Water mass-specificity of bacterial communities in the North Atlantic revealed by massively parallel sequencing
International audience Bacterial assemblages from subsurface (100 m depth), meso- (200-1000 m depth) and bathy-pelagic (below 1000 m depth) zones at 10 stations along a North Atlantic Ocean transect from 60°N to 5°S were characterized using massively parallel pyrotag sequencing of the V6 region of t...
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ftsorbonneuniv:oai:HAL:hal-01086716v1 2024-06-16T07:41:43+00:00 Water mass-specificity of bacterial communities in the North Atlantic revealed by massively parallel sequencing Agogué, Hélène Lamy, Dominique Neal, Phillip, R Sogin, Mitchell, S Herndl, Gerhard, J Royal Netherlands Institute for Sea Research (NIOZ) Biologie des Organismes et Ecosystèmes Aquatiques (BOREA) Université de Caen Normandie (UNICAEN) Normandie Université (NU)-Normandie Université (NU)-Muséum national d'Histoire naturelle (MNHN)-Institut de Recherche pour le Développement (IRD)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Université des Antilles (UA) Marine Biological Laboratory (MBL) University of Chicago Universität Wien = University of Vienna 2011-01 https://hal.science/hal-01086716 https://hal.science/hal-01086716/document https://hal.science/hal-01086716/file/Agogueetal,%202011,%20MolEcol.pdf https://doi.org/10.1111/j.1365-294X.2010.04932.x en eng HAL CCSD Wiley info:eu-repo/semantics/altIdentifier/doi/10.1111/j.1365-294X.2010.04932.x hal-01086716 https://hal.science/hal-01086716 https://hal.science/hal-01086716/document https://hal.science/hal-01086716/file/Agogueetal,%202011,%20MolEcol.pdf doi:10.1111/j.1365-294X.2010.04932.x info:eu-repo/semantics/OpenAccess ISSN: 0962-1083 EISSN: 1365-294X Molecular Ecology https://hal.science/hal-01086716 Molecular Ecology, 2011, 20, http://onlinelibrary.wiley.com/journal/10.1111/%28ISSN%291365-294X. ⟨10.1111/j.1365-294X.2010.04932.x⟩ bacterial diversity massively parallel tag sequencing North Atlantic Ocean deep 17 water masses bacterial biogeography [SDE.BE]Environmental Sciences/Biodiversity and Ecology info:eu-repo/semantics/article Journal articles 2011 ftsorbonneuniv https://doi.org/10.1111/j.1365-294X.2010.04932.x10.1111/%28ISSN%291365-294X 2024-05-23T23:52:31Z International audience Bacterial assemblages from subsurface (100 m depth), meso- (200-1000 m depth) and bathy-pelagic (below 1000 m depth) zones at 10 stations along a North Atlantic Ocean transect from 60°N to 5°S were characterized using massively parallel pyrotag sequencing of the V6 region of the 16S rRNA gene (V6 pyrotags). In a dataset of more than 830,000 pyrotags we identified 10,780 OTUs of which 52% were singletons. The singletons accounted for less than 2% of the OTU abundance, while the 100 and 1,000 most abundant OTUs represented 80% and 96%, respectively, of all recovered OTUs. Non-metric Multi-Dimensional Scaling and Canonical Correspondence Analysis of all the OTUs excluding the singletons revealed a clear clustering of the bacterial communities according to the water masses. More than 80% of the 1,000 most abundant OTUs corresponded to Proteobacteria of which 55% were Alphaproteobacteria, mostly composed of the SAR11 cluster. Gammaproteobacteria increased with depth and included a relatively large number of OTUs belonging to Alteromonadales and Oceanospirillales. The bathypelagic zone showed higher taxonomic evenness than the overlying waters, albeit bacterial diversity was remarkably variable. Both abundant and low-abundance OTUs were responsible for the distinct bacterial communities characterizing the major deep-water masses. Taken together, our results reveal that deep-water masses act as bio-oceanographic islands for bacterioplankton leading to water mass-specific bacterial communities in the deep waters of the Atlantic. Article in Journal/Newspaper North Atlantic HAL Sorbonne Université Molecular Ecology 20 2 258 274 |
institution |
Open Polar |
collection |
HAL Sorbonne Université |
op_collection_id |
ftsorbonneuniv |
language |
English |
topic |
bacterial diversity massively parallel tag sequencing North Atlantic Ocean deep 17 water masses bacterial biogeography [SDE.BE]Environmental Sciences/Biodiversity and Ecology |
spellingShingle |
bacterial diversity massively parallel tag sequencing North Atlantic Ocean deep 17 water masses bacterial biogeography [SDE.BE]Environmental Sciences/Biodiversity and Ecology Agogué, Hélène Lamy, Dominique Neal, Phillip, R Sogin, Mitchell, S Herndl, Gerhard, J Water mass-specificity of bacterial communities in the North Atlantic revealed by massively parallel sequencing |
topic_facet |
bacterial diversity massively parallel tag sequencing North Atlantic Ocean deep 17 water masses bacterial biogeography [SDE.BE]Environmental Sciences/Biodiversity and Ecology |
description |
International audience Bacterial assemblages from subsurface (100 m depth), meso- (200-1000 m depth) and bathy-pelagic (below 1000 m depth) zones at 10 stations along a North Atlantic Ocean transect from 60°N to 5°S were characterized using massively parallel pyrotag sequencing of the V6 region of the 16S rRNA gene (V6 pyrotags). In a dataset of more than 830,000 pyrotags we identified 10,780 OTUs of which 52% were singletons. The singletons accounted for less than 2% of the OTU abundance, while the 100 and 1,000 most abundant OTUs represented 80% and 96%, respectively, of all recovered OTUs. Non-metric Multi-Dimensional Scaling and Canonical Correspondence Analysis of all the OTUs excluding the singletons revealed a clear clustering of the bacterial communities according to the water masses. More than 80% of the 1,000 most abundant OTUs corresponded to Proteobacteria of which 55% were Alphaproteobacteria, mostly composed of the SAR11 cluster. Gammaproteobacteria increased with depth and included a relatively large number of OTUs belonging to Alteromonadales and Oceanospirillales. The bathypelagic zone showed higher taxonomic evenness than the overlying waters, albeit bacterial diversity was remarkably variable. Both abundant and low-abundance OTUs were responsible for the distinct bacterial communities characterizing the major deep-water masses. Taken together, our results reveal that deep-water masses act as bio-oceanographic islands for bacterioplankton leading to water mass-specific bacterial communities in the deep waters of the Atlantic. |
author2 |
Royal Netherlands Institute for Sea Research (NIOZ) Biologie des Organismes et Ecosystèmes Aquatiques (BOREA) Université de Caen Normandie (UNICAEN) Normandie Université (NU)-Normandie Université (NU)-Muséum national d'Histoire naturelle (MNHN)-Institut de Recherche pour le Développement (IRD)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Université des Antilles (UA) Marine Biological Laboratory (MBL) University of Chicago Universität Wien = University of Vienna |
format |
Article in Journal/Newspaper |
author |
Agogué, Hélène Lamy, Dominique Neal, Phillip, R Sogin, Mitchell, S Herndl, Gerhard, J |
author_facet |
Agogué, Hélène Lamy, Dominique Neal, Phillip, R Sogin, Mitchell, S Herndl, Gerhard, J |
author_sort |
Agogué, Hélène |
title |
Water mass-specificity of bacterial communities in the North Atlantic revealed by massively parallel sequencing |
title_short |
Water mass-specificity of bacterial communities in the North Atlantic revealed by massively parallel sequencing |
title_full |
Water mass-specificity of bacterial communities in the North Atlantic revealed by massively parallel sequencing |
title_fullStr |
Water mass-specificity of bacterial communities in the North Atlantic revealed by massively parallel sequencing |
title_full_unstemmed |
Water mass-specificity of bacterial communities in the North Atlantic revealed by massively parallel sequencing |
title_sort |
water mass-specificity of bacterial communities in the north atlantic revealed by massively parallel sequencing |
publisher |
HAL CCSD |
publishDate |
2011 |
url |
https://hal.science/hal-01086716 https://hal.science/hal-01086716/document https://hal.science/hal-01086716/file/Agogueetal,%202011,%20MolEcol.pdf https://doi.org/10.1111/j.1365-294X.2010.04932.x |
genre |
North Atlantic |
genre_facet |
North Atlantic |
op_source |
ISSN: 0962-1083 EISSN: 1365-294X Molecular Ecology https://hal.science/hal-01086716 Molecular Ecology, 2011, 20, http://onlinelibrary.wiley.com/journal/10.1111/%28ISSN%291365-294X. ⟨10.1111/j.1365-294X.2010.04932.x⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1111/j.1365-294X.2010.04932.x hal-01086716 https://hal.science/hal-01086716 https://hal.science/hal-01086716/document https://hal.science/hal-01086716/file/Agogueetal,%202011,%20MolEcol.pdf doi:10.1111/j.1365-294X.2010.04932.x |
op_rights |
info:eu-repo/semantics/OpenAccess |
op_doi |
https://doi.org/10.1111/j.1365-294X.2010.04932.x10.1111/%28ISSN%291365-294X |
container_title |
Molecular Ecology |
container_volume |
20 |
container_issue |
2 |
container_start_page |
258 |
op_container_end_page |
274 |
_version_ |
1802008993119338496 |