Image_2_Macrophage Heterogeneity in the Intestinal Cells of Salmon: Hints From Transcriptomic and Imaging Data.jpeg
The intestine has many types of cells that are present mostly in the epithelium and lamina propria. The importance of the intestinal cells for the mammalian mucosal immune system is well-established. However, there is no in-depth information about many of the intestinal cells in teleosts. In our pre...
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ftsmithonian:oai:figshare.com:article/17426294 2023-05-15T15:32:50+02:00 Image_2_Macrophage Heterogeneity in the Intestinal Cells of Salmon: Hints From Transcriptomic and Imaging Data.jpeg Youngjin Park (195938) Qirui Zhang (555365) Jorge M. O. Fernandes (7381673) Geert F. Wiegertjes (6790895) Viswanath Kiron (252416) 2021-12-23T04:44:02Z https://doi.org/10.3389/fimmu.2021.798156.s003 unknown https://figshare.com/articles/figure/Image_2_Macrophage_Heterogeneity_in_the_Intestinal_Cells_of_Salmon_Hints_From_Transcriptomic_and_Imaging_Data_jpeg/17426294 doi:10.3389/fimmu.2021.798156.s003 CC BY 4.0 CC-BY Immunology Applied Immunology (incl. Antibody Engineering Xenotransplantation and T-cell Therapies) Autoimmunity Cellular Immunology Humoural Immunology and Immunochemistry Immunogenetics (incl. Genetic Immunology) Innate Immunity Transplantation Immunology Tumour Immunology Immunology not elsewhere classified Genetic Immunology Animal Immunology Veterinary Immunology adherent cells intestinal cells macrophages RNA-seq miRNAs Atlantic salmon Image Figure 2021 ftsmithonian https://doi.org/10.3389/fimmu.2021.798156.s003 2022-01-06T11:42:39Z The intestine has many types of cells that are present mostly in the epithelium and lamina propria. The importance of the intestinal cells for the mammalian mucosal immune system is well-established. However, there is no in-depth information about many of the intestinal cells in teleosts. In our previous study, we reported that adherent intestinal cells (AIC) predominantly express macrophage-related genes. To gather further evidence that AIC include macrophage-like cells, we compared their phagocytic activity and morphology with those of adherent head kidney cells (AKC), previously characterized as macrophage-like cells. We also compared equally abundant as well as differentially expressed mRNAs and miRNAs between AIC and AKC. AIC had lower phagocytic activity and were larger and more circular than macrophage-like AKC. RNA-Seq data revealed that there were 18309 mRNAs, with 59 miRNAs that were equally abundant between AIC and AKC. Integrative analysis of the mRNA and miRNA transcriptomes revealed macrophage heterogeneity in both AIC and AKC. In addition, analysis of AIC and AKC transcriptomes revealed functional characteristics of mucosal and systemic macrophages. Five pairs with significant negative correlations between miRNA and mRNAs were linked to macrophages and epithelial cells and their interaction could be pointing to macrophage activation and differentiation. The potential macrophage markers suggested in this study should be investigated under different immune conditions to understand the exact macrophage phenotypes. Still Image Atlantic salmon Unknown |
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topic |
Immunology Applied Immunology (incl. Antibody Engineering Xenotransplantation and T-cell Therapies) Autoimmunity Cellular Immunology Humoural Immunology and Immunochemistry Immunogenetics (incl. Genetic Immunology) Innate Immunity Transplantation Immunology Tumour Immunology Immunology not elsewhere classified Genetic Immunology Animal Immunology Veterinary Immunology adherent cells intestinal cells macrophages RNA-seq miRNAs Atlantic salmon |
spellingShingle |
Immunology Applied Immunology (incl. Antibody Engineering Xenotransplantation and T-cell Therapies) Autoimmunity Cellular Immunology Humoural Immunology and Immunochemistry Immunogenetics (incl. Genetic Immunology) Innate Immunity Transplantation Immunology Tumour Immunology Immunology not elsewhere classified Genetic Immunology Animal Immunology Veterinary Immunology adherent cells intestinal cells macrophages RNA-seq miRNAs Atlantic salmon Youngjin Park (195938) Qirui Zhang (555365) Jorge M. O. Fernandes (7381673) Geert F. Wiegertjes (6790895) Viswanath Kiron (252416) Image_2_Macrophage Heterogeneity in the Intestinal Cells of Salmon: Hints From Transcriptomic and Imaging Data.jpeg |
topic_facet |
Immunology Applied Immunology (incl. Antibody Engineering Xenotransplantation and T-cell Therapies) Autoimmunity Cellular Immunology Humoural Immunology and Immunochemistry Immunogenetics (incl. Genetic Immunology) Innate Immunity Transplantation Immunology Tumour Immunology Immunology not elsewhere classified Genetic Immunology Animal Immunology Veterinary Immunology adherent cells intestinal cells macrophages RNA-seq miRNAs Atlantic salmon |
description |
The intestine has many types of cells that are present mostly in the epithelium and lamina propria. The importance of the intestinal cells for the mammalian mucosal immune system is well-established. However, there is no in-depth information about many of the intestinal cells in teleosts. In our previous study, we reported that adherent intestinal cells (AIC) predominantly express macrophage-related genes. To gather further evidence that AIC include macrophage-like cells, we compared their phagocytic activity and morphology with those of adherent head kidney cells (AKC), previously characterized as macrophage-like cells. We also compared equally abundant as well as differentially expressed mRNAs and miRNAs between AIC and AKC. AIC had lower phagocytic activity and were larger and more circular than macrophage-like AKC. RNA-Seq data revealed that there were 18309 mRNAs, with 59 miRNAs that were equally abundant between AIC and AKC. Integrative analysis of the mRNA and miRNA transcriptomes revealed macrophage heterogeneity in both AIC and AKC. In addition, analysis of AIC and AKC transcriptomes revealed functional characteristics of mucosal and systemic macrophages. Five pairs with significant negative correlations between miRNA and mRNAs were linked to macrophages and epithelial cells and their interaction could be pointing to macrophage activation and differentiation. The potential macrophage markers suggested in this study should be investigated under different immune conditions to understand the exact macrophage phenotypes. |
format |
Still Image |
author |
Youngjin Park (195938) Qirui Zhang (555365) Jorge M. O. Fernandes (7381673) Geert F. Wiegertjes (6790895) Viswanath Kiron (252416) |
author_facet |
Youngjin Park (195938) Qirui Zhang (555365) Jorge M. O. Fernandes (7381673) Geert F. Wiegertjes (6790895) Viswanath Kiron (252416) |
author_sort |
Youngjin Park (195938) |
title |
Image_2_Macrophage Heterogeneity in the Intestinal Cells of Salmon: Hints From Transcriptomic and Imaging Data.jpeg |
title_short |
Image_2_Macrophage Heterogeneity in the Intestinal Cells of Salmon: Hints From Transcriptomic and Imaging Data.jpeg |
title_full |
Image_2_Macrophage Heterogeneity in the Intestinal Cells of Salmon: Hints From Transcriptomic and Imaging Data.jpeg |
title_fullStr |
Image_2_Macrophage Heterogeneity in the Intestinal Cells of Salmon: Hints From Transcriptomic and Imaging Data.jpeg |
title_full_unstemmed |
Image_2_Macrophage Heterogeneity in the Intestinal Cells of Salmon: Hints From Transcriptomic and Imaging Data.jpeg |
title_sort |
image_2_macrophage heterogeneity in the intestinal cells of salmon: hints from transcriptomic and imaging data.jpeg |
publishDate |
2021 |
url |
https://doi.org/10.3389/fimmu.2021.798156.s003 |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_relation |
https://figshare.com/articles/figure/Image_2_Macrophage_Heterogeneity_in_the_Intestinal_Cells_of_Salmon_Hints_From_Transcriptomic_and_Imaging_Data_jpeg/17426294 doi:10.3389/fimmu.2021.798156.s003 |
op_rights |
CC BY 4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.3389/fimmu.2021.798156.s003 |
_version_ |
1766363318911500288 |