; Antarc*
• Online Access: https://hal.science/hal-01250909; https://doi.org/10.1016/j.margen.2014.06.004

International audience The southern tip of South America and Antarctica are particularly interesting due to many genera and also species currently sharing between both areas. The genus Nacella (Patellogastropoda: Nacellidae) is distributed in different regions of South America and Antarctica living preferentially on rocks and boulders and grazing on algae, diatoms and bacterial films. We described the transcriptomes of three Nacella species, Nacella concinna (Strebel, 1908), inhabiting the Antarctic Peninsula; Nacella magallanica (Gmelin, 1791), from Patagonia and Nacella clypeater (Lesson, 1831), from central Chile. In total, we obtained over 20,000 contigs with an average length of 583 bp. Homologous protein coding genes (PCGs) for mitochondrial genome of the three species were characterized and a database of molecular markers was also generated. This study represents the first publicly available report on pyrosequencing data for patellogastropod species, and provides an important comparative resource for studies in ecophysiology and evolutionary adaptation in marine invertebrate species. (c) 2014 Elsevier B.V. All rights reserved.