Quenching correction for in vivo chlorophyll fluorescence acquired by autonomous platforms: a case study with instrumented elephant seals in the Kerguelen region (Southern Ocean)
International audience As the proxy for Chlorophyll a (Chl a) concentration, thousands of fluorescence profiles were measured by instrumented elephant seals in the Kerguelen region (Southern Ocean). For accurate retrieval of Chl a concentrations acquired by in vivo fluorometer, a two-step procedure...
Published in: | Limnology and Oceanography: Methods |
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Online Access: | https://hal.science/hal-00707656 https://doi.org/10.4319/lom.2012.10.483 |
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ftsorbonneuniv:oai:HAL:hal-00707656v1 2024-09-09T19:38:58+00:00 Quenching correction for in vivo chlorophyll fluorescence acquired by autonomous platforms: a case study with instrumented elephant seals in the Kerguelen region (Southern Ocean) Xing, Xiaogang Claustre, Hervé Blain, Stéphane d'Ortenzio, Fabrizio Antoine, David Ras, Josephine Guinet, Christophe Laboratoire d'océanographie de Villefranche (LOV) Observatoire océanologique de Villefranche-sur-mer (OOVM) Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Centre d'Études Biologiques de Chizé (CEBC) Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS) 2012 https://hal.science/hal-00707656 https://doi.org/10.4319/lom.2012.10.483 en eng HAL CCSD Association for the Sciences of Limnology and Oceanography info:eu-repo/semantics/altIdentifier/doi/10.4319/lom.2012.10.483 hal-00707656 https://hal.science/hal-00707656 doi:10.4319/lom.2012.10.483 ISSN: 1541-5856 Limnology and Oceanography: Methods https://hal.science/hal-00707656 Limnology and Oceanography: Methods, 2012, 10, pp.483-495. ⟨10.4319/lom.2012.10.483⟩ [SDE]Environmental Sciences info:eu-repo/semantics/article Journal articles 2012 ftsorbonneuniv https://doi.org/10.4319/lom.2012.10.483 2024-07-25T23:47:33Z International audience As the proxy for Chlorophyll a (Chl a) concentration, thousands of fluorescence profiles were measured by instrumented elephant seals in the Kerguelen region (Southern Ocean). For accurate retrieval of Chl a concentrations acquired by in vivo fluorometer, a two-step procedure is applied: 1) A predeployment intercalibration with accurate determination by high performance liquid chromatography (HPLC) analysis, which not only calibrates fluorescence in appropriate Chl a concentration units, but also strongly reduces variability between fluorometers, and 2) a profile-by-profile quenching correction analysis, which effectively eliminates the fluorescence quenching issue at surface around noon, and results in consistent profiles between day and night. The quenching correction is conducted through an extrapolation of the deep fluorescence value toward surface. As proved by a validation procedure in the Western Mediterranean Sea, the correction method is practical and relatively reliable when there is no credible reference, especially for deep mixed waters, as in the Southern Ocean. Even in the shallow mixed waters, the method is also effective in reducing the influence of quenching. Article in Journal/Newspaper Elephant Seals Southern Ocean HAL Sorbonne Université Southern Ocean Kerguelen Limnology and Oceanography: Methods 10 7 483 495 |
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Open Polar |
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HAL Sorbonne Université |
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ftsorbonneuniv |
language |
English |
topic |
[SDE]Environmental Sciences |
spellingShingle |
[SDE]Environmental Sciences Xing, Xiaogang Claustre, Hervé Blain, Stéphane d'Ortenzio, Fabrizio Antoine, David Ras, Josephine Guinet, Christophe Quenching correction for in vivo chlorophyll fluorescence acquired by autonomous platforms: a case study with instrumented elephant seals in the Kerguelen region (Southern Ocean) |
topic_facet |
[SDE]Environmental Sciences |
description |
International audience As the proxy for Chlorophyll a (Chl a) concentration, thousands of fluorescence profiles were measured by instrumented elephant seals in the Kerguelen region (Southern Ocean). For accurate retrieval of Chl a concentrations acquired by in vivo fluorometer, a two-step procedure is applied: 1) A predeployment intercalibration with accurate determination by high performance liquid chromatography (HPLC) analysis, which not only calibrates fluorescence in appropriate Chl a concentration units, but also strongly reduces variability between fluorometers, and 2) a profile-by-profile quenching correction analysis, which effectively eliminates the fluorescence quenching issue at surface around noon, and results in consistent profiles between day and night. The quenching correction is conducted through an extrapolation of the deep fluorescence value toward surface. As proved by a validation procedure in the Western Mediterranean Sea, the correction method is practical and relatively reliable when there is no credible reference, especially for deep mixed waters, as in the Southern Ocean. Even in the shallow mixed waters, the method is also effective in reducing the influence of quenching. |
author2 |
Laboratoire d'océanographie de Villefranche (LOV) Observatoire océanologique de Villefranche-sur-mer (OOVM) Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Centre d'Études Biologiques de Chizé (CEBC) Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS) |
format |
Article in Journal/Newspaper |
author |
Xing, Xiaogang Claustre, Hervé Blain, Stéphane d'Ortenzio, Fabrizio Antoine, David Ras, Josephine Guinet, Christophe |
author_facet |
Xing, Xiaogang Claustre, Hervé Blain, Stéphane d'Ortenzio, Fabrizio Antoine, David Ras, Josephine Guinet, Christophe |
author_sort |
Xing, Xiaogang |
title |
Quenching correction for in vivo chlorophyll fluorescence acquired by autonomous platforms: a case study with instrumented elephant seals in the Kerguelen region (Southern Ocean) |
title_short |
Quenching correction for in vivo chlorophyll fluorescence acquired by autonomous platforms: a case study with instrumented elephant seals in the Kerguelen region (Southern Ocean) |
title_full |
Quenching correction for in vivo chlorophyll fluorescence acquired by autonomous platforms: a case study with instrumented elephant seals in the Kerguelen region (Southern Ocean) |
title_fullStr |
Quenching correction for in vivo chlorophyll fluorescence acquired by autonomous platforms: a case study with instrumented elephant seals in the Kerguelen region (Southern Ocean) |
title_full_unstemmed |
Quenching correction for in vivo chlorophyll fluorescence acquired by autonomous platforms: a case study with instrumented elephant seals in the Kerguelen region (Southern Ocean) |
title_sort |
quenching correction for in vivo chlorophyll fluorescence acquired by autonomous platforms: a case study with instrumented elephant seals in the kerguelen region (southern ocean) |
publisher |
HAL CCSD |
publishDate |
2012 |
url |
https://hal.science/hal-00707656 https://doi.org/10.4319/lom.2012.10.483 |
geographic |
Southern Ocean Kerguelen |
geographic_facet |
Southern Ocean Kerguelen |
genre |
Elephant Seals Southern Ocean |
genre_facet |
Elephant Seals Southern Ocean |
op_source |
ISSN: 1541-5856 Limnology and Oceanography: Methods https://hal.science/hal-00707656 Limnology and Oceanography: Methods, 2012, 10, pp.483-495. ⟨10.4319/lom.2012.10.483⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.4319/lom.2012.10.483 hal-00707656 https://hal.science/hal-00707656 doi:10.4319/lom.2012.10.483 |
op_doi |
https://doi.org/10.4319/lom.2012.10.483 |
container_title |
Limnology and Oceanography: Methods |
container_volume |
10 |
container_issue |
7 |
container_start_page |
483 |
op_container_end_page |
495 |
_version_ |
1809908133773967360 |