Peroxiredoxin 6 gene: Anewphysiological and geneticindicator of multipleenvironmentalstressresponse in PacificoysterCrassostrea gigas
International audience Peroxiredoxin 6 is a 1-cysteine peroxiredoxin involved in antioxidant processes. We characterised the full-length cDNA and genomic sequence of the gene encoding peroxiredoxin 6 (CgPrx6) in the Pacificoyster, Crassostrea gigas. A phylogenetic analysis of Prx6 sequences showed t...
Published in: | Aquatic Toxicology |
---|---|
Main Authors: | , , |
Other Authors: | , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
HAL CCSD
2007
|
Subjects: | |
Online Access: | https://hal.univ-brest.fr/hal-00667042 https://doi.org/10.1016/j.aquatox.2007.06.017 |
id |
ftsorbonneuniv:oai:HAL:hal-00667042v1 |
---|---|
record_format |
openpolar |
spelling |
ftsorbonneuniv:oai:HAL:hal-00667042v1 2024-02-11T10:03:04+01:00 Peroxiredoxin 6 gene: Anewphysiological and geneticindicator of multipleenvironmentalstressresponse in PacificoysterCrassostrea gigas David, Elise Tanguy, Arnaud Moraga, Dario Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Adaptation et diversité en milieu marin (AD2M) Station biologique de Roscoff Roscoff (SBR) Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) 2007 https://hal.univ-brest.fr/hal-00667042 https://doi.org/10.1016/j.aquatox.2007.06.017 en eng HAL CCSD Elsevier info:eu-repo/semantics/altIdentifier/doi/10.1016/j.aquatox.2007.06.017 hal-00667042 https://hal.univ-brest.fr/hal-00667042 doi:10.1016/j.aquatox.2007.06.017 ISSN: 0166-445X Aquatic Toxicology https://hal.univ-brest.fr/hal-00667042 Aquatic Toxicology, 2007, 84 (3), pp.389-398. ⟨10.1016/j.aquatox.2007.06.017⟩ Crassostrea gigas Peroxiredoxin 6 Gene expression Polymorphism Environmentalstress [SDE]Environmental Sciences info:eu-repo/semantics/article Journal articles 2007 ftsorbonneuniv https://doi.org/10.1016/j.aquatox.2007.06.017 2024-01-23T23:36:17Z International audience Peroxiredoxin 6 is a 1-cysteine peroxiredoxin involved in antioxidant processes. We characterised the full-length cDNA and genomic sequence of the gene encoding peroxiredoxin 6 (CgPrx6) in the Pacificoyster, Crassostrea gigas. A phylogenetic analysis of Prx6 sequences showed that the CgPrx6 segregates between vertebrate and invertebrate groups. We analysed the expression of mRNA coding for CgPrx6 using RT-PCR in gills and digestive gland of oysters sampled in different contaminated and reference estuaries of the Atlantic French coast. We also studied CgPrx6 exon 6 polymorphism by PCR-SSCP in the same populations. Our results showed that CgPrx6 gene expression was highly regulated in the estuaries showing differential contamination levels, as expression increased with pollution level. Polymorphism analysis revealed no significant allelic frequency variations between populations. However, heterozygote deficiency seems to occur in the most contaminated estuaries, suggesting a potential selective effect of environmentalstress on heterozygote frequency. Finally, the use of CgPrx6 as a possible marker to monitor stress exposure in disturbed ecosystems is discussed. Article in Journal/Newspaper Crassostrea gigas HAL Sorbonne Université Aquatic Toxicology 84 3 389 398 |
institution |
Open Polar |
collection |
HAL Sorbonne Université |
op_collection_id |
ftsorbonneuniv |
language |
English |
topic |
Crassostrea gigas Peroxiredoxin 6 Gene expression Polymorphism Environmentalstress [SDE]Environmental Sciences |
spellingShingle |
Crassostrea gigas Peroxiredoxin 6 Gene expression Polymorphism Environmentalstress [SDE]Environmental Sciences David, Elise Tanguy, Arnaud Moraga, Dario Peroxiredoxin 6 gene: Anewphysiological and geneticindicator of multipleenvironmentalstressresponse in PacificoysterCrassostrea gigas |
topic_facet |
Crassostrea gigas Peroxiredoxin 6 Gene expression Polymorphism Environmentalstress [SDE]Environmental Sciences |
description |
International audience Peroxiredoxin 6 is a 1-cysteine peroxiredoxin involved in antioxidant processes. We characterised the full-length cDNA and genomic sequence of the gene encoding peroxiredoxin 6 (CgPrx6) in the Pacificoyster, Crassostrea gigas. A phylogenetic analysis of Prx6 sequences showed that the CgPrx6 segregates between vertebrate and invertebrate groups. We analysed the expression of mRNA coding for CgPrx6 using RT-PCR in gills and digestive gland of oysters sampled in different contaminated and reference estuaries of the Atlantic French coast. We also studied CgPrx6 exon 6 polymorphism by PCR-SSCP in the same populations. Our results showed that CgPrx6 gene expression was highly regulated in the estuaries showing differential contamination levels, as expression increased with pollution level. Polymorphism analysis revealed no significant allelic frequency variations between populations. However, heterozygote deficiency seems to occur in the most contaminated estuaries, suggesting a potential selective effect of environmentalstress on heterozygote frequency. Finally, the use of CgPrx6 as a possible marker to monitor stress exposure in disturbed ecosystems is discussed. |
author2 |
Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Adaptation et diversité en milieu marin (AD2M) Station biologique de Roscoff Roscoff (SBR) Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) |
format |
Article in Journal/Newspaper |
author |
David, Elise Tanguy, Arnaud Moraga, Dario |
author_facet |
David, Elise Tanguy, Arnaud Moraga, Dario |
author_sort |
David, Elise |
title |
Peroxiredoxin 6 gene: Anewphysiological and geneticindicator of multipleenvironmentalstressresponse in PacificoysterCrassostrea gigas |
title_short |
Peroxiredoxin 6 gene: Anewphysiological and geneticindicator of multipleenvironmentalstressresponse in PacificoysterCrassostrea gigas |
title_full |
Peroxiredoxin 6 gene: Anewphysiological and geneticindicator of multipleenvironmentalstressresponse in PacificoysterCrassostrea gigas |
title_fullStr |
Peroxiredoxin 6 gene: Anewphysiological and geneticindicator of multipleenvironmentalstressresponse in PacificoysterCrassostrea gigas |
title_full_unstemmed |
Peroxiredoxin 6 gene: Anewphysiological and geneticindicator of multipleenvironmentalstressresponse in PacificoysterCrassostrea gigas |
title_sort |
peroxiredoxin 6 gene: anewphysiological and geneticindicator of multipleenvironmentalstressresponse in pacificoystercrassostrea gigas |
publisher |
HAL CCSD |
publishDate |
2007 |
url |
https://hal.univ-brest.fr/hal-00667042 https://doi.org/10.1016/j.aquatox.2007.06.017 |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_source |
ISSN: 0166-445X Aquatic Toxicology https://hal.univ-brest.fr/hal-00667042 Aquatic Toxicology, 2007, 84 (3), pp.389-398. ⟨10.1016/j.aquatox.2007.06.017⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.aquatox.2007.06.017 hal-00667042 https://hal.univ-brest.fr/hal-00667042 doi:10.1016/j.aquatox.2007.06.017 |
op_doi |
https://doi.org/10.1016/j.aquatox.2007.06.017 |
container_title |
Aquatic Toxicology |
container_volume |
84 |
container_issue |
3 |
container_start_page |
389 |
op_container_end_page |
398 |
_version_ |
1790599215399829504 |