Genetically based resistance to summer mortality in the Pacific oyster (Crassostrea gigas) and its relationship with physiological, immunological characteristics and infection processes
International audience Summer mortality of Pacific oysters is known in several countries. However no specific pathogen has been systematically associated with this phenomenon. A complex combination of environmental and biological parameters has been suggested as the cause and is now starting to be i...
Published in: | Aquaculture |
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Main Authors: | , , , , , , , , , , , , , , , , , , , , , , , |
Other Authors: | , , , , , , , , , , , , , , , , , , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
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HAL CCSD
2007
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Subjects: | |
Online Access: | https://hal.science/hal-00473457 https://hal.science/hal-00473457/document https://hal.science/hal-00473457/file/Samain_et_al_2007_Aquaculture_268_227-243_-auteurs.pdf https://doi.org/10.1016/j.aquaculture.2007.04.044 |
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institution |
Open Polar |
collection |
HAL Sorbonne Université |
op_collection_id |
ftsorbonneuniv |
language |
English |
topic |
Summer mortality Pacific Oyster Crassostrea gigas Resistant oyster Genetic selection Reproduction Hemocytes Stress Temperature ROS [SDV.BA]Life Sciences [q-bio]/Animal biology |
spellingShingle |
Summer mortality Pacific Oyster Crassostrea gigas Resistant oyster Genetic selection Reproduction Hemocytes Stress Temperature ROS [SDV.BA]Life Sciences [q-bio]/Animal biology Samain, Jean-François Degremont, Lionel Soletchnik, Patrick Haure, Joel Bédier, Edouard Ropert, Michel Moal, Jeanne Huvet, Arnaud Bacca, Hélène van Wormhoudt, Alain Delaporte, Maryse Costil, K. Pouvreau, Stéphane Lambert, Christophe Boulo, Viviane Soudant, Philippe Nicolas, Jean-Louis Le Roux, Frédérique Renault, Tristan Gaganaire, Béatrice Geret, H. Boutet, Isabelle Burgeot, Thierry Boudry, Pierre Genetically based resistance to summer mortality in the Pacific oyster (Crassostrea gigas) and its relationship with physiological, immunological characteristics and infection processes |
topic_facet |
Summer mortality Pacific Oyster Crassostrea gigas Resistant oyster Genetic selection Reproduction Hemocytes Stress Temperature ROS [SDV.BA]Life Sciences [q-bio]/Animal biology |
description |
International audience Summer mortality of Pacific oysters is known in several countries. However no specific pathogen has been systematically associated with this phenomenon. A complex combination of environmental and biological parameters has been suggested as the cause and is now starting to be identified. A high genetic basis was found for survival in oysters when a first generation (G1) was tested in three sites during summer. This paper presents a synthesis on physiological characteristics of two selected groups (‘R' and ‘S', from families selected for resistance and susceptibility to summer mortality respectively), of the second and third generations. R and S showed improvement or reduction of survival compared with the control in both field and laboratory trials confirming the high heritability of survival of juveniles <1 year old. Interestingly, no correlation was observed between growth and survival. Comparison between the two selected groups showed that S oysters invested more energy in reproduction and stayed a longer time without spawning than R oysters which had high synchronous spawning. This was mainly shown with high rather than low dietary rations (respectively 12% and 4% DW algae/DW oyster) in a controlled experiment. Moreover, early partial spawning was detected in S oysters and not R ones in the high dietary ration. S showed a higher respiration rate and an earlier decrease in absorption efficiency than R during gametogenesis, but they were not significantly different in glycogen or ATP utilisation. Two months before a mortality episode, hemocytes from S oysters had a higher adhesive capacity than R hemocytes and significantly higher reactive oxygen species production capacity. One month before mortality, S oysters had the highest hyalinocyte concentration and their expression of genes coding for glucose metabolism enzymes (Hexokinase, GS, PGM, PEPCK) was significantly lower in the labial palps. After a thermal increase from 13 °C to 19 °C, during 8 days in normoxia, S oysters showed a ... |
author2 |
Physiologie et Ecophysiologie des Mollusques Marins (PE2M) Université de Caen Normandie (UNICAEN) Normandie Université (NU)-Normandie Université (NU)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Centre National de la Recherche Scientifique (CNRS) Unité Amélioration génétique, Santé animale et Environnement (AGSAE) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) Laboratoire Environnement Ressources des Pertuis Charentais (LERPC) Unité Littoral (LITTORAL) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) Laboratoire Environnement Ressources Morbihan Pays de Loire (LERMPL) Laboratoire Environnement Ressources de Normandie (LERN) Station de Biologie Marine de Concarneau Direction générale déléguée à la Recherche, à l’Expertise, à la Valorisation et à l’Enseignement-Formation (DGD.REVE) Muséum national d'Histoire naturelle (MNHN)-Muséum national d'Histoire naturelle (MNHN) Biologie des organismes marins et écosystèmes (BOME) Muséum national d'Histoire naturelle (MNHN)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS) Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Unité Lagons, Ecosystèmes et Aquaculture Durable en Nouvelle-Calédonie (LEADNC) Génome, populations, interactions, adaptation (GPIA) Université Montpellier 2 - Sciences et Techniques (UM2)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Centre National de la Recherche Scientifique (CNRS) Université européenne de Bretagne - European University of Brittany (UEB) Unité Biogéochimie et Ecotoxicologie (BE) Station biologique de Roscoff Roscoff (SBR) Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS) Régions of Basse-Normandie, Bretagne, Pays de la Loire, Poitou-Charentes; Conseil Général du Calvados; National Morest project funded by Ifremer; |
format |
Article in Journal/Newspaper |
author |
Samain, Jean-François Degremont, Lionel Soletchnik, Patrick Haure, Joel Bédier, Edouard Ropert, Michel Moal, Jeanne Huvet, Arnaud Bacca, Hélène van Wormhoudt, Alain Delaporte, Maryse Costil, K. Pouvreau, Stéphane Lambert, Christophe Boulo, Viviane Soudant, Philippe Nicolas, Jean-Louis Le Roux, Frédérique Renault, Tristan Gaganaire, Béatrice Geret, H. Boutet, Isabelle Burgeot, Thierry Boudry, Pierre |
author_facet |
Samain, Jean-François Degremont, Lionel Soletchnik, Patrick Haure, Joel Bédier, Edouard Ropert, Michel Moal, Jeanne Huvet, Arnaud Bacca, Hélène van Wormhoudt, Alain Delaporte, Maryse Costil, K. Pouvreau, Stéphane Lambert, Christophe Boulo, Viviane Soudant, Philippe Nicolas, Jean-Louis Le Roux, Frédérique Renault, Tristan Gaganaire, Béatrice Geret, H. Boutet, Isabelle Burgeot, Thierry Boudry, Pierre |
author_sort |
Samain, Jean-François |
title |
Genetically based resistance to summer mortality in the Pacific oyster (Crassostrea gigas) and its relationship with physiological, immunological characteristics and infection processes |
title_short |
Genetically based resistance to summer mortality in the Pacific oyster (Crassostrea gigas) and its relationship with physiological, immunological characteristics and infection processes |
title_full |
Genetically based resistance to summer mortality in the Pacific oyster (Crassostrea gigas) and its relationship with physiological, immunological characteristics and infection processes |
title_fullStr |
Genetically based resistance to summer mortality in the Pacific oyster (Crassostrea gigas) and its relationship with physiological, immunological characteristics and infection processes |
title_full_unstemmed |
Genetically based resistance to summer mortality in the Pacific oyster (Crassostrea gigas) and its relationship with physiological, immunological characteristics and infection processes |
title_sort |
genetically based resistance to summer mortality in the pacific oyster (crassostrea gigas) and its relationship with physiological, immunological characteristics and infection processes |
publisher |
HAL CCSD |
publishDate |
2007 |
url |
https://hal.science/hal-00473457 https://hal.science/hal-00473457/document https://hal.science/hal-00473457/file/Samain_et_al_2007_Aquaculture_268_227-243_-auteurs.pdf https://doi.org/10.1016/j.aquaculture.2007.04.044 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
ISSN: 0044-8486 EISSN: 1873-5622 Aquaculture https://hal.science/hal-00473457 Aquaculture, 2007, 268, pp.227-243. ⟨10.1016/j.aquaculture.2007.04.044⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.aquaculture.2007.04.044 hal-00473457 https://hal.science/hal-00473457 https://hal.science/hal-00473457/document https://hal.science/hal-00473457/file/Samain_et_al_2007_Aquaculture_268_227-243_-auteurs.pdf doi:10.1016/j.aquaculture.2007.04.044 |
op_rights |
info:eu-repo/semantics/OpenAccess |
op_doi |
https://doi.org/10.1016/j.aquaculture.2007.04.044 |
container_title |
Aquaculture |
container_volume |
268 |
container_issue |
1-4 |
container_start_page |
227 |
op_container_end_page |
243 |
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1798844867507388416 |
spelling |
ftsorbonneuniv:oai:HAL:hal-00473457v1 2024-05-12T08:02:43+00:00 Genetically based resistance to summer mortality in the Pacific oyster (Crassostrea gigas) and its relationship with physiological, immunological characteristics and infection processes Samain, Jean-François Degremont, Lionel Soletchnik, Patrick Haure, Joel Bédier, Edouard Ropert, Michel Moal, Jeanne Huvet, Arnaud Bacca, Hélène van Wormhoudt, Alain Delaporte, Maryse Costil, K. Pouvreau, Stéphane Lambert, Christophe Boulo, Viviane Soudant, Philippe Nicolas, Jean-Louis Le Roux, Frédérique Renault, Tristan Gaganaire, Béatrice Geret, H. Boutet, Isabelle Burgeot, Thierry Boudry, Pierre Physiologie et Ecophysiologie des Mollusques Marins (PE2M) Université de Caen Normandie (UNICAEN) Normandie Université (NU)-Normandie Université (NU)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Centre National de la Recherche Scientifique (CNRS) Unité Amélioration génétique, Santé animale et Environnement (AGSAE) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) Laboratoire Environnement Ressources des Pertuis Charentais (LERPC) Unité Littoral (LITTORAL) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) Laboratoire Environnement Ressources Morbihan Pays de Loire (LERMPL) Laboratoire Environnement Ressources de Normandie (LERN) Station de Biologie Marine de Concarneau Direction générale déléguée à la Recherche, à l’Expertise, à la Valorisation et à l’Enseignement-Formation (DGD.REVE) Muséum national d'Histoire naturelle (MNHN)-Muséum national d'Histoire naturelle (MNHN) Biologie des organismes marins et écosystèmes (BOME) Muséum national d'Histoire naturelle (MNHN)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS) Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Unité Lagons, Ecosystèmes et Aquaculture Durable en Nouvelle-Calédonie (LEADNC) Génome, populations, interactions, adaptation (GPIA) Université Montpellier 2 - Sciences et Techniques (UM2)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Centre National de la Recherche Scientifique (CNRS) Université européenne de Bretagne - European University of Brittany (UEB) Unité Biogéochimie et Ecotoxicologie (BE) Station biologique de Roscoff Roscoff (SBR) Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS) Régions of Basse-Normandie, Bretagne, Pays de la Loire, Poitou-Charentes; Conseil Général du Calvados; National Morest project funded by Ifremer; 2007 https://hal.science/hal-00473457 https://hal.science/hal-00473457/document https://hal.science/hal-00473457/file/Samain_et_al_2007_Aquaculture_268_227-243_-auteurs.pdf https://doi.org/10.1016/j.aquaculture.2007.04.044 en eng HAL CCSD Elsevier info:eu-repo/semantics/altIdentifier/doi/10.1016/j.aquaculture.2007.04.044 hal-00473457 https://hal.science/hal-00473457 https://hal.science/hal-00473457/document https://hal.science/hal-00473457/file/Samain_et_al_2007_Aquaculture_268_227-243_-auteurs.pdf doi:10.1016/j.aquaculture.2007.04.044 info:eu-repo/semantics/OpenAccess ISSN: 0044-8486 EISSN: 1873-5622 Aquaculture https://hal.science/hal-00473457 Aquaculture, 2007, 268, pp.227-243. ⟨10.1016/j.aquaculture.2007.04.044⟩ Summer mortality Pacific Oyster Crassostrea gigas Resistant oyster Genetic selection Reproduction Hemocytes Stress Temperature ROS [SDV.BA]Life Sciences [q-bio]/Animal biology info:eu-repo/semantics/article Journal articles 2007 ftsorbonneuniv https://doi.org/10.1016/j.aquaculture.2007.04.044 2024-04-18T03:20:14Z International audience Summer mortality of Pacific oysters is known in several countries. However no specific pathogen has been systematically associated with this phenomenon. A complex combination of environmental and biological parameters has been suggested as the cause and is now starting to be identified. A high genetic basis was found for survival in oysters when a first generation (G1) was tested in three sites during summer. This paper presents a synthesis on physiological characteristics of two selected groups (‘R' and ‘S', from families selected for resistance and susceptibility to summer mortality respectively), of the second and third generations. R and S showed improvement or reduction of survival compared with the control in both field and laboratory trials confirming the high heritability of survival of juveniles <1 year old. Interestingly, no correlation was observed between growth and survival. Comparison between the two selected groups showed that S oysters invested more energy in reproduction and stayed a longer time without spawning than R oysters which had high synchronous spawning. This was mainly shown with high rather than low dietary rations (respectively 12% and 4% DW algae/DW oyster) in a controlled experiment. Moreover, early partial spawning was detected in S oysters and not R ones in the high dietary ration. S showed a higher respiration rate and an earlier decrease in absorption efficiency than R during gametogenesis, but they were not significantly different in glycogen or ATP utilisation. Two months before a mortality episode, hemocytes from S oysters had a higher adhesive capacity than R hemocytes and significantly higher reactive oxygen species production capacity. One month before mortality, S oysters had the highest hyalinocyte concentration and their expression of genes coding for glucose metabolism enzymes (Hexokinase, GS, PGM, PEPCK) was significantly lower in the labial palps. After a thermal increase from 13 °C to 19 °C, during 8 days in normoxia, S oysters showed a ... Article in Journal/Newspaper Crassostrea gigas Pacific oyster HAL Sorbonne Université Pacific Aquaculture 268 1-4 227 243 |