Maximum likelihood tree based on the partial 16S rRNA gene sequences of the 11 Pseudomonas strains and closely related validly published strains of the Pseudomonas genus obtained from NCBI database.

• Main Authors: Srijana Mukhia (9025058), Anil Kumar (105720), Poonam Kumari (6175139), Rakshak Kumar (125841), Sanjay Kumar (8853)
• Format: Still Image
• Language: unknown
• Published: 2022
• Subjects: Microbiology; Genetics; Biotechnology; Evolutionary Biology; Ecology; Marine Biology; Inorganic Chemistry; Environmental Sciences not elsewhere classified; Biological Sciences not elsewhere classified; Chemical Sciences not elsewhere classified; unique adaptive features; mesophilic control strain; extreme environmental conditions; 96 hours compared; 73 %) compared; showed taxonomic ambiguity; highest ani value; high ultraviolet radiations; div >< p; pseudomonas antarctica </; obtained eleven strains; pseudomonas </; p </; antarctica </; taxonomic assignment; dddh value; c radiations; fluorescens </; tolerance abilities; supraglacial site; supraglacial ice; sikkim himalaya; representative strains; radiation resistance; polyadaptational attributes; physiological findings; pamc 27494; microorganisms inhabiting; frequent freeze; four strains; diverse genera; current study; correct taxonomy; biotechnologically significant; based assays; associated genes; adaptational strategies; active enzymes; 65 %); 05 could; Antarc*; Antarctica
• Online Access: https://doi.org/10.1371/journal.pone.0261178.g002

The 11 Pseudomonas strains show clustering with both P . fluorescens and P . antarctica . E . coli K12 MG1655 was used as the outgroup organism. The tree was constructed using MEGA X software based on the Kimura 2-parameter model with 1000 bootstrap replications, and the scale bar corresponds to the average number of nucleotide substitutions per site. Accession numbers are given in parenthesis.