Proteomic characterization of Pseudogymnoascus spp. isolates from polar and temperate regions
Proteomics has been used extensively in the field of mycology, mainly in trying to understand the complex network of protein-protein interactions that has been implicated in the molecular functions of fungi. It is also a useful tool to compare metabolic differences within a genus. Species of Pseudog...
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ftsmithonianinsp:oai:figshare.com:article/25412307 2024-04-14T08:04:34+00:00 Proteomic characterization of Pseudogymnoascus spp. isolates from polar and temperate regions Nurlizah Abu Bakar Benjamin Lau Yii Chung Jerzy Smykla Saiful Anuar Karsani Siti Aisyah Alias 2024-03-14T20:40:28Z https://doi.org/10.6084/m9.figshare.25412307.v1 unknown https://figshare.com/articles/dataset/Proteomic_characterization_of_i_Pseudogymnoascus_i_spp_isolates_from_polar_and_temperate_regions/25412307 doi:10.6084/m9.figshare.25412307.v1 CC BY 4.0 Biophysics Biochemistry Cell Biology Ecology Virology Environmental Sciences not elsewhere classified Biological Sciences not elsewhere classified Chemical Sciences not elsewhere classified Physical Sciences not elsewhere classified Biogeographic regions fungal proteomics soil microfungi tandem mass spectrometry Dataset 2024 ftsmithonianinsp https://doi.org/10.6084/m9.figshare.25412307.v1 2024-03-18T19:39:50Z Proteomics has been used extensively in the field of mycology, mainly in trying to understand the complex network of protein-protein interactions that has been implicated in the molecular functions of fungi. It is also a useful tool to compare metabolic differences within a genus. Species of Pseudogymnoascus , a genus under the phyla Ascomycota, have been shown to play an important role in the soil environment. They have been found in both polar and temperate regions and are a known producer of many extracellular hydrolases that contribute to soil decomposition. Despite the apparent importance of Pseudogymnoascus spp. in the soil ecosystem, investigations into their molecular functions are still very limited. In the present study, proteomic characterization of six Pseudogymnoascus spp. isolated from three biogeographic regions (the Arctic, Antarctic, and temperate regions) was carried out using tandem mass spectrometry. Prior to proteomic analysis, the optimization for protein extraction was carried out. Trichloroacetic acid‑acetone‑phenol was found to be the best extraction method to be used for proteomic profiling of Pseudogymnoascus spp. The proteomic analysis identified 2003 proteins that were successfully mapped to the UniProtKB database. The identified proteins were clustered according to their biological processes and molecular functions. The shared proteins found in all Pseudogymnoascus spp. (1201 proteins) showed a significantly close relationship in their basic cellular functions, despite differences in morphological structures. Analysis of Pseudogymnoascus spp. proteome also identified proteins that were unique to each region. However, a high number of these proteins belonged to protein families of similar molecular functions, namely, transferases and hydrolases. Our proteomic data can be used as a reference for Pseudogymnoascus spp. across different global regions and a foundation for future soil ecosystem function research. Dataset Antarc* Antarctic Arctic Smithsonian Institution: Figshare Arctic Antarctic |
institution |
Open Polar |
collection |
Smithsonian Institution: Figshare |
op_collection_id |
ftsmithonianinsp |
language |
unknown |
topic |
Biophysics Biochemistry Cell Biology Ecology Virology Environmental Sciences not elsewhere classified Biological Sciences not elsewhere classified Chemical Sciences not elsewhere classified Physical Sciences not elsewhere classified Biogeographic regions fungal proteomics soil microfungi tandem mass spectrometry |
spellingShingle |
Biophysics Biochemistry Cell Biology Ecology Virology Environmental Sciences not elsewhere classified Biological Sciences not elsewhere classified Chemical Sciences not elsewhere classified Physical Sciences not elsewhere classified Biogeographic regions fungal proteomics soil microfungi tandem mass spectrometry Nurlizah Abu Bakar Benjamin Lau Yii Chung Jerzy Smykla Saiful Anuar Karsani Siti Aisyah Alias Proteomic characterization of Pseudogymnoascus spp. isolates from polar and temperate regions |
topic_facet |
Biophysics Biochemistry Cell Biology Ecology Virology Environmental Sciences not elsewhere classified Biological Sciences not elsewhere classified Chemical Sciences not elsewhere classified Physical Sciences not elsewhere classified Biogeographic regions fungal proteomics soil microfungi tandem mass spectrometry |
description |
Proteomics has been used extensively in the field of mycology, mainly in trying to understand the complex network of protein-protein interactions that has been implicated in the molecular functions of fungi. It is also a useful tool to compare metabolic differences within a genus. Species of Pseudogymnoascus , a genus under the phyla Ascomycota, have been shown to play an important role in the soil environment. They have been found in both polar and temperate regions and are a known producer of many extracellular hydrolases that contribute to soil decomposition. Despite the apparent importance of Pseudogymnoascus spp. in the soil ecosystem, investigations into their molecular functions are still very limited. In the present study, proteomic characterization of six Pseudogymnoascus spp. isolated from three biogeographic regions (the Arctic, Antarctic, and temperate regions) was carried out using tandem mass spectrometry. Prior to proteomic analysis, the optimization for protein extraction was carried out. Trichloroacetic acid‑acetone‑phenol was found to be the best extraction method to be used for proteomic profiling of Pseudogymnoascus spp. The proteomic analysis identified 2003 proteins that were successfully mapped to the UniProtKB database. The identified proteins were clustered according to their biological processes and molecular functions. The shared proteins found in all Pseudogymnoascus spp. (1201 proteins) showed a significantly close relationship in their basic cellular functions, despite differences in morphological structures. Analysis of Pseudogymnoascus spp. proteome also identified proteins that were unique to each region. However, a high number of these proteins belonged to protein families of similar molecular functions, namely, transferases and hydrolases. Our proteomic data can be used as a reference for Pseudogymnoascus spp. across different global regions and a foundation for future soil ecosystem function research. |
format |
Dataset |
author |
Nurlizah Abu Bakar Benjamin Lau Yii Chung Jerzy Smykla Saiful Anuar Karsani Siti Aisyah Alias |
author_facet |
Nurlizah Abu Bakar Benjamin Lau Yii Chung Jerzy Smykla Saiful Anuar Karsani Siti Aisyah Alias |
author_sort |
Nurlizah Abu Bakar |
title |
Proteomic characterization of Pseudogymnoascus spp. isolates from polar and temperate regions |
title_short |
Proteomic characterization of Pseudogymnoascus spp. isolates from polar and temperate regions |
title_full |
Proteomic characterization of Pseudogymnoascus spp. isolates from polar and temperate regions |
title_fullStr |
Proteomic characterization of Pseudogymnoascus spp. isolates from polar and temperate regions |
title_full_unstemmed |
Proteomic characterization of Pseudogymnoascus spp. isolates from polar and temperate regions |
title_sort |
proteomic characterization of pseudogymnoascus spp. isolates from polar and temperate regions |
publishDate |
2024 |
url |
https://doi.org/10.6084/m9.figshare.25412307.v1 |
geographic |
Arctic Antarctic |
geographic_facet |
Arctic Antarctic |
genre |
Antarc* Antarctic Arctic |
genre_facet |
Antarc* Antarctic Arctic |
op_relation |
https://figshare.com/articles/dataset/Proteomic_characterization_of_i_Pseudogymnoascus_i_spp_isolates_from_polar_and_temperate_regions/25412307 doi:10.6084/m9.figshare.25412307.v1 |
op_rights |
CC BY 4.0 |
op_doi |
https://doi.org/10.6084/m9.figshare.25412307.v1 |
_version_ |
1796301198488240128 |