Data_Sheet_1_Size-Fractionated Microbiome Structure in Subarctic Rivers and a Coastal Plume Across DOC and Salinity Gradients.ZIP
Little is known about the microbial diversity of rivers that flow across the changing subarctic landscape. Using amplicon sequencing (rRNA and rRNA genes) combined with HPLC pigment analysis and physicochemical measurements, we investigated the diversity of two size fractions of planktonic Bacteria,...
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ftsmithonian:oai:figshare.com:article/17714504 2023-05-15T16:23:09+02:00 Data_Sheet_1_Size-Fractionated Microbiome Structure in Subarctic Rivers and a Coastal Plume Across DOC and Salinity Gradients.ZIP Marie-Amélie Blais (8022281) Alex Matveev (4623916) Connie Lovejoy (133554) Warwick F. Vincent (6957164) 2022-01-03T05:28:17Z https://doi.org/10.3389/fmicb.2021.760282.s001 unknown https://figshare.com/articles/dataset/Data_Sheet_1_Size-Fractionated_Microbiome_Structure_in_Subarctic_Rivers_and_a_Coastal_Plume_Across_DOC_and_Salinity_Gradients_ZIP/17714504 doi:10.3389/fmicb.2021.760282.s001 CC BY 4.0 CC-BY Microbiology Microbial Genetics Microbial Ecology Mycology bacteria microbial eukaryotes permafrost river microbiomes climate change salinity dissolved organic carbon northern ecosystems Dataset 2022 ftsmithonian https://doi.org/10.3389/fmicb.2021.760282.s001 2022-01-06T11:15:08Z Little is known about the microbial diversity of rivers that flow across the changing subarctic landscape. Using amplicon sequencing (rRNA and rRNA genes) combined with HPLC pigment analysis and physicochemical measurements, we investigated the diversity of two size fractions of planktonic Bacteria, Archaea and microbial eukaryotes along environmental gradients in the Great Whale River (GWR), Canada. This large subarctic river drains an extensive watershed that includes areas of thawing permafrost, and discharges into southeastern Hudson Bay as an extensive plume that gradually mixes with the coastal marine waters. The microbial communities differed by size-fraction (separated with a 3-μm filter), and clustered into three distinct environmental groups: (1) the GWR sites throughout a 150-km sampling transect; (2) the GWR plume in Hudson Bay; and (3) small rivers that flow through degraded permafrost landscapes. There was a downstream increase in taxonomic richness along the GWR, suggesting that sub-catchment inputs influence microbial community structure in the absence of sharp environmental gradients. Microbial community structure shifted across the salinity gradient within the plume, with changes in taxonomic composition and diversity. Rivers flowing through degraded permafrost had distinct physicochemical and microbiome characteristics, with allochthonous dissolved organic carbon explaining part of the variation in community structure. Finally, our analyses of the core microbiome indicated that while a substantial part of all communities consisted of generalists, most taxa had a more limited environmental range and may therefore be sensitive to ongoing change. Dataset Great Whale River Hudson Bay permafrost Subarctic Unknown Hudson Bay Canada Hudson |
institution |
Open Polar |
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Unknown |
op_collection_id |
ftsmithonian |
language |
unknown |
topic |
Microbiology Microbial Genetics Microbial Ecology Mycology bacteria microbial eukaryotes permafrost river microbiomes climate change salinity dissolved organic carbon northern ecosystems |
spellingShingle |
Microbiology Microbial Genetics Microbial Ecology Mycology bacteria microbial eukaryotes permafrost river microbiomes climate change salinity dissolved organic carbon northern ecosystems Marie-Amélie Blais (8022281) Alex Matveev (4623916) Connie Lovejoy (133554) Warwick F. Vincent (6957164) Data_Sheet_1_Size-Fractionated Microbiome Structure in Subarctic Rivers and a Coastal Plume Across DOC and Salinity Gradients.ZIP |
topic_facet |
Microbiology Microbial Genetics Microbial Ecology Mycology bacteria microbial eukaryotes permafrost river microbiomes climate change salinity dissolved organic carbon northern ecosystems |
description |
Little is known about the microbial diversity of rivers that flow across the changing subarctic landscape. Using amplicon sequencing (rRNA and rRNA genes) combined with HPLC pigment analysis and physicochemical measurements, we investigated the diversity of two size fractions of planktonic Bacteria, Archaea and microbial eukaryotes along environmental gradients in the Great Whale River (GWR), Canada. This large subarctic river drains an extensive watershed that includes areas of thawing permafrost, and discharges into southeastern Hudson Bay as an extensive plume that gradually mixes with the coastal marine waters. The microbial communities differed by size-fraction (separated with a 3-μm filter), and clustered into three distinct environmental groups: (1) the GWR sites throughout a 150-km sampling transect; (2) the GWR plume in Hudson Bay; and (3) small rivers that flow through degraded permafrost landscapes. There was a downstream increase in taxonomic richness along the GWR, suggesting that sub-catchment inputs influence microbial community structure in the absence of sharp environmental gradients. Microbial community structure shifted across the salinity gradient within the plume, with changes in taxonomic composition and diversity. Rivers flowing through degraded permafrost had distinct physicochemical and microbiome characteristics, with allochthonous dissolved organic carbon explaining part of the variation in community structure. Finally, our analyses of the core microbiome indicated that while a substantial part of all communities consisted of generalists, most taxa had a more limited environmental range and may therefore be sensitive to ongoing change. |
format |
Dataset |
author |
Marie-Amélie Blais (8022281) Alex Matveev (4623916) Connie Lovejoy (133554) Warwick F. Vincent (6957164) |
author_facet |
Marie-Amélie Blais (8022281) Alex Matveev (4623916) Connie Lovejoy (133554) Warwick F. Vincent (6957164) |
author_sort |
Marie-Amélie Blais (8022281) |
title |
Data_Sheet_1_Size-Fractionated Microbiome Structure in Subarctic Rivers and a Coastal Plume Across DOC and Salinity Gradients.ZIP |
title_short |
Data_Sheet_1_Size-Fractionated Microbiome Structure in Subarctic Rivers and a Coastal Plume Across DOC and Salinity Gradients.ZIP |
title_full |
Data_Sheet_1_Size-Fractionated Microbiome Structure in Subarctic Rivers and a Coastal Plume Across DOC and Salinity Gradients.ZIP |
title_fullStr |
Data_Sheet_1_Size-Fractionated Microbiome Structure in Subarctic Rivers and a Coastal Plume Across DOC and Salinity Gradients.ZIP |
title_full_unstemmed |
Data_Sheet_1_Size-Fractionated Microbiome Structure in Subarctic Rivers and a Coastal Plume Across DOC and Salinity Gradients.ZIP |
title_sort |
data_sheet_1_size-fractionated microbiome structure in subarctic rivers and a coastal plume across doc and salinity gradients.zip |
publishDate |
2022 |
url |
https://doi.org/10.3389/fmicb.2021.760282.s001 |
geographic |
Hudson Bay Canada Hudson |
geographic_facet |
Hudson Bay Canada Hudson |
genre |
Great Whale River Hudson Bay permafrost Subarctic |
genre_facet |
Great Whale River Hudson Bay permafrost Subarctic |
op_relation |
https://figshare.com/articles/dataset/Data_Sheet_1_Size-Fractionated_Microbiome_Structure_in_Subarctic_Rivers_and_a_Coastal_Plume_Across_DOC_and_Salinity_Gradients_ZIP/17714504 doi:10.3389/fmicb.2021.760282.s001 |
op_rights |
CC BY 4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.3389/fmicb.2021.760282.s001 |
_version_ |
1766011355615199232 |