Bis-ANS fluorescence of SpTrx WT and F26 mutants.
Temperature-induced unfolding of SpTrx WT and F26 mutants were measured using bis-ANS fluorescence after incubating the proteins (10 μM) at various temperatures (4–90°C) for 2 h (excitation at 385 nm). The data presented are the means of three experiments.
Main Authors: | , , , , , |
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Format: | Still Image |
Language: | unknown |
Published: |
2021
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Subjects: | |
Online Access: | https://doi.org/10.1371/journal.pone.0261123.g005 |
Summary: | Temperature-induced unfolding of SpTrx WT and F26 mutants were measured using bis-ANS fluorescence after incubating the proteins (10 μM) at various temperatures (4–90°C) for 2 h (excitation at 385 nm). The data presented are the means of three experiments. |
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